Enhanced Resistance to <i>Cucumber mosaic virus</i> in the <i>Arabidopsis thaliana ssi2</i> Mutant Is Mediated via an SA-Independent Mechanism

Sekine, Ken Taro; Nandi, Ashis Kumar; Ishihara, T.; Hase, S.; Ikegami, Machiko; Shah, Jyoti; Takahashi, Hideki

doi:10.1094/mpmi.2004.17.6.623

Cited by 54 publications

(29 citation statements)

References 59 publications

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“…To quantify the systemic spread of virus in CMV-infected plants, the coat protein of CMV was detected immunologically by a modified tissue-printing method (Takahashi et al 2002;Sekine et al 2004). To quantify the amount of virus multiplication, the amount of viral coat protein was measured by the ELISA method as described previously ).…”

Section: Virus Inoculation and Detectionmentioning

confidence: 99%

Single amino acid alterations in Arabidopsis thaliana RCY1 compromise resistance to Cucumber mosaic virus, but differentially suppress hypersensitive response-like cell death

et al. 2006

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Resistance to an yellow strain of Cucumber mosaic virus [CMV(Y)] in Arabidopsis thaliana ecotype C24 is conferred by the CC-NBS-LRR type R gene, RCY1. RCY1-conferred resistance is accompanied by a hypersensitive response (HR), which is characterized by the development of necrotic local lesion (NLL) at the site of infection that restricts viral spread. To further characterize the role of RCY1 in NLL formation we have identified six recessive CMV(Y)-susceptible rcy1 mutants, four of which contain single amino acid substitutions in RCY1: rcy1-2 contains a D to N substitution in the CC domain, rcy1-3 and rcy1-4 contain R to K and E to K substitutions, respectively, in the LRR domain, and rcy1-6 contains a W to C substitution in the NBS domain. The rcy1-5 and rcy1-7 contain nonsense mutations in the LRR and NBS domains, respectively. Although the virus systemically spread in all six rcy1 mutants, HR-associated cell death was differentially induced in these mutants. In comparison to the wild type C24 plant, HR was not observed in the CMV(Y)-inoculated leaves of the rcy1-3, rcy1-5, rcy1-6 and rcy1-7 mutants. In contrast, delayed NLL development was observed in the virus inoculated leaves of the rcy1-2 and rcy1-4 mutants. In addition, necrosis accompanied by elevated accumulation of PR gene transcript also appeared in the non-inoculated leaves of the rcy1-2 and rcy1-4 mutants. Trans-complementation was observed between the rcy1-2 and rcy1-4 alleles; in F1 plants derived from a cross between rcy1-2 and rcy1-4, HR associated cell death was accelerated and systemic spread of the virus was partially suppressed than in the homozygous rcy1-2 and rcy1-4 plants. Our results suggest that the CC, NBS and LRR domains of RCY1 are required for restriction of virus spread but differentially impact the induction of HR-like cell death. Furthermore, these results also predict inter-molecular interaction involving RCY1 in Arabidopsis resistance to CMV(Y).

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Section: Virus Inoculation and Detectionmentioning

confidence: 99%

Single amino acid alterations in Arabidopsis thaliana RCY1 compromise resistance to Cucumber mosaic virus, but differentially suppress hypersensitive response-like cell death

et al. 2006

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“…Arabidopsis ssi2 mutant, which accumulates high levels of plant defense hormone salicylic acid (SA), confers resistance to CMV (Sekine et al, 2004). Based on the experimental evidences, Sekine et al (2004) demonstrated that the resistance to CMV in ssi2 mutant is unrelated to SA production and the dwarf phenotype. Some Arabidopsis mutants related to the defense hormone ethylene, such as acs6 mutant, also shows resistance to YoMV (Chen et al, 2013).…”

Section: Promising Genetic Resources For Recessive Resistancementioning

confidence: 99%

“…Some Arabidopsis mutants related to the defense hormone ethylene, such as acs6 mutant, also shows resistance to YoMV (Chen et al, 2013). Although the loss-of-susceptibility of the mutants related to defense responses may be due to elevated antiviral defense signaling(s), mutants such as ssi2 mutant frequently show an abnormal growth phenotype (Sekine et al, 2004). Remarkably, a triple mutant of homeodomain-leucine zipper protein 1 ( HAT1 ) and its related genes HAT2 and HAT3 confers loss-of-susceptibility to CMV without any growth defect despite the high level of SA and JA accumulation (Zou et al, 2016).…”

Section: Promising Genetic Resources For Recessive Resistancementioning

confidence: 99%

Recessive Resistance to Plant Viruses: Potential Resistance Genes Beyond Translation Initiation Factors

et al. 2016

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The ability of plant viruses to propagate their genomes in host cells depends on many host factors. In the absence of an agrochemical that specifically targets plant viral infection cycles, one of the most effective methods for controlling viral diseases in plants is taking advantage of the host plant’s resistance machinery. Recessive resistance is conferred by a recessive gene mutation that encodes a host factor critical for viral infection. It is a branch of the resistance machinery and, as an inherited characteristic, is very durable. Moreover, recessive resistance may be acquired by a deficiency in a negative regulator of plant defense responses, possibly due to the autoactivation of defense signaling. Eukaryotic translation initiation factor (eIF) 4E and eIF4G and their isoforms are the most widely exploited recessive resistance genes in several crop species, and they are effective against a subset of viral species. However, the establishment of efficient, recessive resistance-type antiviral control strategies against a wider range of plant viral diseases requires genetic resources other than eIF4Es. In this review, we focus on recent advances related to antiviral recessive resistance genes evaluated in model plants and several crop species. We also address the roles of next-generation sequencing and genome editing technologies in improving plant genetic resources for recessive resistance-based antiviral breeding in various crop species.

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“…The Arabidopsis ssi2 mutant, which was identified as a suppressor of the npr1 mutant 22 , is defective in a gene encoding a stearoyl-acyl carrier protein (ACP) desaturase 13 . The ssi2 plants accumulate endogenous SA at high levels; they constitutively express PR1 and PR2 and exhibit enhanced resistance to multiple pathogens 13,21 . However, the dependence of the ssi2 defense phenotypes on the SA pathway remains unclear, because the defense phenotypes appear even in SA-deficient nahG-overexpressing plants, albeit not prominently 22 .…”

Section: Other Factors That Influence the Sa Signaling Pathway In Ricementioning

confidence: 99%

Salicylic Acid Signaling Pathway in Rice and the Potential Applications of Its Regulators

Takatsuji

Jiang

Sugano

2010

JARQ

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The salicylic acid (SA) signaling pathway plays a crucial role in systemic acquired resistance in dicots. Several chemical inducers, which are also called "plant activators," such as benzothiadiazole (BTH), protect plants from diseases by acting on the SA signaling pathway. Several studies by us and other groups have revealed that rice also has the SA signaling pathway that shares signaling components with the SA signaling pathway in dicots. OsNPR1 is a rice counterpart of NPR1, a transcriptional coactivator that plays a central role in the SA pathway in Arabidopsis. OsWRKY45 is a BTH-inducible rice-specific transcription factor. Knockdown experiments have demonstrated that both these transcriptional regulators are essential for BTH-induced blast resistance in rice. Unlike the SA pathway in Arabidopsis, wherein most of the SA/BTH-regulated genes are controlled by NPR1, the pathway in rice branches into OsNPR1-and OsWRKY45-dependent pathways. OsWRKY45 overexpression confers very high resistance to blast and leaf blight diseases in rice, and it causes only minor growth retardation through the "priming effect"-an action mechanism characteristic of plant activators. OsWRKY45 is a potential target for genetic manipulation of rice in order to confer broad-spectrum disease resistance; however, it is necessary to improve the construct to drive OsWRKY45 expression in rice in order to optimize the growth and disease resistance of the plants. Here, we have reviewed recent advances in the studies on the SA pathway in rice, with particular focus on the potential practical applications of the signaling components.

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Enhanced Resistance to Cucumber mosaic virus in the Arabidopsis thaliana ssi2 Mutant Is Mediated via an SA-Independent Mechanism

Cited by 54 publications

References 59 publications

Single amino acid alterations in Arabidopsis thaliana RCY1 compromise resistance to Cucumber mosaic virus, but differentially suppress hypersensitive response-like cell death

Single amino acid alterations in Arabidopsis thaliana RCY1 compromise resistance to Cucumber mosaic virus, but differentially suppress hypersensitive response-like cell death

Recessive Resistance to Plant Viruses: Potential Resistance Genes Beyond Translation Initiation Factors

Salicylic Acid Signaling Pathway in Rice and the Potential Applications of Its Regulators

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