Enhanced production of xylanase enzyme by Fusariummoniliforme using submerged fermentation

Abstract

Hemicellulose degrading enzymes play an important role in bioconversion of agro and agro-industrial wastes. In this study, production of hemicellulase by six fungal isolates was determined under submerged culture using corn cobs xylan as a carbon source and enzyme inducer at different incubation periods. The results indicated that Alternariatenuisshowed the lowest enzyme productivity (156.95 ± 2.07U/l)while the highest enzyme production (2,594.44 ± 62.25U/l) was found by Fusariummoniliforme. One-factor-at-atime (OFAT) revealed maximum enzyme productivity of 10,950.11 ± 98.45 U/l at; corn cobs xylan (6g/l), yeast extract (4g/l), inorganic salts (MgSO 4 , KH 2 PO 4 , CaCl 2 , FeSO 4 , and MnSO 4 ), initial pH (5), initial inoculum size (4%), 150 rpm and temperature (30℃ ).

Bioprocessos: Processos Fermentativos Para Produção De Enzimas E Imobilização Para Aplicação Industrial

“…Já o estudo realizado por HUSSEIN et al (2018). Foi feita a produção de enzimas hemicelulolíticas através seis fungos isolados diferentes empregando a (FS), que consistiu na utilização de xilana de sabugo de milho como fonte de carbono e na estimulação da produção enzimática em diferentes períodos da incubação.…”

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“…The oligosaccharide DP 4 was used as carbon sources for growing the three probiotics and (in parallel) a pathogenic strain E. coli. A medium-containing blank made out of uninoculated mannooligosaccharides (Hussein et al, 2010). E. coli was cultured on nutritional broth medium, while probiotics were produced on conventional MRS broth medium.…”