Enhanced production of isoamyl acetate from beet molasses with addition of fusel oil by Williopsis saturnus var. saturnus

Yılmaztekin, Murat; Erten, Hüseyin; Cabaroğlu, Turgut

doi:10.1016/j.foodchem.2008.05.079

Cited by 47 publications

(50 citation statements)

References 11 publications

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“…9 Owing to its rich alcohol composition and availability in large quantities, fusel oil can be considered an inexpensive source of starting material for the production of important natural favor compounds. 8 The enzymatic synthesis of isoamyl butyrate was carried out using Lipozyme TL IM in an organic media.…”

Section: Model Fitting 2 3 Central Composite Rotatable Design (Ccrd)mentioning

confidence: 99%

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Enzymatic synthesis optimization of isoamyl butyrate

Anschau¹,

Aragão²,

Porciuncula³

et al. 2011

J. Braz. Chem. Soc.

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Lipozyme TL IM foi usada para catalisar a esterificação de álcool isoamílico e ácido butírico. Um planejamento fatorial fracionário foi utilizado para avaliar os efeitos da temperatura (30, 40, 50 ºC), razão molar álcool:ácido (1:1, 2:1, 3:1), concentração de enzima (0,003, 0,0115, 0,020 g mL -1 ), concentração de ácido butírico (0,1, 0,3, 0,5 mol L -1 ) e agitação (50, 115, 180 rpm) na produção de éster. Com esses resultados, os níveis foram redefinidos para um planejamento fatorial 2³. A máxima produção de éster foi obtida a 30 ºC, 180 rpm, razão molar álcool:ácido de 1:1, concentração de enzima 0,021 g mL -1 e concentração de ácido butírico de 0,5 mol L -1 . Sob essas condições ótimas foi obtido 92% de esterificação e concentração de éster de 0,9 mol L -1 . Álcool isoamílico obtido a partir de óleo fúsel foi utilizado nas mesmas condições resultando em 93% de esterificação e concentração de éster de 1,0 mol L -1 .Lipozyme TL IM was used to catalyse the esterification of isoamyl alcohol and butyric acid. A fractional factorial design was employed to evaluate the effects of temperature (30, 40, 50 °C), alcohol:acid molar ratio (1:1, 2:1, 3:1), enzyme concentration (0.003, 0.0115, 0.020 g mL -1 ), butyric acid concentration (0.1, 0.3, 0.5 mol L -1 ) and shaking rate (50, 115, 180 rpm) on the ester yield. With these results, the levels were redefined to a 2 3 factorial design. The maximum yield of ester was obtained at 30 ºC, 180 rpm, alcohol:acid molar ratio of 1:1, enzyme concentration of 0.021 g mL -1 and butyric acid concentration of 0.5 mol L -1 . Under the optimal conditions, 92% esterification was attained with an ester concentration of 0.9 mol L -1 . Isoamyl alcohol from fusel oil was used under the same conditions and resulted in 93% esterification and an ester concentration of 1.0 mol L -1 .

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Section: Model Fitting 2 3 Central Composite Rotatable Design (Ccrd)mentioning

confidence: 99%

“…7 However, a great part of the fusel oil produced is generally discarded. 9 Its average composition is 51% i-amyl-alcohol, 15% i-butanol, 13% n-propanol, 10% ethanol, 11% miscellaneous alcohols and water. 10 Here, we wish to report a study employing isoamyl alcohol from fusel oil instead of pure isoamyl alcohol.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic synthesis optimization of isoamyl butyrate

Anschau¹,

Aragão²,

Porciuncula³

et al. 2011

J. Braz. Chem. Soc.

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“…Isoamyl alcohol was added to the medium at 48. h of biotransformation and continued with 24 h intervals up to the end of biotransformation. The strategy for fed-batch biotransformation was to avoid the inhibition of isoamyl alcohol on the yeast, which was observed in our previous work [7]. Addition of small amounts of isoamyl alcohol at intervals of 24 h led to a 2.2 fold increase in isoamyl acetate concentration in fed-batch biotransformation (64 mg/L) compared to batch transformation (28.5 mg/L).…”

Section: Fed-batch Biotransformation With Adsorption Resinsmentioning

confidence: 83%

“…This indicated that large amounts of isoamyl alcohol in the medium negatively affected yeast growth. Since the biotransformation of isoamyl alcohol to isoamyl acetate is strictly growth-associated, yeast growth could act as an indicator of isoamyl acetate production during the process [7]. In the experiments carried out with addition of resins, the total isoamyl acetate concentration (isoamyl acetate in aqueous phase plus adsorbed onto the resin) exceeded that without addition of resin.…”

Section: Fed-batch Biotransformation With Adsorption Resinsmentioning

confidence: 99%

“…Acetate esters, such as ethyl acetate, hexyl acetate, isoamyl acetate and 2-phenylethyl acetate are recognised as important flavour compounds [3]. Especially, isoamyl acetate, the characteristic compound of banana flavour, is produced with an amount of 74 tonnes per annum [7]. Several yeasts are capable of producing large amounts of fruity ester flavours.…”

Section: Introductionmentioning

confidence: 99%

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Lindnera saturnus Kullanılarak Hücresel Biyodönüşüm Yolu ile Fuzel Yağından Muz Aroması Eldesi

Yılmaztekin¹,

Tay²

2016

Çukurova Üniversitesi Mühendislik-Mimarlık Fakültesi Dergisi

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An efficient biotransformation of isoamyl alcohol obtained from fusel oil to isoamyl acetate in a molasses based medium via in situ product removal (ISPR) with macroporous adsorption resin was carried out with Lindnera saturnus. Nine types of macroporous adsorption resins with different polarities and surface areas were tested with synthetic medium and then in batch and fed-batch cultivations. H103 resin had the best adsorption capacity because of its large and nonpolar surface areas. The isoamyl acetate concentration was increased with 42 and 30 folds in batch and fed-batch cultivations, respectively, compared to the biotransformations without addition of adsorbent resin. When 1 g H103 resin (wet w/v) was added to 50 mL of the biotransformation medium, the total isoamyl acetate concentration achieved was 1.9 g/L, of which 123 mg/L remained in the aqueous phase and 1787 mg/L was adsorbed onto the resin, within 120 h in fed-batch system. This was the highest isoamyl acetate yield by biotransformation until now and was remarkable for making the process more feasible for industrial application.

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