Enhanced production of biosynthesized lycopene via heterogenous MVA pathway based on chromosomal multiple position integration strategy plus plasmid systems in Escherichia coli

Wei, Yanlong; Mohsin, Ali; Hong, Quan; Guo, Meijin; Fang, Hongqing

doi:10.1016/j.biortech.2017.11.035

Cited by 29 publications

(26 citation statements)

References 37 publications

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“…Cells were grown at 30 °C and helper plasmid curing was done at 42 °C. For lycopene production, cells were cultured at 220 rpm at 30 °C in test tube with 5 mL FMGAT medium (15 g/L tryptone, 12 g/L yeast extract, 5 g/L NaH 2 PO 4 ·2H 2 O, 7 g/L K 2 HPO 4 ·3H 2 O, 2.5 g/L NaCl, 5 g/L Tween 80, 10 g/L glycerol, 0.5 g/L MgSO 4 , 2 g/L glucose, and 2 g/L l -arabinose), and original inoculum concentration was 0.05 (OD 600nm ) [ 49 ]. Ampicillin (50 μg/ml), chloramphenicol (25 μg/ml), and kanamycin (25 μg/ml) were added for plasmid maintenance.…”

Section: Methodsmentioning

confidence: 99%

“…Thus, the T7 RNA polymerase was under the control of araC -P BAD , and l -arabinose could be used as inductor to produce lycopene. However, its specific lycopene production was 0.66 mg/g DCW (dry cell weight), which was considered very low [ 49 ]. In order to improve the lower productivity, many previous studies have shown that chromosomal positioning has a notable impact on gene transcription to get the desired level of product [ 50 , 51 ].…”

Section: Introductionmentioning

confidence: 99%

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Rationally optimized generation of integrated Escherichia coli with stable and high yield lycopene biosynthesis from heterologous mevalonate (MVA) and lycopene expression pathways

Hussain

Hong

Zaman

et al. 2021

Synthetic and Systems Biotechnology

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rationally optimized generation of integrated Escherichia coli with stable and high yield lycopene biosynthesis from heterologous mevalonate (MVA) and lycopene expression pathways

Hussain

Hong

Zaman

et al. 2021

Synthetic and Systems Biotechnology

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“…Recently, based on an engineered lipid overproducing strain of Y. lipolytica, Larroude et al have rewired it to produce at 6.5 g/L and 90 mg/g DCW of β-carotene [47]. These results are relatively better than those previously achieved [48][49][50]. It would not be surprising if some of these examples would lead to the successful commercialization notably of novel β-carotene sources in the near future.…”

Section: Biosynthesis Of Carotenesmentioning

confidence: 99%

Biosynthesis of Carotenoids and Apocarotenoids by Microorganisms and Their Industrial Potential

Zhang¹

2018

Progress in Carotenoid Research

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Carotenoids are a large group of natural pigments, ranging from red, to orange, to yellow colors. Synthesized by plants and some microorganisms (e.g., microalgae, fungi and bacteria), carotenoids have important physiological functions (e.g., light harvesting). Apocarotenoids are carotenoid-derived compounds and play important roles in various biological activities (e.g., plant hormones). Many carotenoids and apocarotenoids have high economic value in feed, food, supplements, cosmetics and pharmaceutical industries. Despite high commercial values, they are severely undersupplied because of low abundance in natural hosts (usually a few milligrams per kilogram of raw materials). Furthermore, plants or microbes usually produce mixtures of these molecules with very similar physical and chemical properties (such as α-and β-carotenes). All these features render the extraction from natural hosts rather difficult and also very costly both from process economics and sustainable land-use viewpoints. Chemical synthesis is also expensive due to structural complexity (e.g., astaxanthin has many unsaturated bonds and two chiral regions). Biotechnology via the rapidly advancing metabolic engineering and synthetic biology approaches has led to alternative ways to attain several carotenoids and apocarotenoids at relatively high titers and yields using fast-growing microorganisms. This chapter briefly reviews the biosynthesis of carotenoids and apocarotenoids by microorganisms and their industrial potential.

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“…Lycopene is a C40 isoprenoid compound in the carotenoid family. Due to its anti-oxidative and anti-cancer activities ( Sies and Stahl, 1998 ; Gajowik and Dobrzynska, 2014 ), lycopene has been widely used for nutritional supplements, pharmaceutical and cosmetic products ( Wei et al, 2017 ). The conventional methods for lycopene production include direct extraction from plants,chemical synthesis and microbial fermentation.…”

Section: Introductionmentioning

confidence: 99%

“…Among these methods, microbial production of lycopene is more economical and sustainable ( Chen et al, 2016 ). Recently, with the development of metabolic engineering techniques and synthetic biology, lycopene overproduction has been realized in Escherichia coli ( Zhu et al, 2015 ; Wei et al, 2017 ; Wu et al, 2018 ; Xu et al, 2018 ), yeast ( Xie et al, 2015a ; Schwartz et al, 2017 ), Blakeslea trispora ( Liu et al, 2012 ; Wang et al, 2016 , 2017 ), and Rhodobacter sphaeroides ( Su et al, 2018 ). However, the field is seeking a better platform for large-scale production of lycopene or other carotenoids.…”

Section: Introductionmentioning

confidence: 99%

Engineering Haloferax mediterranei as an Efficient Platform for High Level Production of Lycopene

et al. 2018

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Lycopene attracts increasing interests in the pharmaceutical, food, and cosmetic industries due to its anti-oxidative and anti-cancer properties. Compared with other lycopene production methods, such as chemical synthesis or direct extraction from plants, the biosynthesis approach using microbes is more economical and sustainable. In this work, we engineered Haloferax mediterranei, a halophilic archaeon, as a new lycopene producer. H. mediterranei has the de novo synthetic pathway for lycopene but cannot accumulate this compound. To address this issue, we reinforced the lycopene synthesis pathway, blocked its flux to other carotenoids and disrupted its competitive pathways. The reaction from geranylgeranyl-PP to phytoene catalyzed by phytoene synthase (CrtB) was identified as the rate-limiting step in H. mediterranei. Insertion of a strong promoter PphaR immediately upstream of the crtB gene, or overexpression of the heterologous CrtB and phytoene desaturase (CrtI) led to a higher yield of lycopene. In addition, blocking bacterioruberin biosynthesis increased the purity and yield of lycopene. Knock-out of the key genes, responsible for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) biosynthesis, diverted more carbon flux into lycopene synthesis, and thus further enhanced lycopene production. The metabolic engineered H. mediterranei strain produced lycopene at 119.25 ± 0.55 mg per gram of dry cell weight in shake flask fermentation. The obtained yield was superior compared to the lycopene production observed in most of the engineered Escherichia coli or yeast even when they were cultivated in pilot scale bioreactors. Collectively, this work offers insights into the mechanism involved in carotenoid biosynthesis in haloarchaea and demonstrates the potential of using haloarchaea for the production of lycopene or other carotenoids.

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Enhanced production of biosynthesized lycopene via heterogenous MVA pathway based on chromosomal multiple position integration strategy plus plasmid systems in Escherichia coli

Cited by 29 publications

References 37 publications

Rationally optimized generation of integrated Escherichia coli with stable and high yield lycopene biosynthesis from heterologous mevalonate (MVA) and lycopene expression pathways

Rationally optimized generation of integrated Escherichia coli with stable and high yield lycopene biosynthesis from heterologous mevalonate (MVA) and lycopene expression pathways

Biosynthesis of Carotenoids and Apocarotenoids by Microorganisms and Their Industrial Potential

Engineering Haloferax mediterranei as an Efficient Platform for High Level Production of Lycopene

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