Enhanced production of heterologous proteins by the filamentous fungus Trichoderma reesei via disruption of the alkaline serine protease SPW combined with a pH control strategy

Zhang, Guoxiu; Zhu, Yao lei; Wei, Dongzhi; Wang, Wei

doi:10.1016/j.plasmid.2014.01.001

Cited by 32 publications

(29 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The activity of acidic proteases was shown to decrease steadily at a pH greater than 3.0 and was significantly lower between pH 5.0 and 6.0 for A. niger culture broth [114]. The activity of alkaline proteases could be reduced by gene disruption [115].…”

Section: Proteolytic Degradation Of Heterologously Expressed Cellobiomentioning

confidence: 98%

Heterologous expression of cellobiohydrolases in filamentous fungi – An update on the current challenges, achievements and perspectives

Zoglowek

Lübeck

Ahring

et al. 2015

Process Biochemistry

View full text Add to dashboard Cite

Section: Proteolytic Degradation Of Heterologously Expressed Cellobiomentioning

confidence: 98%

Heterologous expression of cellobiohydrolases in filamentous fungi – An update on the current challenges, achievements and perspectives

Zoglowek

Lübeck

Ahring

et al. 2015

Process Biochemistry

View full text Add to dashboard Cite

“…In submerged cultivations product degradation by proteases can result from autolysis and fragmentation of hyphae, which frequently occur toward the end of the stationary phase [112,113]. Heterologous protein production in industry often utilize protease deficient strains, and recently a study showed that heterologous EG production could be increased by deletion of a major alkaline protease gene [114]. Micro-colonies formed under submerged conditions have been shown to be heterogeneous with regards to morphology and gene expression level of A. niger glucoamylase [115].…”

Section: Advantages and Challenges With Smfmentioning

confidence: 99%

Production of cellulolytic enzymes from ascomycetes: Comparison of solid state and submerged fermentation

Hansen

Lübeck

Frisvad

et al. 2015

Process Biochemistry

153

View full text Add to dashboard Cite

“…[29] T. reesei produces a broad series of cellulases, including exoglucanase (e.g. CBH I and CBH II), endoglucanases (e.g.…”

Section: Cellulase Production By T Reeseimentioning

confidence: 99%

Trichoderma reesei, a superior cellulase source for industrial applications

Keshavarz

Khalesi

2016

Biofuels

View full text Add to dashboard Cite

Trichoderma reesei, a superior cellulase source for industrial applications, is a genus of filamentous fungi with an exceptional potential to produce various enzymes and proteins including cellulases, hemicellulases, and hydrophobins. Growing on easy-access low-cost substrates, as well as a broad range of products produced by T. reesei, represent this genus as the first option for bio-industries to produce required enzymes and proteins with an efficient direction of bioprocess. Recently, many researchers have made efforts to improve the yield of T. reesei cellulases using recombinant strains, optimization of the substrates, and applying the synergistic effects of co-cultivation using different microorganisms. The current findings may help industries such as biofuel producers to reduce the cost of cellulase production by up to 40%. In this review, recent advances in last five years regarding the improvement in cellulase productivity using T. reesei have been pointed out and the highlights have been summarized.

show abstract

Enhanced production of heterologous proteins by the filamentous fungus Trichoderma reesei via disruption of the alkaline serine protease SPW combined with a pH control strategy

Cited by 32 publications

References 33 publications

Heterologous expression of cellobiohydrolases in filamentous fungi – An update on the current challenges, achievements and perspectives

Heterologous expression of cellobiohydrolases in filamentous fungi – An update on the current challenges, achievements and perspectives

Production of cellulolytic enzymes from ascomycetes: Comparison of solid state and submerged fermentation

Trichoderma reesei, a superior cellulase source for industrial applications

Contact Info

Product

Resources

About