Enhanced regeneration in spinal cord injury by concomitant treatment with granulocyte colony-stimulating factor and neuronal stem cells

Pan, Hung-Chuan; Cheng, Fu‐Chou; Lai, Shu-Zhen; Yang, Dar-Yu; Wang, Yeou-Chih; Lee, Maw‐Sheng

doi:10.1016/j.jocn.2007.03.020

Cited by 42 publications

(48 citation statements)

References 40 publications

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“…Spinal cord transection [9][10][11]14,21,[23][24][25][26]28,31,32 was the most common injury model followed by hemisection. 12,13,[15][16][17][18][19]22,27,29,30 Most studies contained information about the effects of acute intervention after induction of SCI but some studies also contained information about the effects in the sub-acute or chronic phase.…”

Section: Overall Characteristics Of Published Studiesmentioning

confidence: 99%

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Biomaterial-based interventions for neuronal regeneration and functional recovery in rodent model of spinal cord injury: A systematic review

Krishna

Konakondla

Nicholas

et al. 2013

The Journal of Spinal Cord Medicine

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Context: There is considerable interest in translating laboratory advances in neuronal regeneration following spinal cord injury (SCI). A multimodality approach has been advocated for successful functional neuronal regeneration. With this goal in mind several biomaterials have been employed as neuronal bridges either to support cellular transplants, to release neurotrophic factors, or to do both. A systematic review of this literature is lacking. Such a review may provide insight to strategies with a high potential for further investigation and potential clinical application. Objective: To systematically review the design strategies and outcomes after biomaterial-based multimodal interventions for neuronal regeneration in rodent SCI model. To analyse functional outcomes after implantation of biomaterial-based multimodal interventions and to identify predictors of functional outcomes. Methods: A broad PubMed, CINHAL, and a manual search of relevant literature databases yielded data from 24 publications; 14 of these articles included functional outcome information. Studies reporting behavioral data in rat model of SCI and employing biodegradable polymer-based multimodal intervention were included. For behavioral recovery, studies using severe injury models (transection or severe clip compression (>16.9 g) or contusion (50 g/cm)) were categorized separately from those investigating partial injury models (hemisection or moderate-to-severe clip compression or contusion). Results: The cumulative mean improvements in Basso, Beattie, and Bresnahan scores after biomaterial-based interventions are 5.93 (95% CI = 2.41 − 9.45) and 4.44 (95% CI = 2.65 -6.24) for transection and hemisection models, respectively. Factors associated with improved outcomes include the type of polymer used and a follow-up period greater than 6 weeks. Conclusion: The functional improvement after implantation of biopolymer-based multimodal implants is modest. The relationship with neuronal regeneration and functional outcome, the effects of inflammation at the site of injury, the prolonged survival of supporting cells, the differentiation of stem cells, the effective delivery of neurotrophic factors, and longer follow-up periods are all topics for future elucidation. Future investigations should strive to further define specific factors associated with improved functional outcomes in clinically relevant models.

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Section: Overall Characteristics Of Published Studiesmentioning

confidence: 99%

“…used, followed by fibrin. 15,17,25 Most of the studies described various scaffold designs whereas some utilized gel preparations of biopolymers. 18,25,27,31 Linearlyoriented scaffolds were investigated in 12 studies with a pore size varying from 10 to 660 μm.…”

Section: Implant Characteristicsmentioning

confidence: 99%

Biomaterial-based interventions for neuronal regeneration and functional recovery in rodent model of spinal cord injury: A systematic review

Krishna

Konakondla

Nicholas

et al. 2013

The Journal of Spinal Cord Medicine

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“…The improved survival was accompanied by a rescue of host motoneurons, stabilization of weight and an increase in the size of the muscle fibers. The grafted NPCs were small and round and exhibited no neural markers, suggesting that they remained in an undifferentiated state (Pan et al, 2008). Magnetically labeled NPCs were suspended with activated magnetic beads and individual NPCs, were transplanted to the co-cultures.…”

Section: Non-embryonic Stem Cellsmentioning

confidence: 99%

“…Atalay et al (2007Atalay et al ( , 2008 showed that Nogo-A monoclonal antibodies (NEP1-40) promote functional recoveries in injured rat spinal cords. Pan et al (2008) showed that synergy between Granulocate CSF (G-CSF) and neuronal stem cells may be because of the increased proliferation of progenitor cells in the injured area and increased expression of neuronal stem cell markers extrinsically or intrinsically in the distal end of injured cord. The study provided evidence that lithium may have therapeutic potential in cell replacement strategies for CNS injury because of its ability to promote proliferation and neuronal generation of grafted NPCs and reduce the host immune reaction (Su et al, 2007).…”

Section: Non-embryonic Stem Cellsmentioning

confidence: 99%

Stem Cells: Current Approach and Future Prospects in Spinal Cord Injury Repair

Zhang

Huang²,

Qian

et al. 2010

The Anatomical Record

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Spinal cord injury (SCI) invariably results in the loss of neurons and axonal degeneration at the lesion site, leading to permanent paralysis and loss of sensation. There has been no successful treatment for severe spinal cord injuries to recover back to normal function yet. Studies have shown that the transplantation of stem cells may provide an effective treatment for SCI because of the self-renewing and multipotential nature of these cells. Stem cells have the capability to repair injured nervous tissue through replacement of damaged cells, neuroprotection, or the creation of an environment conducive to regeneration by endogenous cells. Up to today several types of stem cells have been transplanted into the injured spinal cord. However, the question of which cell type is most beneficial for SCI treatment is still unresolved. There are still several limitations to the current data sets which require further investigation. Anat Rec, 293:519-530, 2010. V V C 2009 Wiley-Liss, Inc.

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“…16 G-CSF binds with a specific receptor, G-CSF receptor (G-CSFR) and promotes the proliferation and differentiation of granulocyte hemopoietic progenitor to protect neutrophils from apoptosis. G-CSFR is present within the CNS 17 and increasing evidence has revealed that G-CSF may inhibit neuronal apoptosis associated with neural injury/disease and, in this way, promote neuroprotective effects. [18][19][20] In particular, G-CSF has been shown to exert beneficial effects via an anti-autophagic mechanism in cardiomyopathic hamster models.…”

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confidence: 99%

G-CSF promotes autophagy and reduces neural tissue damage after spinal cord injury in mice

Guo

Liu

Zhang

et al. 2015

Laboratory Investigation

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Granulocyte colony-stimulating factor (G-CSF) was investigated for its capacity to induce autophagy and related neuroprotective mechanisms in an acute spinal cord injury model. To accomplish this goal, we established a mouse spinal cord hemisection model to test the effects of recombinant human G-CSF. The results showed that autophagy was activated after spinal cord injury and G-CSF appears to induce a more rapid activation of autophagy within injured spinal cords as compared with that of non-treated animals. Apoptosis as induced in mechanically injured neurons with G-CSF treatment was enhanced after inhibiting autophagy by 3-methyladenine (3-MA), which partially blocked the neuroprotective effect of autophagy as induced by G-CSF. In addition, G-CSF inhibited the activity of the NF-κB signal pathway in neurons after mechanical injury. We conclude that G-CSF promotes autophagy by inhibiting the NF-κB signal pathway and protects neuronal structure after spinal cord injury. We therefore suggest that G-CSF, which rapidly induces autophagy after spinal cord injury to inhibit neuronal apoptosis, may thus provide an effective auxiliary therapeutic intervention for spinal cord injury.

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Enhanced regeneration in spinal cord injury by concomitant treatment with granulocyte colony-stimulating factor and neuronal stem cells

Cited by 42 publications

References 40 publications

Biomaterial-based interventions for neuronal regeneration and functional recovery in rodent model of spinal cord injury: A systematic review

Biomaterial-based interventions for neuronal regeneration and functional recovery in rodent model of spinal cord injury: A systematic review

Stem Cells: Current Approach and Future Prospects in Spinal Cord Injury Repair

G-CSF promotes autophagy and reduces neural tissue damage after spinal cord injury in mice

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