Enhanced Recovery of Fastidious Organisms from Urine Culture in the Setting of Total Laboratory Automation

Lainhart, William; Burnham, Carey‐Ann D.

doi:10.1128/jcm.00546-18

Cited by 22 publications

(13 citation statements)

References 15 publications

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“…Growth of these slow-growing organisms is likely due to the enhanced recovery of microorganisms using smart incubators. A recent study in 2018 evaluated recovery of organisms from urine culture pre-and postautomation (22). Interestingly, the use of smart incubators allowed for routine detection of Neisseria gonorrhoeae from blood agar plates more often than did standard incubation.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of WASPLab Software To Automatically Read chromID CPS Elite Agar for Reporting of Urine Cultures

et al. 2019

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Urine cultures are among the most common specimens received by clinical laboratories and generate a major share of the laboratory workload. Chromogenic agar can expedite culture results, but technologist review is still needed. In this study, we evaluated the ability of the WASPLab software to interpret urine specimens plated onto chromID CPS Elite (CPSE) agar. Urine specimens submitted for bacterial culture were plated onto CPSE agar with a 1-μl loop using the WASP. Each plate was imaged after 0 and 18 h of incubation, and colonies were enumerated by color using the WASPLab software and a technologist’s reading from a high-definition (HD) monitor. The results were reported as negative if <10 colonies/plate were detected. Laboratory information system (LIS) time stamps were used to measure the time to result. A total of 1,581 urine cultures were tested. The sensitivity and specificity of the software were 99.8% and 68.5%, respectively, which included 2 manual-positive/automation-negative (MP/AN) results and 170 manual-negative/automation-positive (MN/AP) results. Of the 170 MN/AP specimens, 116 were caused by microcolonies missed by the technologist. The remaining MN/AP results were caused by either count differences near the 10-colony threshold (n = 43) or count differences of >50 CFU (n = 11). The use of both CPSE agar and the WASPLab software improved the time to result for urine culture, reducing the average time to result by 4 h 42 min for negative specimens and 3 h 28 min for positive specimens compared to that with standard-of-care testing. These data demonstrate that the use of CPSE agar and automated plate reading has the potential to improve turnaround time while maintaining high sensitivity and reducing urine culture workload.

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Section: Discussionmentioning

confidence: 99%

Evaluation of WASPLab Software To Automatically Read chromID CPS Elite Agar for Reporting of Urine Cultures

et al. 2019

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“…The system has to ensure the production of high-quality images for the detection of bacterial growth and to differentiate colony morphotypes at the earliest stages of incubation (33). Imaging technology has the ability to detect bacterial growth earlier, particularly for slow-growing bacteria (24,34,35). It is also a powerful tool to differentiate and track bacterial morphotypes, such as those for P. aeruginosa mucoid or small-colony variants in cystic fibrosis patients' sputum ( Fig.…”

Section: Is Total Laboratory Automation Already Practical and Mature?mentioning

confidence: 99%

Consolidation of Clinical Microbiology Laboratories and Introduction of Transformative Technologies

et al. 2020

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SUMMARY Clinical microbiology is experiencing revolutionary advances in the deployment of molecular, genome sequencing-based, and mass spectrometry-driven detection, identification, and characterization assays. Laboratory automation and the linkage of information systems for big(ger) data management, including artificial intelligence (AI) approaches, also are being introduced. The initial optimism associated with these developments has now entered a more reality-driven phase of reflection on the significant challenges, complexities, and health care benefits posed by these innovations. With this in mind, the ongoing process of clinical laboratory consolidation, covering large geographical regions, represents an opportunity for the efficient and cost-effective introduction of new laboratory technologies and improvements in translational research and development. This will further define and generate the mandatory infrastructure used in validation and implementation of newer high-throughput diagnostic approaches. Effective, structured access to large numbers of well-documented biobanked biological materials from networked laboratories will release countless opportunities for clinical and scientific infectious disease research and will generate positive health care impacts. We describe why consolidation of clinical microbiology laboratories will generate quality benefits for many, if not most, aspects of the services separate institutions already provided individually. We also define the important role of innovative and large-scale diagnostic platforms. Such platforms lend themselves particularly well to computational (AI)-driven genomics and bioinformatics applications. These and other diagnostic innovations will allow for better infectious disease detection, surveillance, and prevention with novel translational research and optimized (diagnostic) product and service development opportunities as key results.

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“…First, the detection rate of all bacteria and especially of fastidious species rose. This effect was most pronounced for Alloscardovia omnicolens , Actinotignum schaalii , Gardnerella vaginalis and Neisseria gonorrhoeae among others [32,33].…”

Section: Workflowmentioning

confidence: 99%

Laboratory Automation in Clinical Microbiology

Burckhardt

2018

Bioengineering

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Laboratory automation is currently the main organizational challenge for microbiologists. Automating classic workflows is a strenuous process for the laboratory personnel and a huge and long-lasting financial investment. The investments are rewarded through increases in quality and shortened time to report. However, the benefits for an individual laboratory can only be estimated after the implementation and depending on the classic workflows currently performed. The two main components of automation are hardware and workflow. This review focusses on the workflow aspects of automation and describes some of the main developments during recent years. Additionally, it tries to define some terms which are related to automation and specifies some developments which would further improve automated systems.

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Enhanced Recovery of Fastidious Organisms from Urine Culture in the Setting of Total Laboratory Automation

Cited by 22 publications

References 15 publications

Evaluation of WASPLab Software To Automatically Read chromID CPS Elite Agar for Reporting of Urine Cultures

Evaluation of WASPLab Software To Automatically Read chromID CPS Elite Agar for Reporting of Urine Cultures

Consolidation of Clinical Microbiology Laboratories and Introduction of Transformative Technologies

Laboratory Automation in Clinical Microbiology

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