Enhanced production of l(+)-lactic acid from corn starch in a culture of Rhizopus oryzae using an air-lift bioreactor

ORIGINAL RESEARCH: Direct fermentation oftriticale starch to lactic acid byRhizopus oryzae

“…25 Up to 85 g LA was produced from 100 g corn starch in 3 L airlift bioreactors when the starch was prehydrolyzed by α-amylase, also using freshly prepared mycelium from spores as inoculum. 26 In the DASGIP bioreactor system described herein, our study shows that simultaneous saccharification and fermentation of triticale starch by R. oryzae spores produced 86.7±4.3 g LA from 100 g starting starch material -a yield that was about 10% higher than those obtained in shake flasks.…”

“…25 Several other studies involved the use of CaCO 3 , as well as experimenting on the time of addition, dosage, and ratio of fermentation substrate-to-CaCO 3 . 11,23,[26][27][28][29] Our results indicated that time of addition together with dosage of CaCO 3 was an important factor for LA production. Addition of CaCO 3 immediately at the beginning of fermentation was not a good strategy, as it actually reduced LA yields compared to the control conditions.…”

Organic Acid Production by Filamentous Fungi

“…Process parameter optimization leading to consistent production of small pellets would probably be the most economical means of obtaining a high-yielding strain. A number of studies have reported yields (w/w) of 85% to 88% Longacre et al, 1997;Yin et al, 1997;Du et al, 1998;Zhou et al, 1999). These yields are comparable to the yields routinely obtained with the bacterial process, and if consistently obtained, would contribute greatly to the economic competitiveness of the fungal process.…”

Lactic Acid Production to Purification: A Review

“…The main sources of nitrogen are yeast extract and ammonium sulfate (Yin et al 1997;Zhou et al 1999;Jin et al 2003). In terms of industrial processes, the use of yeast extract has a high cost, although it is best for the cultivation of lactic acid bacteria.…”