Enhanced reparative dentinogenesis of biphasic calcium phosphate ceramics containing calcium-deficient hydroxyapatite (CDHA) and strontium-incorporated CDHA in direct pulp capping

Hu, Yun; Wan, Lingyun; Xiao, Yumei; Wang, Yuyi; Wu, Zhiwu; Guo, Weihua; Yang, Hui; Hu, Tao

doi:10.1016/j.mtcomm.2022.104231

Cited by 5 publications

(4 citation statements)

References 49 publications

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“…6,23 The high Ca/P ion ratio in the GIC-CMC10% group (►Table 3) suggests the formation of calcium-deficient HAP crystals with the potential to mature into HAP, enhancing the physical properties of dentin, including elasticity modulus and resistance to caries. 34,35 Different results were observed in the Ca/P ion ratio between the GIC group and the GIC-CMC5% group, indicating a lack of crystal maturation toward hydroxyapatite. 36 The low ratio results occurred due to the insufficient supply of calcium ions by the GIC and GIC-CMC5% as the raw materials for the remineralization process.…”

Section: Discussionmentioning

confidence: 96%

Evaluation of Physical Properties in Carboxymethyl Chitosan Modified Glass Ionomer Cements and the Effect for Dentin Remineralization: SEM/EDX, Compressive Strength, and Ca/P Ratio

Putranto,

Meidyawati,

Dwiseptyoga

et al. 2024

Eur J Dent

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Objective The aim of this article was to evaluate the effects of modifying glass ionomer cement (GIC) with carboxymethyl chitosan (CMC) on surface morphology and remineralization outcomes by examining dentin morphology and calcium ion composition changes. Materials and Methods Thirty holes in a cylindrical acrylic mold were filled with three groups of restorative materials: GIC, GIC modified with CMC (GIC-CMC) 5%, and GIC-CMC10%. The surface morphology of each group's materials was observed using scanning electron microscopy (SEM). The compressive strength measurement was performed using a universal testing machine. The dentin remineralization process was performed by applying GIC, GIC-CMC5%, and GIC-CMC10% materials for 14 days on demineralized dentin cavities treated with 17% ethylenediamine tetraacetic acid (EDTA) for 7 days. A morphological evaluation was conducted using SEM. The calcium ion composition and calcium-to-phosphorous (Ca/P) ratio were examined using an energy-dispersive X-ray (EDX). Statistical Analysis The Kruskal–Wallis and post-hoc Mann–Whitney U tests were performed to compare all four groups of calcium ions (p < 0.05). Results The modification of GIC with CMC affected the morphological changes in the materials in the form of reduced porosity and increased fractures. A significant difference was found in compressive strength between the GIC-CMC modification materials of GIC-CMC5% and GIC-CMC10% and the GIC control group. The dentin tubule morphology and surface changes were observed after applying GIC, GIC-CMC5%, and GIC-CMC10% materials for 14 days, as evaluated by SEM. The EDX examination showed an increase in calcium ion content and hydroxyapatite formation (Ca/P ratio) after applying the GIC-CMC10% material. Conclusion The surface porosity of the GIC modification material with the addition of CMC tended to decrease. However, an increase in cracked surfaces that widened, along with the rise in CMC percentage, was found. This modification also reduced the compressive strength of the materials, with the lowest average yield at 10% CMC addition. Therefore, the modification of GIC with CMC affects changes in morphology, calcium ion composition, and Ca/P ratio in demineralized dentin.

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Section: Discussionmentioning

confidence: 96%

Evaluation of Physical Properties in Carboxymethyl Chitosan Modified Glass Ionomer Cements and the Effect for Dentin Remineralization: SEM/EDX, Compressive Strength, and Ca/P Ratio

Putranto,

Meidyawati,

Dwiseptyoga

et al. 2024

Eur J Dent

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“…These defects are one the possible reasons for DPC failure, as these defects are mainly responsible for the secondary infection of the pulp tissue. Therefore, the thick-uninterrupted MTF or reparative dentine is crucial for maintaining vital pulp after injury (Hu et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Biological evaluation of novel phosphorylated pullulan‐based calcium hydroxide formulations as direct pulp capping materials: An in vivo study on a rat model

Islam,

Liu

et al. 2024

Int Endodontic J

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AimCalcium hydroxide (CH) has been considered as a direct pulp capping materials (DPC) for the last decades despite having some limitations. Phosphorylate pullulan (PPL) incorporated with CH (CHPPL) is a novel biomaterial that was introduced as a promising DPC material. Thus, the aim of the study was to evaluate the inflammatory response and mineralized tissue formation (MTF) ability of PPL‐based CH formulations on rat molars after DPC.MethodologyThis study consisted of six groups: CH with 1% PPL (CHPPL‐1); 3% PPL (CHPPL‐3); 5% PPL (CHPPL‐5); Dycal and NEX MTA Cement (N‐MTA) as the positive control, and no capping materials (NC). One hundred twenty maxillary first molar cavities were prepared on Wistar rats. After capping, all the cavities were restored with 4‐META/MMA‐TBB resin and pulpal responses were evaluated at days 1, 7, and 28. Kruskal–Wallis followed by Mann–Whitney U‐test was performed with a significance level of 0.05. Immunohistochemical expression of IL‐6, Nestin, and DMP‐1 was observed.ResultsAt day 1, CHPPL‐1, N‐MTA, and Dycal exhibited no to mild inflammation, whilst CHPPL‐3, CHPPL‐5, and NC showed mild to moderate inflammation, and the results were significantly different (p < .05). At day 7, mild to moderate inflammation was observed in CHPPL‐1, N‐MTA, and Dycal, whereas CHPPL‐3, CHPPL‐5, and NC exhibited moderate to severe inflammation. Significant differences were observed between CHPPL‐1 and N‐MTA with NC (p < .05), CHPPL‐1 and CHPPL‐3 with CHPPL‐5 and Dycal (p < .05), and CHPPL‐3 with N‐MTA (p < .05). A thin layer of mineralized tissue formation (MTF) was observed in all groups. At day 28, CHPPL‐1, Dycal, and N‐MTA showed no to mild inflammation, whilst CHPPL‐3, CHPPL‐5, and NC exhibited mild to severe inflammation, and statistically significant difference was detected (p < .05). CHPPL‐1, Dycal, and N‐MTA exhibited continuous MTF, whilst CHPPL‐3, CHPPL‐5, and NC had thicker and interrupted MTF. Significant differences were observed between CHPPL‐1, CHPPL‐3, and N‐MTA with NC group (p < .05). Variable expressions of IL‐6, Nestin, and DMP‐1 indicated differences in the materials' impact on odontoblast‐like cell formation and tissue mineralization.ConclusionsThese findings suggest that CHPPL‐1 has the potential to minimize pulpal inflammation and promote MTF and had similar efficacy as MTA cement.

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“…Later, they were maintained in standard medium, which was low glucose Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA), penicillin (100 units/mL), and streptomycin (100 mg/mL) at 37 °C in 5% CO 2 . Cells were sub-cultured when they reached 80% confluence and used at passages 3∼6 ( Hu et al, 2022 ). Osteogenic differentiation was induced by osteogenic induction medium (OIM), the standard medium supplemented with 10 mM b-glycerophosphate, 50 mg/mL ascorbic acid, and 100 nM dexamethasone (Sigma–Aldrich, St. Louis, MO, USA) for 1, 3, 5, and 7 days ( Chen et al, 2013 ).…”

Section: Methodsmentioning

confidence: 99%

Metabolic shift and the effect of mitochondrial respiration on the osteogenic differentiation of dental pulp stem cells

Wan

Wang

Cheng

et al. 2023

PeerJ

Self Cite

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Background Metabolism shifts from glycolysis to mitochondrial oxidative phosphorylation are vital during the differentiation of stem cells. Mitochondria have a direct function in differentiation. However, the metabolic shift and the effect of mitochondria in regulating the osteogenic differentiation of human dental pulp stem cells (hDPSCs) remain unclear. Methods Human dental pulp stem cells were collected from five healthy donors. Osteogenic differentiation was induced by osteogenic induction medium. The activities of alkaline phosphatase, hexokinase, pyruvate kinase, and lactate dehydrogenase were analyzed by enzymatic activity kits. The extracellular acidification rate and the mitochondrial oxygen consumption rate were measured. The mRNA levels of COL-1, ALP, TFAM, and NRF1 were analyzed. The protein levels of p-AMPK and AMPK were detected by western blotting. Results Glycolysis decreased after a slight increase, while mitochondrial oxidative phosphorylation continued to increase when cells grew in osteogenic induction medium. Therefore, the metabolism of differentiating cells switched to mitochondrial respiration. Next, inhibiting mitochondrial respiration with carbonyl cyanide-chlorophenylhydrazone, a mitochondrial uncoupler inhibited hDPSCs differentiation with less ALP activity and decreased ALP and COL-1 mRNA expression. Furthermore, mitochondrial uncoupling led to AMPK activation. 5-Aminoimidazole-4-carboxamide ribonucleotide, an AMPK activator, simulated the effect of mitochondrial uncoupling by inhibiting osteogenic differentiation, mitochondrial biogenesis, and mitochondrial morphology. Mitochondrial uncoupling and activation of AMPK depressed mitochondrial oxidative phosphorylation and inhibited differentiation, suggesting that they may serve as regulators to halt osteogenic differentiation from impaired mitochondrial oxidative phosphorylation.

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Enhanced reparative dentinogenesis of biphasic calcium phosphate ceramics containing calcium-deficient hydroxyapatite (CDHA) and strontium-incorporated CDHA in direct pulp capping

Cited by 5 publications

References 49 publications

Evaluation of Physical Properties in Carboxymethyl Chitosan Modified Glass Ionomer Cements and the Effect for Dentin Remineralization: SEM/EDX, Compressive Strength, and Ca/P Ratio

Evaluation of Physical Properties in Carboxymethyl Chitosan Modified Glass Ionomer Cements and the Effect for Dentin Remineralization: SEM/EDX, Compressive Strength, and Ca/P Ratio

Biological evaluation of novel phosphorylated pullulan‐based calcium hydroxide formulations as direct pulp capping materials: An in vivo study on a rat model

Metabolic shift and the effect of mitochondrial respiration on the osteogenic differentiation of dental pulp stem cells

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