Enhanced salinity tolerance in transgenic mungbean overexpressing Arabidopsis antiporter (NHX1) gene

Sahoo, Debee Prasad; Kumar, Sanjeev; Mishra, Sagarika; Kobayashi, Yuriko; Panda, Sanjib Kumar; Sahoo, Lingaraj

doi:10.1007/s11032-016-0564-x

Cited by 59 publications

(45 citation statements)

References 63 publications

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“…Moreover, strong and stable GUS expression of NHX genes were abundant in most flower organs (stigma, pollen, anther, stamen, petal, etc.) of the transgenic rice (Fukuda et al, 2011), Arabidopsis (Barragán et al, 2012), and mungbean (Sahoo et al, 2016). These results might suggest that NHXs are involved in plant flower development.…”

Section: Discussionmentioning

confidence: 99%

“…However, previous studies in various plant species have demonstrated that overexpressing the single gene, vacuolar Na + (K + )/H + antiporter gene, is a feasible way to create transgenic plants with enhanced salt tolerance (Mishra et al, 2014; Chen et al, 2015; Zhang et al, 2015; Sahoo et al, 2016). Consistent with these studies, transgenic switchgrass overexpressing PvNHX1 exhibited better growth performance (Figure 5) and maintained an improved physiological capacity (Figures 6, Figure S4) comparing to control plants under high salinity stress.…”

Section: Discussionmentioning

confidence: 99%

“…NHXs are ubiquitous to all eukaryotic organisms and implicate a pivotal function in regulating plant responses to salt stress (Zhang et al, 2015; Sahoo et al, 2016), cold tolerance (Li et al, 2007), drought tolerance (Wang et al, 2016) and disease resistance (Chen et al, 2015). The successful expressions of the key enzyme genes related to the NHXs were proved to be valid to enhance the capacity for salt tolerance in diverse species, such as and cowpea (Mishra et al, 2014), tobacco (Chen et al, 2015), alfalfa (Zhang et al, 2015), and mungbean (Sahoo et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…The successful expressions of the key enzyme genes related to the NHXs were proved to be valid to enhance the capacity for salt tolerance in diverse species, such as and cowpea (Mishra et al, 2014), tobacco (Chen et al, 2015), alfalfa (Zhang et al, 2015), and mungbean (Sahoo et al, 2016). In spite of numerous work has focused on use of NHXs in salt tolerance, the mechanism underlying the enhancement of salinity tolerance by NHXs remained unclear (Sahoo et al, 2016; Wang et al, 2016). In plants, the sequestration of Na + in vacuole had been identified as an important mechanism to salt tolerance (Apse et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Growth Performance and Salinity Tolerance in Transgenic Switchgrass via Overexpressing Vacuolar Na+ (K+)/H+ Antiporter Gene (PvNHX1)

et al. 2017

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Switchgrass (Panicum virgatum L.) has been increasingly recognized as one of the most valuable perennial bioenergy crop. To improve its biomass production, especially under salt stress, we isolated a putative vacuolar Na+ (K+)/H+ antiporter gene from switchgrass and designated as PvNHX1. Subcellular localization revealed that this protein was localized mainly on the vacuole membrane. The PvNHX1 was found to be expressed throughout the entire growth period of switchgrass, exhibited preferentially expressed in the leaf tissue, and highly induced by salt stress. Transgenic switchgrass overexpressing PvNHX1 showed obvious advantages with respect to plant height and leaf development compared to the wild-type (WT) and transgenic control (EV, expressing the empty vector only) plants, suggesting PvNHX1 may serve as a promoter in switchgrass growth and development. Moreover, transgenic switchgrass were more tolerant than control plants with better growth-related phenotypes (higher shoot height, larger stem diameter, longer leaf length, and width) and physiological capacities (increased proline accumulation, reduced malondialdehyde production, preserved cell membrane integrity, etc.) under high salinity stress. Furthermore, the genes related to cell growth, flowering, and potassium transporters in transgenic switchgrass exhibited a different expression profiles when compared to the control plants, indicating a pivotal function of PvNHX1 in cell expansion and K+ homeostasis. Taken together, PvNHX1 is essential for normal plant growth and development, and play an important role in the response to salt stress by improving K+ accumulation. Our data provide a valuable foundation for further researches on the molecular mechanism and physiological roles of NHXs in plants.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enhanced Growth Performance and Salinity Tolerance in Transgenic Switchgrass via Overexpressing Vacuolar Na+ (K+)/H+ Antiporter Gene (PvNHX1)

et al. 2017

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“…When the accumulation of salt occurs, plants trigger tissue tolerance by activating compartmentation of salt in vacuoles and tissue protection machinery. and maintained a higher K + /Na + ratio (Zhang and Blumwald, 2001;Apse and Blumwald, 2007;Li et al, 2010;Asif et al, 2011;Fan et al, 2014;Sahoo et al, 2016). The Na + compartmentalization in vacuoles is mediated by a Na + /H + H + -pyrophosphatase gene (AtAVP1) and a vacuolar Na + /H + antiporter gene (AtNHX1) were co-expressed to determine if the compartmentation of Na + in vacuole is feasible to improve salt tolerance in soybean.…”

Section: Researchmentioning

confidence: 99%

Co‐expression of Arabidopsis AtAVP1 and AtNHX1 to Improve Salt Tolerance in Soybean

Nguyen

et al. 2019

Crop Science

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Building a better Mungbean: Breeding for reproductive resilience in a changing climate

Haeften

Dudley

Kang

et al. 2023

Food and Energy Security

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Mungbean (Vigna radiata (L.) R. Wilczek var. radiata) is a significant food and cash crop grown in tropical and subtropical regions. Mungbean production and consumer demand have increased substantially over the last two decades, owing to its agronomic, nutritional and economic benefits. Despite increased breeding efforts and the expansion of mungbean production in various agro‐climatic regions, further production is hindered by low yield and variability, which is partly attributed to the impacts of abiotic stress. Abiotic stress impacts on the physiology, morphology and reproductive ability of mungbean which influences yield. Exposure to abiotic stresses at the reproductive stage is considered the most critical for yield production. In this review, we evaluate how abiotic stress impacts mungbean growth and productivity when occurring during the reproductive stage and traits that may confer adaptation. We present the limitations of current research including limited number of genotypes, lack of field experiments and detailed experimental information. We highlight the opportunities to exploit new tools and technologies, such as high‐throughput phenotyping platforms, gene editing, and genomic selection, to accelerate breeding efforts to develop more resilient mungbean cultivars for today and tomorrow.

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Enhanced salinity tolerance in transgenic mungbean overexpressing Arabidopsis antiporter (NHX1) gene

Cited by 59 publications

References 63 publications

Enhanced Growth Performance and Salinity Tolerance in Transgenic Switchgrass via Overexpressing Vacuolar Na+ (K+)/H+ Antiporter Gene (PvNHX1)

Enhanced Growth Performance and Salinity Tolerance in Transgenic Switchgrass via Overexpressing Vacuolar Na+ (K+)/H+ Antiporter Gene (PvNHX1)

Co‐expression of Arabidopsis AtAVP1 and AtNHX1 to Improve Salt Tolerance in Soybean

Building a better Mungbean: Breeding for reproductive resilience in a changing climate

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