Enhanced salinity tolerance and improved yield properties in Bangladeshi rice Binnatoa through Agrobacterium-mediated transformation of PgNHX1 from Pennisetum glaucum

Islam, Saiful; Tammi, Rumana S; Singla‐Pareek, Sneh L.; Seraj, Zeba I.

doi:10.1007/s11738-009-0443-8

Cited by 14 publications

(10 citation statements)

References 29 publications

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“…Our results on Na + accumulation are in disagreement with the most recent study, , who reported a higher accumulation of Na + in the leaves of transgenic plants, including transgenics harbouring TaNHX1, compared to WT plants under 200 mM salt treatment. However, our results are in line with those reported by Islam et al (2010) who demonstrated that the over-expression of Pennisetum glaucum vacuolar antiporter gene (PgNHX1) in rice led to significantly higher leaf content of K + and less Na + in transgenic plants as compared to wild type plants. Similar results were also reported by Zhao et al (2007) and Wei et al (2010).…”

Section: Discussionsupporting

confidence: 93%

Expression of wheat Na+/H+ antiporter TNHXS1 and H+- pyrophosphatase TVP1 genes in tobacco from a bicistronic transcriptional unit improves salt tolerance

et al. 2012

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Abiotic stress tolerance of plants is a very complex trait and involves multiple physiological and biochemical processes. Thus, the improvement of plant stress tolerance should involve pyramiding of multiple genes. In the present study, we report the construction and application of a bicistronic system, involving the internal ribosome entry site (IRES) sequence from the 5'UTR of the heat-shock protein of tobacco gene NtHSF-1, to the improvement of salt tolerance in transgenic tobacco plants. Two genes from wheat encoding two important vacuolar ion transporters, Na(+)/H(+) antiporter (TNHXS1) and H(+)-pyrophosphatase (TVP1), were linked via IRES to generate the bicistronic construct TNHXS1-IRES-TVP1. Molecular analysis of transgenic tobacco plants revealed the correct integration of the TNHXS1-IRES-TVP1construct into tobacco genome and the production of the full-length bicistronic mRNA from the 35S promoter. Ion transport analyses with tonoplast vesicles isolated from transgenic lines confirmed that single-transgenic lines TVP1cl19 and TNHXS1cl7 had greater H(+)-PPiase and Na(+)/H(+) antiport activity, respectively, than the WT. Interestingly, the co-expression of TVP1 and TNHXS1 increased both Na(+)/H(+) antiport and H(+)-PPiase activities and induced the H(+) pumping activity of the endogenous V-ATPase. Transgenic tobacco plants expressing TNHXS1-IRES-TVP1 showed a better performance than either of the single gene-transformed lines and the wild type plants when subjected to salt treatment. In addition, the TNHXS1-IRES-TVP1 transgenic plants accumulated less Na(+) and more K(+) in their leaf tissue than did the wild type and the single gene-transformed lines. These results demonstrate that IRES system, described herein, can co-ordinate the expression of two important abiotic stress-tolerance genes and that this expression system is a valuable tool for obtaining transgenic plants with improved salt tolerance.

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Section: Discussionsupporting

confidence: 93%

Expression of wheat Na+/H+ antiporter TNHXS1 and H+- pyrophosphatase TVP1 genes in tobacco from a bicistronic transcriptional unit improves salt tolerance

et al. 2012

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“…Our results are consistent with our previous observation of lower Na + in leaves of salt tolerant cowpea transgenics, overexpressing VrNHX1 (Mishra et al, 2014 ). Similar phenomena of lower Na + in leaves transgenic plants overexpressing NHXs genes was also reported in rice (Islam et al, 2010 ), soybean (Li et al, 2010 ). In the transgenic plants examined, the salt-tolerant phenotype was associated with a lower K + content in leaves and roots relative to WT plants.…”

Section: Discussionsupporting

confidence: 81%

Co-expression of Arabidopsis NHX1 and bar Improves the Tolerance to Salinity, Oxidative Stress, and Herbicide in Transgenic Mungbean

Kumar

Kalita

et al. 2017

Front. Plant Sci.

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Mungbean is an important pulse crop extensively cultivated in Southeast Asia for supply of easily digestible protein. Salinity severely limits the growth and productivity of mungbean, and weeding poses nutritional and disease constraints to mungbean cultivation. To pyramid both salt tolerance and protection against herbicide in mungbean, the AtNHX1 encoding tonoplast Na+/H+ antiporter from Arabidopsis, and bar gene associated with herbicide resistance were co-expressed through Agrobacterium-mediated transformation. Stress inducible expression of AtNHX1 significantly improved tolerance under salt stress to ionic, osmotic, and oxidative stresses in transgenic mungbean plants compared to the wild type (WT) plants, whereas constitutive expression of bar provided resistance to herbicide. Compared to WT, transgenic mungbean plants grew better with higher plant height, foliage, dry mass and seed yield under high salt stress (200 mM NaCl) in the greenhouse. The improved performance of transgenic plants under salt stress was associated with enhanced sequestration of Na+ in roots by vacuolar Na+/H+ antiporter and limited transport of toxic Na+ to shoots, possibly by restricting Na+ influx into shoots. Transgenic plants showed better intracellular ion homeostasis, osmoregulation, reduced cell membrane damage, improved photosynthetic capacity, and transpiration rate as compared to WT when subjected to salt stress. Reduction in hydrogen peroxide and oxygen radical production indicated enhanced protection of transgenic plants to both salt- and methyl vialogen (MV)-induced oxidative stress. This study laid a firm foundation for improving mungbean yield in saline lands in Southeast Asia.

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“…However, transgenic switchgrass accumulated significantly higher K + and lower Na + in all tissues compared with control plants (Figure 7), which failed to detect the significant correlation between enhanced salt tolerance and increased accumulation of Na + . The presence of lower Na + in transgenic plants overexpressing NHXs genes was also reported in rice (Islam et al, 2010), soybean (Li et al, 2010), and cowpea (Mishra et al, 2014), These resules challenged the widely accepted notion that NHXs convey salt tolerance by enhancing Na + sequestration into vacuoles, indicating a different regulatory pathways of NHXs in salt tolerance might exist. This speculation was supported by the evidence from recent reverse genetics, in which the critical role of NHXs in K + homeostasis to withstand salt shock was confirmed (Bassil et al, 2011; Barragán et al, 2012; Andrés et al, 2014).…”

Section: Discussionmentioning

confidence: 91%

“…In plants, the sequestration of Na + in vacuole had been identified as an important mechanism to salt tolerance (Apse et al, 1999). However, recent studies have found the opposite result in transgenic rice (Islam et al, 2010), soybean (Li et al, 2010), and cowpea (Mishra et al, 2014), that greater K + , rather than Na + contents were observed under salinity stress. Emerging new reverse genetics evidence has also implicated that NHXs were not indispensable for Na + uptake into vacuoles of Arabidopsis , and confirmed their main role in the regulation of K + homeostasis (Bassil et al, 2011; Barragán et al, 2012; Andrés et al, 2014).…”

Section: Introductionmentioning

confidence: 97%

Enhanced Growth Performance and Salinity Tolerance in Transgenic Switchgrass via Overexpressing Vacuolar Na+ (K+)/H+ Antiporter Gene (PvNHX1)

et al. 2017

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Switchgrass (Panicum virgatum L.) has been increasingly recognized as one of the most valuable perennial bioenergy crop. To improve its biomass production, especially under salt stress, we isolated a putative vacuolar Na+ (K+)/H+ antiporter gene from switchgrass and designated as PvNHX1. Subcellular localization revealed that this protein was localized mainly on the vacuole membrane. The PvNHX1 was found to be expressed throughout the entire growth period of switchgrass, exhibited preferentially expressed in the leaf tissue, and highly induced by salt stress. Transgenic switchgrass overexpressing PvNHX1 showed obvious advantages with respect to plant height and leaf development compared to the wild-type (WT) and transgenic control (EV, expressing the empty vector only) plants, suggesting PvNHX1 may serve as a promoter in switchgrass growth and development. Moreover, transgenic switchgrass were more tolerant than control plants with better growth-related phenotypes (higher shoot height, larger stem diameter, longer leaf length, and width) and physiological capacities (increased proline accumulation, reduced malondialdehyde production, preserved cell membrane integrity, etc.) under high salinity stress. Furthermore, the genes related to cell growth, flowering, and potassium transporters in transgenic switchgrass exhibited a different expression profiles when compared to the control plants, indicating a pivotal function of PvNHX1 in cell expansion and K+ homeostasis. Taken together, PvNHX1 is essential for normal plant growth and development, and play an important role in the response to salt stress by improving K+ accumulation. Our data provide a valuable foundation for further researches on the molecular mechanism and physiological roles of NHXs in plants.

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Enhanced salinity tolerance and improved yield properties in Bangladeshi rice Binnatoa through Agrobacterium-mediated transformation of PgNHX1 from Pennisetum glaucum

Cited by 14 publications

References 29 publications

Expression of wheat Na+/H+ antiporter TNHXS1 and H+- pyrophosphatase TVP1 genes in tobacco from a bicistronic transcriptional unit improves salt tolerance

Expression of wheat Na+/H+ antiporter TNHXS1 and H+- pyrophosphatase TVP1 genes in tobacco from a bicistronic transcriptional unit improves salt tolerance

Co-expression of Arabidopsis NHX1 and bar Improves the Tolerance to Salinity, Oxidative Stress, and Herbicide in Transgenic Mungbean

Enhanced Growth Performance and Salinity Tolerance in Transgenic Switchgrass via Overexpressing Vacuolar Na+ (K+)/H+ Antiporter Gene (PvNHX1)

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