2018
DOI: 10.1021/acssuschemeng.8b04359
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Enhanced Protein Extraction from Oilseed Cakes Using Glycerol–Choline Chloride Deep Eutectic Solvents: A Biorefinery Approach

Abstract: The enhanced extraction of proteins from rapeseed cake (RC) and evening primrose cake (EC) using a glycerol−choline chloride deep eutectic solvent (DES, glyceline) is reported. Protein-rich precipitates were obtained by adding water (antisolvent) to the DES extract derived at different processing temperatures. The presence of proteins in precipitates has been confirmed by several techniques, such as NMR, ATR-IR, TGA, CHN, and SDS-PAGE. Yield of precipitates improved with increasing temperature of treatment, re… Show more

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Cited by 86 publications
(58 citation statements)
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“…Natural deep eutectic solvents, NADESs, which meet green chemistry objectives and are composed of naturally occurring substances from cellular metabolites [9], are considered to be suitable alternatives for organic solvents, ILs, and even for common DESs [10]. Among a diverse list of applications [4,11,12,13,14,15,16,17,18,19], the use of DESs and NADESs in biofuel [8,20,21,22] and bio-oil [23,24] production, as reaction media or extractive agents [25,26,27,28,29,30,31,32,33,34,35], and as media to tune intermolecular interactions [36] are of special importance to researchers.…”
Section: Introductionmentioning
confidence: 99%
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“…Natural deep eutectic solvents, NADESs, which meet green chemistry objectives and are composed of naturally occurring substances from cellular metabolites [9], are considered to be suitable alternatives for organic solvents, ILs, and even for common DESs [10]. Among a diverse list of applications [4,11,12,13,14,15,16,17,18,19], the use of DESs and NADESs in biofuel [8,20,21,22] and bio-oil [23,24] production, as reaction media or extractive agents [25,26,27,28,29,30,31,32,33,34,35], and as media to tune intermolecular interactions [36] are of special importance to researchers.…”
Section: Introductionmentioning
confidence: 99%
“…On the other hand, the agri-food industry produces large quantities of byproducts that are abandoned and can cause potential environmental issues. Meanwhile, these byproducts could be a remarkable source of valuable compounds like phenolic compounds [25], proteins [27], flavonoids [29], anthocyanins [33], lignin [26], peptides [31], polyphenolic antioxidants [49], chitin [50], etc. Therefore, particular interest has been given to food residue and biomass resources, to solve the environmental issues related to waste product management and to valorize these resources to produce green fuels and platform chemicals.…”
Section: Introductionmentioning
confidence: 99%
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“…Though gram-scale use of DES in LLE or LSE is appropriate for food analysis process, scale-up use of DES will be highly desirable for various food and by-product process. DESs composed of ChCl and glycerol were successfully used for protein extraction from oilseed (i.e., rapeseed) cake [52]. In this work, 45 g DES were utilized for treatment of a 5 g sample (oilseed cake) and the reuse of DES was also conducted, indicating the potential large-scale applications of DESs.…”
Section: General Considerations Using Dess As Extraction Solventsmentioning
confidence: 99%
“…Importantly, a high recovery was found using these DESs comparison with 60% (v/v) ethanol-water solvent. Moreover, enhanced protein extraction from oilseed cakes (e.g., rapeseed) was developed using DES composed of glycerol-ChCl [52]. The protein-rich precipitates could be obtained by adding water as anti-solvent to the DES (glycerol-ChCl 2:1 M ratio) extract derived at 60 • C.…”
Section: Dess For the Extraction Proteinmentioning
confidence: 99%