Enhanced Production of Vitamin K2 from Bacillus subtilis (natto) by Mutation and Optimization of the Fermentation Medium

Song, Junying; Liu, Hongxia; Wang, Li; Dai, Jun; Liu, Yan; Liu, Hui; Zhao, Genhai; Wang, Peng; Zheng, Z. P.

doi:10.1590/s1982-88372014000100019

Cited by 13 publications

(23 citation statements)

References 15 publications

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“…According to monitoring studies using 200 µg/mL IONs@APTES, production of MK–7 started after 24 h, and reached its maximum of 37.36 mg/L after 108 h. The results are in agreement with previous studies [ 21 , 22 ] indicating that the production of MK–7 is partly growth-associated. Time course fermentation studies showed that the pH of the medium decreased from 7.19 to 6.34 during the first 12 h, and increased gradually to 8.18 after 48 h. This increase in pH might be a result of the production of ammonia during the course of fermentation [ 23 ].…”

Section: Resultssupporting

confidence: 90%

Impact of 3–Aminopropyltriethoxysilane-Coated Iron Oxide Nanoparticles on Menaquinone-7 Production Using B. subtilis

et al. 2017

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One of the major issues associated with industrial production of menaquinone-7 (MK–7) is the low fermentation yield. In this study, we investigated the effect of iron oxide nanoparticles coated with 3–aminopropyltriethoxysilane (IONs@APTES) on the production of MK–7 using B. subtilis (ATCC 6633). Decoration of B. subtilis cells with IONs@APTES significantly enhanced both MK–7 production and yield. An approximately two-fold increase in MK–7 production (41 mg/L) was observed in the presence of 500 µg/mL IONs@APTES, as compared to MK–7 production using untreated bacteria (22 mg/L). This paper, therefore, illustrates the immense biotechnological potential of IONs@APTES in increasing MK–7 concentration using B. subtilis, and its future role in bioprocess engineering.

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Section: Resultssupporting

confidence: 90%

Impact of 3–Aminopropyltriethoxysilane-Coated Iron Oxide Nanoparticles on Menaquinone-7 Production Using B. subtilis

et al. 2017

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“…Particularly, Bacillus subtilis natto has been shown to produce a range of vitamin K2 homologues (MK-4, MK-5, MK-6, MK-7, and MK-8) with the major component being MK7 and accounting for more than 90% of total vitamin K2 production [157]. Depending on the strategy employed, Bacillus subtilis natto has been reported to produce MK-7 with yields of 3.6 mg/L in a mutant strain resistant to 1-hydroxy-2-naphthoic acid (HNA) [158], 32.2 mg/L in an isolated strain from the traditional Japanese food natto fermented for 72 h [159], 35.0 mg/L in a menadione-resistant mutant strain isolated from natto cultivated for four days [160], and 1719 µg/100 g natto [161] in a multiple resistant mutant strain. More recently, approaches of metabolic engineering to enhance MK-7 production in Bacillus subtilis have been reported [162].…”

Section: Vitamin K2mentioning

confidence: 99%

Vitamin K as a Diet Supplement with Impact in Human Health: Current Evidence in Age-Related Diseases

et al. 2020

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Vitamin K health benefits have been recently widely shown to extend beyond blood homeostasis and implicated in chronic low-grade inflammatory diseases such as cardiovascular disease, osteoarthritis, dementia, cognitive impairment, mobility disability, and frailty. Novel and more efficient nutritional and therapeutic options are urgently needed to lower the burden and the associated health care costs of these age-related diseases. Naturally occurring vitamin K comprise the phylloquinone (vitamin K1), and a series of menaquinones broadly designated as vitamin K2 that differ in source, absorption rates, tissue distribution, bioavailability, and target activity. Although vitamin K1 and K2 sources are mainly dietary, consumer preference for diet supplements is growing, especially when derived from marine resources. The aim of this review is to update the reader regarding the specific contribution and effect of each K1 and K2 vitamers in human health, identify potential methods for its sustainable and cost-efficient production, and novel natural sources of vitamin K and formulations to improve absorption and bioavailability. This new information will contribute to foster the use of vitamin K as a health-promoting supplement, which meets the increasing consumer demand. Simultaneously, relevant information on the clinical context and direct health consequences of vitamin K deficiency focusing in aging and age-related diseases will be discussed.

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“…Since maize contains not only large amounts of starch (about 70%-75%, dry basis), protein (about 10%), and lipid (about 4%-5%) but also multi-vitamins (vitamin A, vitamin B 1 , vitamin B 2 , etc. ), maize is a promising feedstock for organic compound production by microbial fermentation, such as lactic acid and amino acid (Song et al, 2014;Xu et al, 2014). However, as far as we know there has been no research on MK-7 production from maize meal hydrolysate.…”

Section: Introductionmentioning

confidence: 99%

Menaquinone-7 production from maize meal hydrolysate by Bacillus isolates with diphenylamine and analogue resistance

Zhang

2017

J. Zhejiang Univ. Sci. B

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A menaquinone-7 (MK-7) high-producing strain needs to be isolated to increase MK-7 production, in order to meet a requirement of MK-7 given the low MK-7 content in food products. This article focuses on developing MK-7 high-producing strains via screening and mutagenesis by an atmospheric and room temperature plasma (ARTP) mutation breeding system. We isolated an MK-7-producing strain Y-2 and identified it as Bacillus amyloliquefaciens, which produced (7.1±0.5) mg/L of MK-7 with maize meal hydrolysate as carbon source. Then, an MK-7 high-producing strain B. amyloliquefaciens H.β.D.R.-5 with resistance to 1-hydroxy-2-naphthoic acid, β-2-thienylalanine, and diphenylamine was obtained from the mutation of the strain Y-2 using an ARTP mutation breeding system. Using strain H.β.D.R.-5, efficient production of MK-7 was achieved ((30.2±2.7) mg/L). In addition, the effects of nitrogen sources, prenyl alcohols, and MgSO on MK-7 production were investigated, suggesting that soymeal extract combined with yeast extract, isopentenol, and MgSO was beneficial. Under the optimized condition, the MK-7 production and biomass-specific yield reached (61.3±5.2) mg/L and 2.59 mg/L per OD unit respectively in a 7-L fermenter. These results demonstrated that strain H.β.D.R.-5 has the capacity to produce MK-7 from maize meal hydrolysate, which could reduce the substrate cost.

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Enhanced Production of Vitamin K2 from Bacillus subtilis (natto) by Mutation and Optimization of the Fermentation Medium

Abstract: The aim of this study was to enhance the production of vitamin K 2 by using N-methyl-N-nitro-N-nitroso-guanidine (NTG)

Cited by 13 publications

References 15 publications

Impact of 3–Aminopropyltriethoxysilane-Coated Iron Oxide Nanoparticles on Menaquinone-7 Production Using B. subtilis

Impact of 3–Aminopropyltriethoxysilane-Coated Iron Oxide Nanoparticles on Menaquinone-7 Production Using B. subtilis

Vitamin K as a Diet Supplement with Impact in Human Health: Current Evidence in Age-Related Diseases

Menaquinone-7 production from maize meal hydrolysate by Bacillus isolates with diphenylamine and analogue resistance

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