Enhanced production of thermostable lipase from Bacillus cereus ASSCRC-P1 in waste frying oil based medium using statistical experimental design

Abstract

This study aim to isolate, identify a bacterial isolate and optimize production medium using frying oil waste for lipase production. Nine strains were isolated from an Egyptian soil samples. Among the isolates, a potent bacterial candidate ASSCRC-P1 was found to be the most potent lipase producer strain at 60 °C. Genotypic identification of ASSCRC-P1 showed 94% similarity with Bacillus sp. strains. Phylogenetic tree confirmed that ASSCRC-P1 was nearly similar to Bacillus cereus. Therefore, it was given the name Bacillus cereus ASSCRC-P1 and its 16S rRNA nucleotide has been deposited in the GenBank Data Library under the accession number: KJ531440. A sequential optimization strategy, based on statistical experimental designs, was employed to enhance the lipase production by this strain. A 2-level Plackett-Burman design was applied to differentiate between the bioprocess parameters that significantly influence lipase production followed by Box-Behnken design to optimize the amounts of variables which have the highest positive significant effect on lipase production. Overall more than 2.15-fold improvement in lipase production was achieved due to optimization compared to that obtained using the basal medium.

Optimization of culture conditions for enhanced lipase production by an indigenousBacillus aryabhattaiSE3-PB using response surface methodology

“…They are found in different habitats, including industrial wastes, vegetable oil processing industries, dairy industries and oil-contaminated sites, among others [18]. Lipase production from many species of Bacillus, including Bacillus pumilus [19], Bacillus subtilis [20], Bacillus thermoleovorans [21], Bacillus cereus [22], Bacillus coagulans [23], Bacillus megaterium [24] and Bacillus licheniformis [25], has been reported. However, this is the first report on production and optimization of lipase from Bacillus aryabhattai.…”

Determination of Desirable Attributes of an Indigenous Burkholderia Isolate Towards Biological Control of Plant Pathogenic Fungi and Its Microbial Enzyme Production

“…PCR cycle conditions included 35 cycles of denaturing step at 94 o C for 45 sec, annealing step at 50.2 o C for 45 sec and an extension step at 72 o C for 1 min. Expected size of the PCR product was approximately 1500 bp (Awad et al, 2015). PCR products were analyzed on 1.5 % agarose gel and amplicons having expected molecular size were sequenced at Asiri Diagnostic Services (Pvt) Ltd and subjected to DNA homology search using BLAST, NCBI.…”

Two Novel Clusters of Nocardiopsis Recovered From Haloalkaline Environment: Potential Resource of Multi-Advantageous Lipases

“…Moreover, Lipase could be used for the treatment of the malignant tumor basing on its role as an activator of tumor necrosis factor (Kato et al, 1989). Lipases are applied in detergent and laundry industries basing on their ability to remove fat stains (Awad, et al, 2015;Schmi, and Verger, 1998). In paper industries, lipases were applied in the treatment of lumber to be processed to lowgrade paper (Schmid, and d Verger, 1998).…”