Enhanced production of target bioactive metabolites produced by Pseudomonas Aeruginosa LV strain

Bedoya, Juan Carlos; Dealis, Mickely Liuti; Silva, C.S.; Niekawa, Erika Tyemi Goya; Navarro, Miguel Octavio Pérez; Simionato, Ane Stéfano; Modolon, Flúvio; Chryssafidis, Andréas Lazaros; Andrade, Galdino

doi:10.1016/j.bcab.2019.01.025

Cited by 6 publications

(2 citation statements)

References 52 publications

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“…Fluopsin C production used a patented method (Patent PI0803350-1 – INPI 09/12/20092008) 1 , with some modifications (Bedoya et al, 2019). Briefly, P. aeruginosa LV strain was cultured in nutrient broth supplied with 5 mg/L of cooper chloride.…”

Section: Methodsmentioning

confidence: 99%

Fluopsin C for Treating Multidrug-Resistant Infections: In vitro Activity Against Clinically Important Strains and in vivo Efficacy Against Carbapenemase-Producing Klebsiella pneumoniae

et al. 2019

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The increasing emergence of multidrug-resistant (MDR) organisms in hospital infections is causing a global public health crisis. The development of drugs with effective antibiotic action against such agents is of the highest priority. In the present study, the action of Fluopsin C against MDR clinical isolates was evaluated under in vitro and in vivo conditions. Fluopsin C was produced in cell suspension culture of Pseudomonas aeruginosa LV strain, purified by liquid adsorption chromatography and identified by mass spectrometric analysis. Bioactivity, bacterial resistance development risk against clinically important pathogenic strains and toxicity in mammalian cell were initially determined by in vitro models. In vivo toxicity was evaluated in Tenebrio molitor larvae and mice. The therapeutic efficacy of intravenous Fluopsin C administration was evaluated in a murine model of Klebsiella pneumoniae (KPC) acute sepsis, using six different treatments. The in vitro results indicated MIC and MBC below 2 μg/mL and low bacterial resistance development frequency. Electron microscopy showed that Fluopsin C may have altered the exopolysaccharide matrix and caused disruption of the cell wall of MDR bacteria. Best therapeutic results were achieved in mice treated with a single dose of 2 mg/kg and in mice treated with two doses of 1 mg/kg, 8 h apart. Furthermore, acute and chronic histopathological studies demonstrated absent nephrotoxicity and moderate hepatotoxicity. The results demonstrated the efficacy of Fluopsin C against MDR organisms in in vitro and in vivo models, and hence it can be a novel therapeutic agent for the control of severe MDR infections.

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Section: Methodsmentioning

confidence: 99%

Fluopsin C for Treating Multidrug-Resistant Infections: In vitro Activity Against Clinically Important Strains and in vivo Efficacy Against Carbapenemase-Producing Klebsiella pneumoniae

et al. 2019

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“…The F4A fraction and Fluopsin C used in this study were obtained according to procedures described by Munhoz et al (2017) and Navarro et al (2019), respectively. The F4A contains basically four compounds (mg/L): phenazine-1-carboxylic acid (14 mg), phenazine-carboxamide (9 mg), indol-3-one (1 mg), and Fluopsin C (6 mg) (Bedoya et al, 2019). IC 90 was used in the following assays for enhancing the analyses.…”

Section: Effect Of F4a Fraction and Fluopsin C On The Ultra-structure Of Gram-negative And Gram-positive Bacteriamentioning

confidence: 99%

Determining the Targets of Fluopsin C Action on Gram-Negative and Gram-Positive Bacteria

et al. 2020

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The antibiotic activity of metalloantibiotic compounds has been evaluated since the 90s, and many different modes of action were characterized. In the last decade, the effects of secondary metabolites produced by Pseudomonas aeruginosa LV strain, including a cupric compound identified as Fluopsin C, were tested against many pathogenic bacteria strains, proving their high antibiotic activity. In the present study, the bactericidal mechanisms of action of Fluopsin C and the semi-purified fraction F4A were elucidated. The results found in electron microscopy [scanning electron microscopy (SEM) and transmission electronic microscopy (TEM)] demonstrated that both Fluopsin C and F4A are affecting the cytoplasmatic membrane of Gram-positive and Gram-negative bacteria. These results were confirmed by fluorescence microscopy, where these bacteria presented permeabilization of their cytoplasmatic membranes after contact with the semi-purified fraction and pure compound. Using electronic and fluorescence microscopy, along with bacterial mutant strains with marked divisional septum, the membrane was defined as the primary target of Fluopsin C in the tested bacteria.

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The two faces of pyocyanin - why and how to steer its production?

Jabłońska

Augustyniak

Dubrowska

et al. 2023

World J Microbiol Biotechnol

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The ambiguous nature of pyocyanin was noted quite early after its discovery. This substance is a recognized Pseudomonas aeruginosa virulence factor that causes problems in cystic fibrosis, wound healing, and microbiologically induced corrosion. However, it can also be a potent chemical with potential use in a wide variety of technologies and applications, e.g. green energy production in microbial fuel cells, biocontrol in agriculture, therapy in medicine, or environmental protection. In this mini-review, we shortly describe the properties of pyocyanin, its role in the physiology of Pseudomonas and show the ever-growing interest in it. We also summarize the possible ways of modulating pyocyanin production. We underline different approaches of the researchers that aim either at lowering or increasing pyocyanin production by using different culturing methods, chemical additives, physical factors (e.g. electromagnetic field), or genetic engineering techniques. The review aims to present the ambiguous character of pyocyanin, underline its potential, and signalize the possible further research directions.

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Enhanced production of target bioactive metabolites produced by Pseudomonas Aeruginosa LV strain

Cited by 6 publications

References 52 publications

Fluopsin C for Treating Multidrug-Resistant Infections: In vitro Activity Against Clinically Important Strains and in vivo Efficacy Against Carbapenemase-Producing Klebsiella pneumoniae

Fluopsin C for Treating Multidrug-Resistant Infections: In vitro Activity Against Clinically Important Strains and in vivo Efficacy Against Carbapenemase-Producing Klebsiella pneumoniae

Determining the Targets of Fluopsin C Action on Gram-Negative and Gram-Positive Bacteria

The two faces of pyocyanin - why and how to steer its production?

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