Enhanced production of fructose ester by biocatalyzed continuous flow process

Abstract

Background: Fatty acid sugar esters are non-toxic, odorless, non-irritanting surfactants. They can be synthesized by renewable resources and are completely biodegradable in aerobic and anaerobic conditions. Their application has been expanded in innumerous areas including pharmaceuticals, cosmetics, detergents and food industry. Lipase-catalyzed esterification have been investigated as a potential substitute to the traditional chemical, demanding milder reaction conditions, allowing better reaction control and providing higher-quality products. So, the lipase catalyzed sugar ester synthesis becomes an interesting strategy for producing biodegradable, non-ionic surfactants. The main disadvantage of this protocol is the poor solubility of substrates and long reaction time required for performed the esterification reaction with moderated to good yields.Results: Here in, we report the enzymatic esterification of steric hindered fructose derivative with free fatty acids derived from palm oil refining process (RePO) under continuous flow conditions at concentrations up to 0.5 M, increasing the productivity up to 100 mg. min −1 .g immob. enzyme −1 . Conclusions:The immobilized commercial enzyme from Rhizomucor miehei generated the best performance between the catalysts tested.

Continuous Flow Alcoholysis of Dialkyl H-Phosphonates with Aliphatic Alcohols

“…The most common preparations of simple carboxylic esters comprise the acid-catalyzed reaction of a carboxylic acid with alcohol (Fischer esterification) and the reaction of an ester with alcohol (alcoholysis) [ 10 ]. Continuous flow Fischer esterifications may be carried out in systems containing a packed catalyst bed [ 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ] or a heated coil [ 21 , 22 ], as well as in microreactors [ 23 ]. In a few cases, the esterifications were performed in continuous flow MW systems based on professional MW reactors [ 24 , 25 , 26 ] or in household MW ovens [ 27 , 28 ].…”

Biocatalysis in continuous-flow mode: A case-study in the enzymatic kinetic resolution of secondary alcohols via acylation and deacylation reactions mediated by Novozym 435®

“…Aldolases [10], amidases [11], transaminases [12], oxidases [13,14], peroxidases [15] are some enzymes that have been successfully applied to a continuous-flow approach. Additionally, lipase-mediated esterification [16][17][18][19][20][21], interesterification [22,23], transesterification [24][25][26] and enzymatic kinetic resolution (EKR) of racemic compounds [27][28][29][30][31][32], have demonstrated the potential of combining continuous-flow systems and biocatalysis.…”