Enhanced production of target bioactive metabolites produced by Pseudomonas aeruginosa LV strain

Bedoya, Juan Carlos; Dealis, Mickely Liuti; Silva, C.S.; Niekawa, Erika Tyemi Goya; Navarro, Miguel Octavio Pérez; Simionato, Ane Stéfano; Modolon, Flúvio; Chryssafidis, Andréas Lazaros; Andrade, Galdino

doi:10.1016/j.bcab.2018.12.024

Cited by 9 publications

(8 citation statements)

References 52 publications

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“…M18 at 28 °C, meaning that, depending on the temperature, the ratio between PCA and PYO will be different [ 23 ]. Similar results were achieved by Bedoya et al [ 27 ] for the production of PCA and PCN by P. aeruginosa LV, where at 28 °C is possible to achieve a higher PCN production, than at 32 °C, favoring PCA production. On the other hand, a considerable decrease in PCN production was observed when the temperature decreased from 21 ºC to 16 °C.…”

Section: Phenazine Productionsupporting

confidence: 89%

“…P. aeruginosa can produce pyocyanin (PYO), PCA, PCN, 1-hydroxyphenazine (1-OH-PHZ), and Aeruginosin A and B [ 5 , 23 ]. Examples of these are P. aeruginosa PA1201 and M18 which produces PCA, like strain LV which, additionally, produces PCN, and P. aeruginosa JY21 which produces PYO [ 23 , 24 , 25 , 26 , 27 , 28 ]. P. fluorescens , strains fp-5 and 2-79, produce only PCA [ 11 , 29 , 30 ].…”

Section: Phenazine-producing Strainsmentioning

confidence: 99%

“…Nutrients such as oxygen, ferric iron, phosphate, sulphate, and magnesium were also reported to be significant in phenazine production [ 22 , 27 , 28 , 64 ]. Low concentrations of ferric iron, phosphate, and sulphate, as well as high magnesium and oxygen levels (above 1%), were found to reduce PCN levels in P. chlororaphis PCL1391 [ 22 ].…”

Section: Phenazine Productionmentioning

confidence: 99%

“…Organic solvents such as ethyl acetate, dichloromethane, chloroform, methylene dichloride, toluene, or diethyl ether are the most reported for this step of the purification process. Then, the organic phase where phenazines are dissolved can be evaporated and the dry solids dissolved in acetonitrile or methanol [ 19 , 20 , 22 , 23 , 27 , 29 , 32 , 52 , 68 , 69 , 70 ]. Some authors, after solvent utilization, include a chromatography step with silica gel to enhance the purification [ 29 , 71 , 72 , 73 , 74 ].…”

Section: Extraction and Purification Of Phenazinesmentioning

confidence: 99%

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Recent Developments in the Biological Activities, Bioproduction, and Applications of Pseudomonas spp. Phenazines

et al. 2023

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Phenazines are a large group of heterocyclic nitrogen-containing compounds with demonstrated insecticidal, antimicrobial, antiparasitic, and anticancer activities. These natural compounds are synthesized by several microorganisms originating from diverse habitats, including marine and terrestrial sources. The most well-studied producers belong to the Pseudomonas genus, which has been extensively investigated over the years for its ability to synthesize phenazines. This review is focused on the research performed on pseudomonads’ phenazines in recent years. Their biosynthetic pathways, mechanism of regulation, production processes, bioactivities, and applications are revised in this manuscript.

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Section: Phenazine Productionsupporting

confidence: 89%

Section: Phenazine-producing Strainsmentioning

confidence: 99%

Section: Phenazine Productionmentioning

confidence: 99%

Section: Extraction and Purification Of Phenazinesmentioning

confidence: 99%

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Recent Developments in the Biological Activities, Bioproduction, and Applications of Pseudomonas spp. Phenazines

et al. 2023

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“…In this study, the antifungal effect of the dichloromethane fraction (F4a) obtained from the cell-free supernatant of P. aeruginosa LV strain cultured under copper stress [22,46] was analyzed on planktonic cells of C. auris CBS 10913 and C. auris CBS 12766 (thereafter, these strains were named CBS 10913 and CBS 12766, respectively). CBS 10913 was sensitive to fluconazole and amphotericin B, whereas CBS 12766 was resistant to both antifungal agents [47].…”

Section: Resultsmentioning

confidence: 99%

Synergistic Antifungal Interaction between Pseudomonas aeruginosa LV Strain Metabolites and Biogenic Silver Nanoparticles against Candida auris

Spoladori,

Andriani,

Castro

et al. 2023

Antibiotics

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Candida auris has been found to be a persistent colonizer of human skin and a successful pathogen capable of causing potentially fatal infection, especially in immunocompromised individuals. This fungal species is usually resistant to most antifungal agents and has the ability to form biofilms on different surfaces, representing a significant therapeutic challenge. Herein, the effect of metabolites of Pseudomonas aeruginosa LV strain, alone and combined with biologically synthesized silver nanoparticles (bioAgNP), was evaluated in planktonic and sessile (biofilm) cells of C. auris. First, the minimal inhibitory and fungicidal concentration values of 3.12 and 6.25 µg/mL, respectively, were determined for F4a, a semi-purified bacterial fraction. Fluopsin C and indolin-3-one seem to be the active components of F4a. Like the semi-purified fraction, they showed a time- and dose-dependent fungicidal activity. F4a and bioAgNP caused severe changes in the morphology and ultrastructure of fungal cells. F4a and indolin-3-one combined with bioAgNP exhibited synergistic fungicidal activity against planktonic cells. F4a, alone or combined with bioAgNP, also caused a significant decrease in the number of viable cells within the biofilms. No cytotoxicity to mammalian cells was detected for bacterial metabolites combined with bioAgNP at synergistic concentrations that presented antifungal activity. These results indicate the potential of F4a combined with bioAgNP as a new strategy for controlling C. auris infections.

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Bacterial antagonists and their cell-free cultures efficiently suppress canker disease in citrus lime

et al. 2019

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Enhanced production of target bioactive metabolites produced by Pseudomonas aeruginosa LV strain

Cited by 9 publications

References 52 publications

Recent Developments in the Biological Activities, Bioproduction, and Applications of Pseudomonas spp. Phenazines

Recent Developments in the Biological Activities, Bioproduction, and Applications of Pseudomonas spp. Phenazines

Synergistic Antifungal Interaction between Pseudomonas aeruginosa LV Strain Metabolites and Biogenic Silver Nanoparticles against Candida auris

Bacterial antagonists and their cell-free cultures efficiently suppress canker disease in citrus lime

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