Enhanced Production of 2-Hydroxyphenazine from Glycerol by a Two-Stage Fermentation Strategy in <i>Pseudomonas chlororaphis</i> GP72AN

Yue, Sheng‐Jie; Huang, Peng; Li, Song; Jan, Malik; Hu, Hongbo; Wang, Wei; Zhang, Xuehong

doi:10.1021/acs.jafc.9b05033

Cited by 20 publications

(22 citation statements)

References 35 publications

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“…Overexpression of the phaG gene in Pseudomonas mendocina NK-U-2 slightly decreased the cell dry weight (CDW) but notably increased the mcl-PHA titer from 24.5 to 51.2 wt % . Nevertheless, most engineered strains show relatively low performance in both the CDW and the titer of mcl-PHA at the shake-flask level, which thereby hampers large-scale fermentation for the desired production. , In addition, engineering of Pseudomonas chlororaphis, which is a valuable chassis for the biosynthesis of diverse value-added products, − for improved mcl-PHA production is infrequently reported.…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis and Characterization of Medium-Chain-Length Polyhydroxyalkanoate with an Enriched 3-Hydroxydodecanoate Monomer from a Pseudomonas chlororaphis Cell Factory

Deng

Wang

et al. 2021

J. Agric. Food Chem.

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Polyhydroxyalkanoates (PHAs) have been reported with agricultural and medical applications in virtue of their biodegradable and biocompatible properties. Here, we systematically engineered three modules for the enhanced biosynthesis of medium-chain-length polyhydroxyalkanoate (mcl-PHA) in Pseudomonas chlororaphis HT66. The phzE, fadA, and fadB genes were deleted to block the native phenazine pathway and weaken the fatty acid β-oxidation pathway. Additionally, a PHA depolymerase gene phaZ was knocked out to prevent the degradation of mcl-PHA. Three genes involved in the mcl-PHA biosynthesis pathway were co-overexpressed to increase carbon flux. The engineered strain HT4Δ::C1C2J exhibited an 18.2 g/L cell dry weight with 84.9 wt % of mcl-PHA in a shake-flask culture, and the 3-hydroxydodecanoate (3HDD) monomer was increased to 71.6 mol %. Thermophysical and mechanical properties of mcl-PHA were improved with an enriched ratio of 3HDD. This study demonstrated a rational metabolic engineering approach to enhance the production of mcl-PHA with the enriched dominant monomer and improved material properties.

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Section: Introductionmentioning

confidence: 99%

Biosynthesis and Characterization of Medium-Chain-Length Polyhydroxyalkanoate with an Enriched 3-Hydroxydodecanoate Monomer from a Pseudomonas chlororaphis Cell Factory

Deng

Wang

et al. 2021

J. Agric. Food Chem.

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“…Among the Pseudomonas biocontrol strains, the ability to produce antibiotics is an effective method for controlling plant diseases. Phenazine-1-carboxylic acid (PCA) produced by Pseudomonas species provides the effective suppression of pathogens [ 13 ], such as Gaeumannomyces graminis which causes take-all disease of wheat [ 14 ]. PCA is the main active component of the benzimidazole-derived biopesticide “Shenqinmycin” [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

The Role of Pyoluteorin from Pseudomonas protegens Pf-5 in Suppressing the Growth and Pathogenicity of Pantoea ananatis on Maize

Qiao

Wang

et al. 2022

IJMS

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The rhizospheric bacterium Pseudomonas protegens Pf-5 can colonize the seed and root surfaces of plants, and can protect them from pathogen infection. Secondary metabolites, including lipopeptides and polyketides produced by Pf-5, are involved in its biocontrol activity. We isolated a crude extract from Pf-5. It exhibited significant surface activity and strong antibacterial activity against Pantoea ananatis DZ-12, which causes maize brown rot on leaves. HPLC analysis combined with activity tests showed that the polyketide pyoluteorin in the crude extract participated in the suppression of DZ-12 growth, and that the lipopeptide orfamide A was the major biosurfactant in the crude extract. Further studies indicated that the pyoluteorin in the crude extract significantly suppressed the biofilm formation of DZ-12, and it induced the accumulation of reactive oxygen species in DZ-12 cells. Scanning electron microscopy and transmission electron microscopy observation revealed that the crude extract severely damaged the pathogen cells and caused cytoplasmic extravasations and hollowing of the cells. The pathogenicity of DZ-12 on maize leaves was significantly reduced by the crude extract from Pf-5 in a dose-dependent manner. The polyketide pyoluteorin had strong antibacterial activity against DZ-12, and it has the potential for development as an antimicrobial agent.

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“…6 2-Hydroxyphenazine (2-OH-PHZ) is another effective biocontrol antibiotic, which has a better inhibitory effect on the take-all disease of wheat than PCA. 2,7 The take-all disease of wheat is one of the most serious diseases in the rhizosphere of wheat, and there is still a lack of effective control measures. 8,9 Therefore, 2-OH-PHZ is a promising candidate to develop a biopesticide to control the take-all disease of wheat.…”

Section: Introductionmentioning

confidence: 99%

“…Phenazines are a series of heterocyclic nitrogen-containing compounds, which are widely used in the field of biological control due to their high-efficiency broad-spectrum antifungal activity . Moreover, phenazines also exhibit the functions of anti-tumor, anti-cancer, and anti-parasitic effects, as an electron carrier, etc., attracting more and more researchers’ attention. − The most typical phenazine is phenazine-1-carboxylic acid (PCA) registered as a novel biosynthetic fungicide “Shenqinmycin” by the Ministry of Agriculture of China in 2011. , PCA at a concentration of 1% showed highly effective activities in preventing pepper blight, rice sheath blight, and cucumber seedling damping-off . 2-Hydroxyphenazine (2-OH-PHZ) is another effective biocontrol antibiotic, which has a better inhibitory effect on the take-all disease of wheat than PCA. , The take-all disease of wheat is one of the most serious diseases in the rhizosphere of wheat, and there is still a lack of effective control measures. , Therefore, 2-OH-PHZ is a promising candidate to develop a biopesticide to control the take-all disease of wheat.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Engineering of Pseudomonas chlororaphis LX24 with High Production of 2-Hydroxyphenazine

Liu

Yue

Feng

et al. 2021

J. Agric. Food Chem.

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The take-all disease of wheat is one of the most serious diseases in the field of food security in the world. There is no effective biological pesticide to prevent the take-all disease of wheat. 2-Hydroxyphenazine (2-OH-PHZ) was reported to possess a better inhibitory effect on the take-all disease of wheat than phenazine-1-carboxylic acid, which was registered as “Shenqinmycin” in China in 2011. The aim of this study was to construct a 2-OH-PHZ high-producing strain by strain screening, genome sequencing, genetic engineering, and fermentation optimization. First, the metabolites of the previously screened new phenazine-producing Pseudomonas sp. strain were identified, and the taxonomic status of the new Pseudomonas sp. strain was confirmed through 16S rRNA and matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS). Then, the new Pseudomonas sp. strain was named Pseudomonas chlororaphis subsp. aurantiaca LX24, which is a new subspecies of P. chlororaphis that can synthesize 2-OH-PHZ. Next, the draft genome of strain LX24 was determined, and clusters of orthologous group (COG) analysis, KEGG analysis, and gene ontology (GO) analysis of strain LX24 were performed. Furthermore, the production of 2-OH-PHZ increased to 351.7 from 158.6 mg/L by deletion of the phenazine synthesis negative regulatory genes rpeA and rsmE in strain LX24. Finally, the 2-OH-PHZ production of strain LX24 reached 677.1 mg/L after fermentation optimization, which is the highest production through microbial fermentation reported to date. This work provides a reference for the efficient production of other pesticides and antibiotics.

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Enhanced Production of 2-Hydroxyphenazine from Glycerol by a Two-Stage Fermentation Strategy in Pseudomonas chlororaphis GP72AN

Cited by 20 publications

References 35 publications

Biosynthesis and Characterization of Medium-Chain-Length Polyhydroxyalkanoate with an Enriched 3-Hydroxydodecanoate Monomer from a Pseudomonas chlororaphis Cell Factory

Biosynthesis and Characterization of Medium-Chain-Length Polyhydroxyalkanoate with an Enriched 3-Hydroxydodecanoate Monomer from a Pseudomonas chlororaphis Cell Factory

The Role of Pyoluteorin from Pseudomonas protegens Pf-5 in Suppressing the Growth and Pathogenicity of Pantoea ananatis on Maize

Characterization and Engineering of Pseudomonas chlororaphis LX24 with High Production of 2-Hydroxyphenazine

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