Enhanced production of cell-bound cholesterol oxidase from Rhodococcus sp. NCIM 2891 by the statistical method

Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94

“…El-Naggar et al [14] reported that the optimal levels of the three selected process factors for maximum cholesterol oxidase production (20.521 U/mL) as obtained from the central composite design were pH 8; cholesterol concentration 3 g/L; (NH 4 ) 2 SO 4 8 g/L. However, Ahmad and Goswami [42] optimized the medium for production of cholesterol oxidase by Rhodococcus sp. NCIM 2891 using the classical and statistical methods.…”

Cholesterol Oxidase: Source,Properties and Applications.

“…Ammonium chloride and ammonium acetate have been reported to show highest effect on CHOx production by R. equi no 23 [47] and A. simplex [34], respectively. Another study also reported that the combination of (NH 4 ) 2 HPO 4 and yeast extract provided the highest enzyme production as compared to individual organic and inorganic components [52].…”

Application of chitosan beads immobilized Rhodococcus sp. NCIM 2891 cholesterol oxidase for cholestenone production

“…NCIM 2891 and the enzyme production were based on a previous work [12]. Briefly, the medium contained the following (in g/L): cholesterol, 3.5; (NH 4 ) 2 HPO 4 , 9.0; yeast extract, 22.0; K 2 HPO 4 , 0.25; FeSO 4 ·7H 2 O, 0.1; NaCl, 1.0; Tween 80, 2.0 (v/v), pH 7.0.…”

“…Cholestenone is generally produced through biotransformation route using microbial cells as catalysts [9,10] with limited report where enzyme has been also used as catalyst [11]. A variety of microorganisms produce cholesterol oxidase, among which Rhodococcus species have attracted wide attention for their high production capability of the enzyme [3,4,12]. Both secreted and cell-bound forms of the enzyme are produced by many Rhodococcus strains [12][13][14][15].…”

“…A variety of microorganisms produce cholesterol oxidase, among which Rhodococcus species have attracted wide attention for their high production capability of the enzyme [3,4,12]. Both secreted and cell-bound forms of the enzyme are produced by many Rhodococcus strains [12][13][14][15]. The molecular characteristics of the cholesterol oxidase from Rhodococcus strains have been explored to some extent [16][17][18][19][20][21], while the physico-chemical properties of the enzyme which are relevant to the bioprocess development are yet to be adequately elucidated.…”