Enhanced production of β-glucuronidase from Penicillium purpurogenum Li-3 by optimizing fermentation and downstream processes

Huang, Shuxian; Feng, Xudong; Li, Chun

doi:10.1007/s11705-015-1544-0

Cited by 9 publications

(6 citation statements)

References 29 publications

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“…To overcome the bottleneck encountered, new breakthroughs need to be found. Some studies suggested that enzymes derived from microbes functioned well in engineered microbes (Huang et al 2015;Wang et al 2019;Xu et al 2018). Considering the long-term harmony and coevolution in plant-endophyte symbioses, endophytes are realized to be potential alternatives for terpenoid bioproduction, either directly or indirectly (Venugopalan and Srivastava 2015).…”

Section: Introductionmentioning

confidence: 99%

Endophytes: the novel sources for plant terpenoid biosynthesis

Chen

Xing

et al. 2021

Appl Microbiol Biotechnol

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Terpenoids are natural compounds predominantly present in plants. They have many pharmaceutical and/or nutritional functions, and have been widely applied in medical, food, and cosmetics industries. Recently, terpenoids have been used in the clinical treatment of COVID-19 due to the good antiviral activities. The increasing demand for terpenoids in international markets poses a serious threat to many plant species. For environmentally sustainable development, microbial cell factories have been utilized as the promising platform to produce terpenoids. Nevertheless, the bioproduction of most terpenoids cannot meet commercial requirements due to the low cost-benefit ratio until now. The biosynthetic potential of endophytes has gained attention in recent decades owing to the continual discovery of endophytes capable of synthesizing plant bioactive compounds. Accordingly, endophytes could be alternative sources of terpenoid-producing strains or terpenoid synthetic genes. In this review, we summarized the research progress describing the main and supporting roles of endophytes in terpenoid biosynthesis and biotransformation, and discussed the current problems and challenges which may prevent the further exploitation. This review will improve our understanding of endophyte resources for terpenoid production in industry in the future. Key points • The mechanisms employed by endophytes in terpenoid synthesis in vivo and in vitro.• Endophytes have the commercial potentials in terpenoid bioproduction and biotransformation.• Synthetic biology and multiomics will improve terpenoid bioproduction in engineered cell factories.

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Section: Introductionmentioning

confidence: 99%

Endophytes: the novel sources for plant terpenoid biosynthesis

Chen

Xing

et al. 2021

Appl Microbiol Biotechnol

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“…During the second stage of the fermentation, GL was added as an inducer, and the cells began to utilize GL and release a large quantity of β‐GUS, resulting in a rapid increase in GAMG production. This strategy increased the biomass by 1.5 times and the enzyme activity by 5.4 times compared to that of cultivation with GL as the sole carbon source (Huang, Feng, & Li, ). Thus, the above described fed‐batch strategy allows efficient industrial production of GAMG with significantly improved control of the nutrient concentration, lower carbon catabolite repression, increased biomass yield, and higher enzyme productivity.…”

Section: Biotechnological Production Of Gamgmentioning

confidence: 99%

“…β‐GUS was originally used as a specific marker, allowing for the rapid detection of E. coli in water and other environmental samples (Wildeboer, Amirat, Price, & Abuknesha, ). Recently, its importance in the hydrolysis of one or two units of glucuronide acid of the endogenous and exogenous glycosyl compound to generate various high‐value derivatives such as GAMG has been recognized and exploited (Amin et al., ; Huang et al., ; Kaleem et al., ).…”

Section: β‐Glucuronidasementioning

confidence: 99%

Glycyrrhetic Acid 3‐O‐Mono‐β‐d‐glucuronide (GAMG): An Innovative High‐Potency Sweetener with Improved Biological Activities

Guo

Katiyo

et al. 2018

Comp Rev Food Sci Food Safe

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Glycyrrhetic acid 3-O-mono-β-d-glucuronide (GAMG) is an important derivative of glycyrrhizin (GL) and has attracted considerable attention, especially in the food and pharmaceutical industries, due to its natural high sweetness and strong biological activities. The biotransformation process is becoming an efficient route for GAMG production with the advantages of mild reaction conditions, environmentally friendly process, and high production efficiency. Recent studies showed that several β-glucuronidases (β-GUS) are key GAMG-producing enzymes, displaying a high potential to convert GL directly into the more valuable GAMG and providing new insights into the generation of high-value compounds. This review provides details of the structural properties, health benefits, and potential applications of GAMG. The progress in the development of the biotransformation processes and fermentation strategies to improve the yield of GAMG is also discussed. This work further summarizes recent advances in the enzymatic synthesis of GAMG using β-GUS with emphasis on the physicochemical and biological properties, molecular modifications, and enzymatic strategies to improve β-GUS biocatalytic efficiencies. This information contributes to a better framework to explore production and application of bioactive GAMG.

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“…Eventually, HPH can effectively reduce the size of natural fibers, producing nanofibers from cellulose and chitin [16,17]. The cellulose or chitin suspensions are usually subjected to multiple passes (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30) through the homogenizer at pressures ranging from 100 to 150 MPa [17], and the nanofiber sizes obtained are dependent on the number of passes and pressure [18]. Since cricket powder contains chitin, the impact of HPH on this constituent is also very important.…”

Section: Introductionmentioning

confidence: 99%

Effect of Combined High-Pressure Homogenization and Biotechnological Processes on Chitin, Protein, and Antioxidant Activity of Cricket Powder-Based Ingredients

Rossi,

Gottardi,

Barbiroli

et al. 2024

Foods

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The main objective of this work was to evaluate the combined effect of a biotechnology process, based on selected yeast strains, and a high-pressure homogenization (HPH) treatment on the microbiological quality, structural organization of proteins, chitin content, and antioxidant activity of a mixture of cricket powder (Acheta domesticus) and water. Compared to untreated samples, the cricket matrix treated with HPH four times at 180 MPa promoted the growth of the inoculated Yarrowia lipolytica and Debaryomyces hansenii strains. HPH did not affect the concentration of chitin; however, the combination with microorganisms tended to reduce the content. Although the antioxidant activity increased from 0.52 to 0.68 TAC mM/TE after a 48 h incubation in the control, it was further improved by the combination of HPH and D. hansenii metabolism, reaching a value of 0.77 TAC mM/TE. The combination of the two approaches also promoted a reduction in the intensity of bands with molecular weights between 31 and 21.5 kDa in favor of bands with a lower molecular weight. In addition, HPH treatment reduced the number of accessible thiols, suggesting protein structure changes that may further impact the technological properties of cricket powder.

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Enhanced production of β-glucuronidase from Penicillium purpurogenum Li-3 by optimizing fermentation and downstream processes

Cited by 9 publications

References 29 publications

Endophytes: the novel sources for plant terpenoid biosynthesis

Endophytes: the novel sources for plant terpenoid biosynthesis

Glycyrrhetic Acid 3‐O‐Mono‐β‐d‐glucuronide (GAMG): An Innovative High‐Potency Sweetener with Improved Biological Activities

Effect of Combined High-Pressure Homogenization and Biotechnological Processes on Chitin, Protein, and Antioxidant Activity of Cricket Powder-Based Ingredients

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