Enhanced production of lactic acid through the use of a novel aqueous two-phase system as an extractive fermentation system

Planas, Jordi; Rådström, Peter; Tjerneld, Folke; Hahn‐Hägerdal, Bärbel

doi:10.1007/s002530050756

Cited by 36 publications

(9 citation statements)

References 16 publications

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“…Dissing and Mattiesson, in 1994 [ 94 ], described ATPS containing a poly(ethyleneimine) (PEI) creating a lower phase accumulating lactate and hydroxyethylcellulose (HEC) at the top of the system extracting cells. Another two-phase system composed of ethylene oxide-propylene oxide/hydroxypropyl starch polymer-100 has been proposed by Planas et al [ 95 ] with a purpose to remove lactate produced by L. lactis subsp. lactis 19435.…”

Section: Toxicity Against Microorganismsmentioning

confidence: 99%

Ionic Liquids Toxicity—Benefits and Threats

Flieger

2020

IJMS

247

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Ionic liquids (ILs) are solvents with salt structures. Typically, they contain organic cations (ammonium, imidazolium, pyridinium, piperidinium or pyrrolidinium), and halogen, fluorinated or organic anions. While ILs are considered to be environmentally-friendly compounds, only a few reasons support this claim. This is because of high thermal stability, and negligible pressure at room temperature which makes them non-volatile, therefore preventing the release of ILs into the atmosphere. The expansion of the range of applications of ILs in many chemical industry fields has led to a growing threat of contamination of the aquatic and terrestrial environments by these compounds. As the possibility of the release of ILs into the environment s grow systematically, there is an increasing and urgent obligation to determine their toxic and antimicrobial influence on the environment. Many bioassays were carried out to evaluate the (eco)toxicity and biodegradability of ILs. Most of them have questioned their “green” features as ILs turned out to be toxic towards organisms from varied trophic levels. Therefore, there is a need for a new biodegradable, less toxic “greener” ILs. This review presents the potential risks to the environment linked to the application of ILs. These are the following: cytotoxicity evaluated by the use of human cells, toxicity manifesting in aqueous and terrestrial environments. The studies proving the relation between structures versus toxicity for ILs with special emphasis on directions suitable for designing safer ILs synthesized from renewable sources are also presented. The representants of a new generation of easily biodegradable ILs derivatives of amino acids, sugars, choline, and bicyclic monoterpene moiety are collected. Some benefits of using ILs in medicine, agriculture, and the bio-processing industry are also presented.

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Section: Toxicity Against Microorganismsmentioning

confidence: 99%

Ionic Liquids Toxicity—Benefits and Threats

Flieger

2020

IJMS

247

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“…The yield (1.1 mol/mol) and productivity (4.7 mM ⅐ h −1 , including phase separation time) obtained in the present investigation reflect the fact that pH was not controlled, but allowed to drop to 4.5. Similarly, in a regular batch fermentation without pH control, the yield was 0.74 mol/mol, and the productivity 2.5 mM ⅐ h −1 (Planas et al, 1996). The higher productivity obtained in the present study was because fermentation was carried out in ATPS allowing part of the lactic acid to be intermittently removed with the top phase.…”

Section: Discussionmentioning

confidence: 61%

“…Thus, the cell-containing phase is being reused, whereas the other phase is repeatedly renewed. This principle has been applied in so-called repeated extractive batch fermentations (Andersson and Hahn-Hägerdal, 1990;Chen and Lee, 1995;Planas et al, 1996). In polymer-polymer ATPS, each phase is composed of a mixture containing mainly one of the two phase forming polymers, and a lesser amount of the other polymer.…”

Section: Introductionmentioning

confidence: 99%

Analysis of phase composition in aqueous two-phase systems using a two-column chromatographic method: Application to lactic acid production by extractive fermentation

Planas¹,

Lefebvre²,

Tjerneld³

et al. 1997

Biotechnol. Bioeng.

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“…The relation between hydration of poly(ethylene oxide) in water, and temperature and salt concentration has long been known (Bailey and Koleske, 1976), but phase separation with changes in temperature and salt concentration have only recently been used for the recovery of proteins in aqueous two-phase systems (Alred et al, 1992(Alred et al, , 1994Harris et al, 1991;Johansson et al, 1996). The novel EOPO-PEI conjugate displayed temperature-induced phase separation only at 2% (w/w) phosphate, which is approximately fourfold higher than the phosphate concentrations used in growth media for lactic acid bacteria (Planas et al, 1996). The influence of enhanced phosphate concentrations on growth and lactic acid production needs to be established.…”

Section: Discussionmentioning

confidence: 99%

“…The uneven partitioning of cells in an ATPS allows the removal of the product from the cell-free phase. ATPS have been used for the production of bulk chemicals (Drouin and Cooper, 1992;Jarzebski et al, 1992), and specifically for the production of lactic acid (Katzbauer et al, 1995;Kwon et al, 1996;Planas et al, 1996Planas et al, , 1997. However, an even distribution of lactic acid between the two phases, together with the cost of the poly-mers, makes this process concept economically nonviable.…”

Section: Introductionmentioning

confidence: 99%

Novel polymer-polymer conjugates for recovery of lactic acid by aqueous two-phase extraction

Planas

Kozlowski

Harris

et al. 1999

Biotechnol. Bioeng.

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A new family of polymer conjugates is proposed to overcome constraints in the applicability of aqueous two‐phase systems for the recovery of lactic acid. Polyethylene glycol–polyethylenimine (PEI) conjugates and ethylene oxide propylene oxide–PEI (EOPO‐PEI) conjugates were synthesized. Aqueous two‐phase systems were generated when the conjugates were mixed with fractionated dextran or crude hydrolyzed starch. With 2% phosphate buffer in the systems, phase diagrams with critical points of 3.9% EOPO‐PEI–3.8% dextran (DEX) and 3.5% EOPO‐PEI–7.9% crude starch were obtained. The phase separation temperature of 10% EOPO‐PEI solutions titrated with lactic acid to pH 6 was 35°C at 5% phosphate, and increased linearly to 63°C at 2% phosphate. Lactic acid partitioned to the top conjugate‐rich phase of the new aqueous two‐phase systems. In particular, the lactic acid partition coefficient was 2.1 in 10% EOPO‐PEI–8% DEX systems containing 2% phosphate. In the same systems, the partitioning of the lactic acid bacterium, Lactococcus lactis subsp. lactis, was 0.45. The partitioning of propionic, succinic, and citric acids was also determined in the new aqueous two‐phase systems. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 66: 211–218, 1999.

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Enhanced production of lactic acid through the use of a novel aqueous two-phase system as an extractive fermentation system

Cited by 36 publications

References 16 publications

Ionic Liquids Toxicity—Benefits and Threats

Ionic Liquids Toxicity—Benefits and Threats

Analysis of phase composition in aqueous two-phase systems using a two-column chromatographic method: Application to lactic acid production by extractive fermentation

Novel polymer-polymer conjugates for recovery of lactic acid by aqueous two-phase extraction

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