Enhanced recognition of a modified peptide antigen by cytotoxic T cells specific for influenza nucleoprotein

A pentapeptide as minimal antigenic determinant for MHC class I-restricted T lymphocytes

“…The data refer to a one-hour incubation of target cells with peptide. Differences in antigenic potency are also refiected by the time needed for optimal target formation; five minutes is long enough to prepare an optimal nonapeptide target, which is in line with data for a high-affinity peptide 24 , whereas plateau lysis could be achieved with the pentapeptide only when the peptide pulse was prolonged to two hours or more (not shown). Because clone lEI does not discriminate between nonapeptide and pentapeptide target once the target is formed, the limiting step is apparently the association between the peptide ligand and its MHC receptor on the target-cell surface.…”

Binding of alanine‐substituted peptides to the MHC class I protein, Kd

“…The original peptide, NP 147-155, is a Kd-restricted peptide from the influenza virus nucleoprotein [17] and has a sequence (TYQRTRALV) consistent with the consensus motif identified for Kd. The other seven peptides synthesized had each of the residues replaced separately by an Ala. Positions 7 and 9 were not tested because the original amino acids are Ala and Val, respectively.…”

Intranasal lipopeptide primes lung‐resident memory CD8+ T cells for long‐term pulmonary protection against influenza

“…The I-E d -restricted Th epitope, sequence ALNNRFQIKGVELKS, was derived from the light chain of the influenza haemagglutinin and elicits CD4 + T cells that are cross-reactive with all H3 influenza viruses [23]. The H-2K d -restricted CTL epitope NP 147-155 , sequence TYQR-TRALV, was derived from the nucleoprotein of the virus and is common to all type A influenza strains [24,25]. The Th peptide and the CTL peptide were separated in sequence by a single lysine residue.…”