Enhanced rheological behaviors of alginate hydrogels with carrageenan for extrusion-based bioprinting

Kim, Myoung Hwan; Lee, Yong Wook; Jung, Won‐Kyo; Oh, Junghwan; Nam, Seung Yun

doi:10.1016/j.jmbbm.2019.06.014

Cited by 142 publications

(107 citation statements)

References 54 publications

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“…Thixotropic properties of the polymers which relate to the ability of a material to recover after the application of shear, are also important during PAM or FDM extrusion. Ideally, the polymer should be able to recover completely to its original state after a shear force is applied during extrusion, and as quickly as possible [125]. Recovery time is also important so that when the polymer is deposited on the printing platform, it can be given the time it needs to recover before any subsequent layers are added [77].…”

Section: Polymers Rheology and Its Impact On Structure And Processmentioning

confidence: 99%

Polymers for Extrusion-Based 3D Printing of Pharmaceuticals: A Holistic Materials–Process Perspective

et al. 2020

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Three dimensional (3D) printing as an advanced manufacturing technology is progressing to be established in the pharmaceutical industry to overcome the traditional manufacturing regime of 'one size fits for all'. Using 3D printing, it is possible to design and develop complex dosage forms that can be suitable for tuning drug release. Polymers are the key materials that are necessary for 3D printing. Among all 3D printing processes, extrusion-based (both fused deposition modeling (FDM) and pressure-assisted microsyringe (PAM)) 3D printing is well researched for pharmaceutical manufacturing. It is important to understand which polymers are suitable for extrusion-based 3D printing of pharmaceuticals and how their properties, as well as the behavior of polymer–active pharmaceutical ingredient (API) combinations, impact the printing process. Especially, understanding the rheology of the polymer and API–polymer mixtures is necessary for successful 3D printing of dosage forms or printed structures. This review has summarized a holistic materials–process perspective for polymers on extrusion-based 3D printing. The main focus herein will be both FDM and PAM 3D printing processes. It elaborates the discussion on the comparison of 3D printing with the traditional direct compression process, the necessity of rheology, and the characterization techniques required for the printed structure, drug, and excipients. The current technological challenges, regulatory aspects, and the direction toward which the technology is moving, especially for personalized pharmaceuticals and multi-drug printing, are also briefly discussed.

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Section: Polymers Rheology and Its Impact On Structure And Processmentioning

confidence: 99%

Polymers for Extrusion-Based 3D Printing of Pharmaceuticals: A Holistic Materials–Process Perspective

et al. 2020

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“…Briefly, cell-loadable hydrogels require shear thinning properties to allow for extrusion through a print head and nozzle. 46 Ideally, the hydrogel ink solution would be viscous enough to allow for storage/loading in a print cartridge without leaking and preventing cell sedimentation. Exact figures vary, but it is generally accepted that a bioink should display viscosity values in the range of 30 mPa·s (lower limit) to 25 × 10 3 mPa·s (upper limit) to be considered suitable for printing.…”

Section: Bioprinting Techniquesmentioning

confidence: 99%

“…As the material is extruded, viscosity should increase as the shear force is removed, allowing for control over flow of the material and maximizing resolution. 19 , 23 , 46 − 48 Traditionally, gelation would be subsequently triggered, allowing for preservation of the printed structure. This is, however, often difficult to achieve when using hydrogel precursor solutions of low viscosity.…”

Section: Bioprinting Techniquesmentioning

confidence: 99%

Emulating Human Tissues and Organs: A Bioprinting Perspective Toward Personalized Medicine

et al. 2020

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The lack of in vitro tissue and organ models capable of mimicking human physiology severely hinders the development and clinical translation of therapies and drugs with higher in vivo efficacy. Bioprinting allow us to fill this gap and generate 3D tissue analogues with complex functional and structural organization through the precise spatial positioning of multiple materials and cells. In this review, we report the latest developments in terms of bioprinting technologies for the manufacturing of cellular constructs with particular emphasis on material extrusion, jetting, and vat photopolymerization. We then describe the different base polymers employed in the formulation of bioinks for bioprinting and examine the strategies used to tailor their properties according to both processability and tissue maturation requirements. By relating function to organization in human development, we examine the potential of pluripotent stem cells in the context of bioprinting toward a new generation of tissue models for personalized medicine. We also highlight the most relevant attempts to engineer artificial models for the study of human organogenesis, disease, and drug screening. Finally, we discuss the most pressing challenges, opportunities, and future prospects in the field of bioprinting for tissue engineering (TE) and regenerative medicine (RM).

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“…For extrusion, hydrogels need to be prepared such that the viscoelastic properties and shear modulus during extrusion is appropriate for proper printing of features at high fidelity and resolution. Approaches include tuning the degree of crosslinking of the bioink and formation of composites or mixtures such as with NiCu NPs [237], e-polylysine [238], carrageenan [239], gelatin [240][241][242], and nanocellulose [243,244]. Rheological studies show composition ratio, printing temperature, extrusion pressure, and crosslinking concentration affect fidelity and resolution [239,240,242,245].…”

Section: Process and Materialsmentioning

confidence: 99%

“…Inclusion of NiCu [237], e-polylysine [238], carrageenan [239], gelatin [240][241][242], cellulose [243,244], PVA [252], TiO 2 [253], β-TCP [253]; Feature size 150 µm, E: 280 kPa [240] Myoblasts [242]; endothelial cells [259]; E.coli [260], growth factor [250], human adipose stem cells [248]; human induced pluripotent stem cells [261]; chondrocytes [243,244,251]; Schwann cells [265] Cellulose (4.3.4) Mixed with alginate [244,[266][267][268][269][270][271][272]; As reinforcement material, E: 2.5-22.5 kPa [281] Feature size 500 µm [280]; Tensile E: 0.67-0.63 GPa [282] Chondrocytes for cartilage tissue engineering [244,269,271]; human induced pluripotent stem cells, bone-marrow hMSCs [272]; pancreatic cancer cells [268]; fibroblast and hepatoma cells [270]; NIH 3T3 cells [274] Collagen (4.4.1)…”

Section: ) Applicationsmentioning

confidence: 99%

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

2019

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The realization of biomimetic microenvironments for cell biology applications such as organ-on-chip, in vitro drug screening, and tissue engineering is one of the most fascinating research areas in the field of bioengineering. The continuous evolution of additive manufacturing techniques provides the tools to engineer these architectures at different scales. Moreover, it is now possible to tailor their biomechanical and topological properties while taking inspiration from the characteristics of the extracellular matrix, the three-dimensional scaffold in which cells proliferate, migrate, and differentiate. In such context, there is therefore a continuous quest for synthetic and nature-derived composite materials that must hold biocompatible, biodegradable, bioactive features and also be compatible with the envisioned fabrication strategy. The structure of the current review is intended to provide to both micro-engineers and cell biologists a comparative overview of the characteristics, advantages, and drawbacks of the major 3D printing techniques, the most promising biomaterials candidates, and the trade-offs that must be considered in order to replicate the properties of natural microenvironments.

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Enhanced rheological behaviors of alginate hydrogels with carrageenan for extrusion-based bioprinting

Cited by 142 publications

References 54 publications

Polymers for Extrusion-Based 3D Printing of Pharmaceuticals: A Holistic Materials–Process Perspective

Polymers for Extrusion-Based 3D Printing of Pharmaceuticals: A Holistic Materials–Process Perspective

Emulating Human Tissues and Organs: A Bioprinting Perspective Toward Personalized Medicine

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

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