Enhanced resolution in MAS NMR for combinatorial chemistry

Shapiro, Michael J.; Chin, Jefferson; Martí, Roger; Jarosinski, Mark A.

doi:10.1016/s0040-4039(97)00092-0

Cited by 55 publications

(20 citation statements)

References 8 publications

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“…This technique evaluates the consumption of double bonds upon crosslinking and is thus a measure for the efficiency of crosslinking. (Sandra Van Vlierberghe José C. Martins, and Peter Dubruel, 2010) Conventional 1 H-NMR spectroscopy does not enable the characterization of crosslinked polymer networks due to the considerable line broadening which results from the presence of dipolar couplings and magnetic susceptibility effects (Ramadhar, Amador, Ditty, & Power, 2008;Shapiro, Chin, Marti, & Jarosinski, 1997). HR-MAS spectroscopy circumvents this line broadening by rapidly rotating the sample at a magic angle of 54.7° with respect to the static magnetic field, following swelling of the material (Ramadhar et al, 2008).…”

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confidence: 99%

Gelatin- and starch-based hydrogels. Part A: Hydrogel development, characterization and coating

Nieuwenhove

Salamon

Peters

et al. 2016

Carbohydrate Polymers

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The present work aims at constructing the ideal scaffold matrix of which the physico-chemical properties can be altered according to the targeted tissue regeneration application. Ideally, this scaffold should resemble the natural extracellular matrix (ECM) as close as possible both in terms of chemical composition and mechanical properties. Therefore, hydrogel films were developed consisting of methacrylamide-modified gelatin and starch-pentenoate building blocks because the ECM can be considered as a crosslinked hydrogel network consisting of both polysaccharides and structural, signaling and cell-adhesive proteins. For the gelatin hydrogels, three different substitution degrees were evaluated including 31%, 72% and 95%. A substitution degree of 32% was applied for the starch-pentenoate building block. Pure gelatin hydrogels films as well as interpenetrating networks with gelatin and starch were developed. Subsequently, these films were characterized using gel fraction and swelling experiments, high resolution-magic angle spinning (1)H NMR spectroscopy, rheology, infrared mapping and atomic force microscopy. The results indicate that both the mechanical properties and the swelling extent of the developed hydrogel films can be controlled by varying the chemical composition and the degree of substitution of the methacrylamide-modified gelatin applied. The storage moduli of the developed materials ranged between 14 and 63kPa. Phase separation was observed for the IPNs for which separated starch domains could be distinguished located in the surrounding gelatin matrix. Furthermore, we evaluated the affinity of aggrecan for gelatin by atomic force microscopy and radiolabeling experiments. We found that aggrecan can be applied as a bioactive coating for gelatin hydrogels by a straightforward physisorption procedure. Thus, we achieved distinct fine-tuning of the physico-chemical properties of these hydrogels which render them promising candidates for tissue engineering approaches.

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mentioning

confidence: 99%

Gelatin- and starch-based hydrogels. Part A: Hydrogel development, characterization and coating

Nieuwenhove

Salamon

Peters

et al. 2016

Carbohydrate Polymers

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show abstract

“…The domain of application ranges from the study of organic molecules and peptides bound to a swollen solid resin support (4)(5)(6)(7)(8)(9)(10)(11) to polymers, lipids (12), and human and animal tissues (13,14).…”

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confidence: 99%

Destruction of Magnetization during TOCSY Experiments Performed under Magic Angle Spinning: Effect of Radial B1 Inhomogeneities

Piotto

Bourdonneau

Furrer

et al. 2001

Journal of Magnetic Resonance

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“…The domain of application of this new technique ranges from the study of organic molecules and peptides bound to a solid resin support (3)(4)(5)(6)(7)(8)(9)(10) to polymers, lipids (11), and human and animal tissues (12,13).…”

mentioning

confidence: 99%

Origin of the Residual NMR Linewidth of a Peptide Bound to a Resin under Magic Angle Spinning

Elbayed

Bourdonneau

Furrer

et al. 1999

Journal of Magnetic Resonance

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Enhanced resolution in MAS NMR for combinatorial chemistry

Cited by 55 publications

References 8 publications

Gelatin- and starch-based hydrogels. Part A: Hydrogel development, characterization and coating

Gelatin- and starch-based hydrogels. Part A: Hydrogel development, characterization and coating

Destruction of Magnetization during TOCSY Experiments Performed under Magic Angle Spinning: Effect of Radial B1 Inhomogeneities

Origin of the Residual NMR Linewidth of a Peptide Bound to a Resin under Magic Angle Spinning

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