Enhanced saccharification of reed and rice straws by the addition of β-1,3-1,4-glucanase with broad substrate specificity and calcium ion

Kim, Dong Uk; Kim, Hee Jung; Jeong, Younhee; Na, Han Beur; Cha, Young-Lok; Koo, Bon‐Sung; Kim, Jungho; Yun, Han Dae; Lee, Jung-Kul; Kim, Hoon

doi:10.1007/s13765-015-0013-2

Cited by 9 publications

(4 citation statements)

References 25 publications

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“…Substrate specificities for pure β-1,4-glucan (CMC) and mixed-linked β-1,3-1,4-glucans (barley β-glucan or lichenan) and for small substrates probably contributed to observed differences between reed and rice straw powders. It has been previously reported saccharification efficiencies were increased by using pretreated materials [43], an optimized crude enzyme cocktail [44], and an ionic liquid-tolerant cocktail for an ionic liquid-pretreated biomass [45]. In addition, Cel5L-p50 or Cel5L-p35 could be used as additives to improve the saccharification of lignocellulosic substrates, because they both enhance degree of saccharification of pretreated biomasses, regardless of CBM_3 deletion.…”

Section: A C C E P T E Dmentioning

confidence: 99%

Roles of Carbohydrate-Binding Module (CBM) of an Endo-��-1,4-glucanase (Cel5L) from Bacillus sp. KD1014 in Thermostability and Small-Substrate Hydrolyzing Activity

Lee¹,

Shin²,

Cho³

et al. 2018

Journal of Microbiology and Biotechnology

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An endo-β-1,4-glucanase gene, cel5L, was cloned using the shotgun method from Bacillus sp.. The gene, which contained a predicted signal peptide, encoded a protein of 496 amino acid residues, and the molecular mass of the mature Cel5L was estimated to be 51.8 kDa. Cel5L contained a catalytic domain of glycoside hydrolase (GH) family and a carbohydrate-binding module family 3 (CBM_3). Chromatography using HiTrap Q and CHT-II resulted in the isolation of two truncated forms corresponding to 50 (Cel5L-p50) and 35 kDa (Cel5L-p35, CBM_3-deleted form). Both enzymes were optimally active at pH 4.5 and 55°C, but had different half-lives of 4.0 and 22.8 min, respectively, at 70°C. The relative activities of Cel5L-p50 and Cel5L-p35 for barley β-glucan were 377.0 and 246.7%, respectively, compared to those for carboxymethyl-cellulose. The affinity and hydrolysis rate of pNPC by Cel5L-p35 were 1.7 and 3.3 times higher, respectively, than those by Cel5L-p50. Additions of each to a commercial enzyme set increased saccharification of pretreated rice straw powder by 17.5 and 21.0%, respectively. These results suggest CBM_3 is significantly contributing to thermostability, and to affinity and substrate specificity for small substrates, and that these two enzymes could be used as additives to enhance enzymatic saccharification.

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Section: A C C E P T E Dmentioning

confidence: 99%

Roles of Carbohydrate-Binding Module (CBM) of an Endo-��-1,4-glucanase (Cel5L) from Bacillus sp. KD1014 in Thermostability and Small-Substrate Hydrolyzing Activity

Lee¹,

Shin²,

Cho³

et al. 2018

Journal of Microbiology and Biotechnology

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“…In the industrial production of ethanol, endo-β-1,4-glucanase is an important enzyme to saccharify lignocellulosic biomass since it degrades long cellulose chains into shorter chains to facilitate subsequent hydrolysis. It is reported that using BGlc8H with extensive substrate specificity has improved the saccharification of pretreated reed and rice straw [ 44 ]. As a result, the saccharification effects of pretreated biomass with CelA257 will also be further investigated.…”

Section: Resultsmentioning

confidence: 99%

Biochemical Characterization of Novel GH6 Endoglucanase from Myxococcus sp. B6-1 and Its Effects on Agricultural Straws Saccharification

Huang

Dai

et al. 2023

Foods

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Cellulase has been widely used in many industrial fields, such as feed and food industry, because it can hydrolyze cellulose to oligosaccharides with a lower degree of polymerization. Endo-β-1,4-glucanase is a critical speed-limiting cellulase in the saccharification process. In this study, endo-β-1,4-glucanase gene (CelA257) from Myxococcus sp. B6-1 was cloned and expressed in Escherichia coli. CelA257 contained carbohydrate-binding module (CBM) 4-9 and glycosyl hydrolase (GH) family 6 domain that shares 54.7% identity with endoglucanase from Streptomyces halstedii. The recombinant enzyme exhibited optimal activity at pH 6.5 and 50 °C and was stable over a broad pH (6–9.5) range and temperature < 50 °C. CelA257 exhibited broad substrate specificity to barley β-glucan, lichenin, CMC, chitosan, laminarin, avicel, and phosphoric acid swollen cellulose (PASC). CelA257 degraded both cellotetrose (G4) and cellppentaose (G5) to cellobiose (G2) and cellotriose (G3). Adding CelA257 increased the release of reducing sugars in crop straw powers, including wheat straw (0.18 mg/mL), rape straw (0.42 mg/mL), rice straw (0.16 mg/mL), peanut straw (0.16 mg/mL), and corn straw (0.61 mg/mL). This study provides a potential additive in biomass saccharification applications.

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“…The ideal resource for the regular generation of value-added chemicals made up of cellulose, hemicellulose, and lignin, biomass has gained a great deal of attention. Each year, around 731 million metric tons of rice straw are generated, with Asia contributing 92% of this amount [3]. Due to the inert qualities of lignin, this agricultural waste cannot be transformed by many typical chemical techniques and is often thrown in the field or burned, generating numerous environmental hazards.…”

Section: Introductionmentioning

confidence: 99%

Probing the Pyrolysis Process of Rice Straw over a “Dual-Catalyst Bed” for the Production of Fuel Gases and Value-Added Chemicals

et al. 2023

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Rice straw is an agricultural byproduct primarily produced in Asian regions. It is crucial to discover an effective method for converting this waste into chemicals that can be utilized to substitute goods derived from fossil fuels. Pyrolysis serves as an interesting procedure to obtain bio-oil from this rice straw. The composition of the bio-oil obtained after the pyrolysis procedure contains a small quantity of value-added chemicals in addition to various gas components in the gas product. Therefore, the development of catalytic systems that improve this pyrolytic reaction is mandatory. Herein, the design of a dual catalyst bed (CEM/ZSM-5) that catalyzes the volatiles that it releases has been developed. The highest output of 42.1 wt.% of bio-oil, 29.9 wt.% of gases and 28.0 wt.% of bio-char was obtained. Nevertheless, the inclusion of single zeolites to biomass yields biofuel outputs of 42.8 wt.%, gas yields of 27.7 wt.%, and a bio-char yielding of 29.5 wt.%. Additionally, the addition of cement to biomass results in a bio-oil yield of 40.4 wt.% and 30.5 wt.% of gas, along with 29.1 wt.% of char. Regarding pyrolysis gas products, the H2 yield in the produced biogas was increased from 35.9 mL/g to 45.7 mL/g, and the CH4 output was increased from 21.1 mL/g to 27.4 mL/g. The bioenergy output was evaluated employing GC-FID and GC-MS (gas and biofuel). The dual catalytic bed had a significant impact on the contents of the generated biofuel, increasing the quantity of hydrocarbons and other value-added compounds.

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Enhanced saccharification of reed and rice straws by the addition of β-1,3-1,4-glucanase with broad substrate specificity and calcium ion

Cited by 9 publications

References 25 publications

Roles of Carbohydrate-Binding Module (CBM) of an Endo-��-1,4-glucanase (Cel5L) from Bacillus sp. KD1014 in Thermostability and Small-Substrate Hydrolyzing Activity

Roles of Carbohydrate-Binding Module (CBM) of an Endo-��-1,4-glucanase (Cel5L) from Bacillus sp. KD1014 in Thermostability and Small-Substrate Hydrolyzing Activity

Biochemical Characterization of Novel GH6 Endoglucanase from Myxococcus sp. B6-1 and Its Effects on Agricultural Straws Saccharification

Probing the Pyrolysis Process of Rice Straw over a “Dual-Catalyst Bed” for the Production of Fuel Gases and Value-Added Chemicals

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