Enhanced production of flavonoids by methyl jasmonate elicitation in cell suspension culture of Hypericum perforatum

Wang, Jing; Qian, Jie; Yao, Lingyun; Lü, Yanhua

doi:10.1186/s40643-014-0033-5

Cited by 130 publications

(76 citation statements)

References 38 publications

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“…A decline in CAT activity under CHI elicitation suggested a possible delay in removal of toxic peroxides, which could lead to a higher ROS accumulation resulting in a stimulation of phenylpropanoid and naphtodianthrone production. Such an observation is in agreement with the theory of ROS-mediated accumulation of naphtodianthrones (Danova et al 2012) and flavonoids (Wang et al 2015) in Hypericum in vitro cultures. When antioxidant enzymes in PEC elicited cells were compared, it was observed that higher CAT activity was more prolonged than that of POD.…”

Section: Antioxidant Activity Of H Perforatum Cell Suspensionssupporting

confidence: 87%

Polysaccharide elicitors enhance phenylpropanoid and naphtodianthrone production in cell suspension cultures of Hypericum perforatum

Simic

Tusevski

Maury

et al. 2015

Plant Cell Tiss Organ Cult

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Hypericum perforatum cell suspensions were evaluated for their growth, phenylpropanoid and naphtodianthrone productions, and antioxidant activity after treatments with polysaccharide elicitors chitin (CHI), pectin (PEC) and dextran (DEX). Polysaccharide elicitation of cell cultures showed a considerable improving effect on phenylpropanoid production (phenolics, flavonoids, flavanols and anthocyanins) during the post-elicitation period. Even that enhanced phenylpropanoid production was related to growth suppression of CHI elicited cells, PEC and DEX were presented as effective elicitors without loss of cell biomass. Phenylpropanoid accumulation in elicited cells was correlated to markedly higher enzyme activities of phenylalanine ammonia lyase and chalcone-flavanone isomerase. Cell cultures were found to respond rapidly towards the applied elicitors through an early stimulation of both naphtodiantrones, hypericin (HYP) and pseudohypericin (PHYP). The CHI was proposed as a much more prominent elicitor in stimulation of HYP and PHYP levels in cell suspensions. All tested polysaccharide elicitors stimulated non-enzymatic antioxidant properties in cell extracts indicating the potential role of phenolic compounds as effective antioxidants. With regards to the antioxidant enzymes, an up-regulation of peroxidase and catalase activities was observed in PEC and DEX elicited cells, whereas CHI did not give any stimulatory effect on enzymatic activities. These findings suggest the involvement of an efficient antioxidant defense system in the adaptive response of cells to polysaccharide elicitation. Altogether, these results indicated that H. perforatum cells elicited with polysaccharides represent a promising experimental system for enhanced production of phenylpropanoids and naphtodianthrones.

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Section: Antioxidant Activity Of H Perforatum Cell Suspensionssupporting

confidence: 87%

Polysaccharide elicitors enhance phenylpropanoid and naphtodianthrone production in cell suspension cultures of Hypericum perforatum

Simic

Tusevski

Maury

et al. 2015

Plant Cell Tiss Organ Cult

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“…The increased synthesis of flavonoids due to JA supplementation supports the findings by Horbowicz et al (2011), Ishihara et al (2002, and Wang et al (2015). Xu et al (2006) have reported that NO production induces the synthesis of JA, SA, and secondary metabolites, thereby mediating the regulated crosstalk between JA, SA, secondary metabolites, and NO signaling, for better stress amelioration.…”

Section: Discussionsupporting

confidence: 71%

Mitigation of sodium chloride toxicity inSolanum lycopersicumL. by supplementation of jasmonic acid and nitric oxide

Ahmad

Ahanger

Alyemeni

et al. 2018

Journal of Plant Interactions

158

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We investigated the effects of exogenous application of jasmonic acid (JA) and nitric oxide (NO) on growth, antioxidant metabolism, physio-biochemical attributes and metabolite accumulation, in tomato (Solanum lycopersicum L.) plants exposed to salt stress. Treating the plants with NaCl (200 mM) resulted in considerable growth inhibition in terms of biomass, relative water content, and chlorophyll content, all of which were significantly improved upon application of JA and NO under both normal and NaCl-stress treatments. Salt treatment particularly 200 mM NaCl caused an apparent increase in electrolyte leakage, lipid peroxidation, and hydrogen peroxide production, which were reduced by exogenous application of JA and NO. Salt treatment triggered the induction of antioxidant system by enhancing the activities of antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR). Application of JA and NO separately as well as in combination caused a significant improvement in activities of SOD, CAT, APX, and GR activities. JA and NO either applied individually or in combination boosted the flavonoid, proline and glycine betaine synthesis under NaCl treatments. In conclusion, the exogenous application of JA and NO protected tomato plants from NaCl-induced damage by up-regulating the antioxidant metabolism, osmolyte synthesis, and metabolite accumulation. ARTICLE HISTORY

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“…The contents of rutin, quercetin myricetin, hesperidin, formononetin, biochanin A, kaempferol, and naringenin were higher in the JA, SA, and PGR cell cultures than in the control cell cultures. Flavonoid content was stimulated by MeJA and SA induction, which were 2.1 and 1.5 times higher when compared with the control cell suspension cultures of Hypericum perforatum [35]. The levels of pyrogallol, resveratrol, veratric acid, and vanillin were higher in the JA, SA, and PGR cell cultures than in the control cell cultures (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Enhanced Production of Anthraquinones and Phenolic Compounds and Biological Activities in the Cell Suspension Cultures of Polygonum multiflorum

Thiruvengadam

Rekha

Rajakumar

et al. 2016

IJMS

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Anthraquinones (AQs) and phenolic compounds are important phytochemicals that are biosynthesized in cell suspension cultures of Polygonum multiflorum. We wanted to optimize the effects of plant growth regulators (PGRs), media, sucrose, l-glutamine, jasmonic acid (JA), and salicylic acid (SA) for the production of phytochemicals and biomass accumulation in a cell suspension culture of P. multiflorum. The medium containing Murashige and Skoog (MS) salts and 4% sucrose supplemented with 1 mg/L 2,4-dichlorophenoxyacetic acid, 0.5 mg/L thidiazuron, and 100 µM l-glutamine at 28 days of cell suspension culture was suitable for biomass accumulation and AQ production. Maximum biomass accumulation (12.5 and 12.35 g fresh mass (FM); 3 and 2.93 g dry mass (DM)) and AQ production (emodin 295.20 and 282 mg/g DM; physcion 421.55 and 410.25 mg/g DM) were observed using 100 µM JA and SA, respectively. JA- and SA-elicited cell cultures showed several-fold higher biomass accumulation and AQ production than the control cell cultures. Furthermore, the cell suspension cultures effectively produced 23 phenolic compounds, such as flavonols and hydroxycinnamic and hydroxybenzoic acid derivatives. PGR-, JA-, and SA-elicited cell cultures produced a higher amount of AQs and phenolic compounds. Because of these metabolic changes, the antioxidant, antimicrobial, and anticancer activities were high in the PGR-, JA-, and SA-elicited cell cultures. The results showed that the elicitors (JA and SA) induced the enhancement of biomass accumulation and phytochemical (AQs and phenolic compounds) production as well as biological activities in the cell suspension cultures of P. multiflorum. This optimized protocol can be developed for large-scale biomass accumulation and production of phytochemicals (AQs and phenolic compounds) from cell suspension cultures, and the phytochemicals can be used for various biological activities.

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Enhanced production of flavonoids by methyl jasmonate elicitation in cell suspension culture of Hypericum perforatum

Cited by 130 publications

References 38 publications

Polysaccharide elicitors enhance phenylpropanoid and naphtodianthrone production in cell suspension cultures of Hypericum perforatum

Polysaccharide elicitors enhance phenylpropanoid and naphtodianthrone production in cell suspension cultures of Hypericum perforatum

Mitigation of sodium chloride toxicity inSolanum lycopersicumL. by supplementation of jasmonic acid and nitric oxide

Enhanced Production of Anthraquinones and Phenolic Compounds and Biological Activities in the Cell Suspension Cultures of Polygonum multiflorum

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