Enhanced <scp>d</scp>‐lactic acid production by recombinant <i>Saccharomyces cerevisiae</i> following optimization of the global metabolic pathway

Yamada, R.; Wakita, Kazuki; Mitsui, Ryosuke; Ogino, Hiroyasu

doi:10.1002/bit.26330

Cited by 49 publications

(27 citation statements)

References 35 publications

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“…Initially, DNA fragments encoding 15 promoters from S. cerevisiae , P. pastoris , and H. polymorpha , were PCR‐amplified using the plasmid library pPPE_LibBTLAnc (Yamada, Kimoto, & Ogino, 2016) as the template, as well as ProF and ProR as primers. All promoters we selected were used for GMES in previous studies (Yamada et al, 2017a; Yamada et al, 2017b; Yamada et al, 2017c; Yamada et al, 2018). Next, DNA fragments encoding eight enzymes ( ERG10 , ERG13 , tHMG1 , ERG12 , ERG8 , ERG19 , IDI1 , and ERG20 ) in the mevalonate pathway and their terminator sequences were PCR‐amplified using S. cerevisiae genomic DNA as the template and corresponding primers.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, the global metabolic engineering strategy (GMES) using the cocktail δ‐integration for yeast has been developed (Yamada, Wakita, & Ogino, 2017a), which involves the simultaneous integration of various multicopy genes via δ‐integration, followed by the selection of desirable transformants in S. cerevisiae . With regard to utilizing the GMES, glycolysis‐related genes (Yamada et al, 2017a), lactate production‐involved genes (Yamada, Wakita, Mitsui, & Ogino, 2017b), 2,3‐butanediol production (Yamada, Wakita, Mitsui, & Ogino, 2017c), and carotenoid production (Yamada, Yamauchi, Ando, Kumata, & Ogino, 2018) in S. cerevisiae were optimized. The GMES is a strategy that comprehensively and randomly modifies the transcription level of genes in target metabolic pathways in yeast.…”

Section: Introductionmentioning

confidence: 99%

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Construction of yeast producing patchoulol by global metabolic engineering strategy

Mitsui

Nishikawa

Yamada

et al. 2020

Biotech & Bioengineering

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Patchoulol is a sesquiterpene alcohol found in the leaves of the patchouli plant that can be extracted by steam distillation. Notably, patchoulol is an essential natural product frequently used in the chemical industry. However, patchouli produces an insignificant amount of patchoulol, not to mention steam distillation, and requires a lot of energy and time. Recombinant microorganisms that can be cultured in mild conditions and can produce patchoulol from renewable biomass resources may be a promising alternative. We previously developed the global metabolic engineering strategy (GMES), which produces a comprehensive metabolic modification in yeast, using the cocktail δ‐integration method. In this study, we aimed to produce patchoulol by modifying engineered yeast. The expression of nine genes involved in patchoulol synthesis was modulated using GMES. Regarding patchoulol production, the resultant strain, YPH499/PAT167/MVA442, showed a concentration of 42.1 mg/L, a production rate of 8.42 mg/L/d, and a yield of 2.05 mg/g‐glucose, respectably. These concentration values, production rate, and yield obtained through batch‐fermentation in this study were high level when compared to previously reported recombinant microorganism studies. GMES could be used as a potential strategy for producing secondary metabolites from plants in recombinant Saccharomyces cerevisiae.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Construction of yeast producing patchoulol by global metabolic engineering strategy

Mitsui

Nishikawa

Yamada

et al. 2020

Biotech & Bioengineering

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“…This condition not only reduces the input of neutralization agents, but also allows the utilization of a wider range of substrates [41]. For example, the combination of d-LDH genes from Leuconostoc mesenteroides with Saccharomyces cerevisiae resulted in titers as high as 112 g·L −1 of d-(−)-LA in a fed-batch process at a productivity of 2.2 g·L −1 ·h −1 [42][43][44].…”

Section: Lactic Acidmentioning

confidence: 99%

Multi-Product Lactic Acid Bacteria Fermentations: A Review

Mora-Villalobos¹,

Montero-Zamora²,

Barboza

et al. 2020

Fermentation

128

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Industrial biotechnology is a continuously expanding field focused on the application of microorganisms to produce chemicals using renewable sources as substrates. Currently, an increasing interest in new versatile processes, able to utilize a variety of substrates to obtain diverse products, can be observed. A robust microbial strain is critical in the creation of such processes. Lactic acid bacteria (LAB) are used to produce a wide variety of chemicals with high commercial interest. Lactic acid (LA) is the most predominant industrial product obtained from LAB fermentations, and its production is forecasted to rise as the result of the increasing demand of polylactic acid. Hence, the creation of new ways to revalorize LA production processes is of high interest and could further enhance its economic value. Therefore, this review explores some co-products of LA fermentations, derived from LAB, with special focus on bacteriocins, lipoteichoic acid, and probiotics. Finally, a multi-product process involving LA and the other compounds of interest is proposed.Fermentation 2020, 6, 23 2 of 21 by-products, were proposed to improve industrial single-product biotechnological processes [2]. One example is the use of cellulose and hemicellulose from sugarcane bagasse (a residue obtained during the bioethanol production and usually used for energy generation) for the production of value-added chemicals [3]. Likewise, glycerol, a by-product of the biodiesel industry with low value in the market, is targeted as a molecule of interest in fermentation processes. Such multi-product processes, based on the conversion of renewable materials into biobased products, are known as biorefineries [4].The study, development, and application of robust microbial strains, able to utilize a variety of substrates and to produce a wide range of products, is considered a milestone in the development of biorefineries [4]. Lactic acid bacteria (LAB) are a diverse group with recognized potential for the development of integrated biorefineries [5]. LAB are non-sporulating, non-motile, acid-tolerant, non-respiring but aerotolerant, catalase-negative, Gram-positive cocci or rods. They are characterized by the production of lactic acid (LA) as the major end metabolic product of carbohydrate fermentation [6][7][8][9]. Given the lack of a functional respiratory system, LAB obtain energy through substrate-level phosphorylation following two metabolic pathways for hexose fermentation, i.e., homofermentative and heterofermentative. As shown in Figure 1, the first pathway is based on glycolysis with the production of mainly LA, whereas the second one, known as the pentose phosphate pathway, is characterized for the production of CO 2 and ethanol, or acetate in addition to LA [6].

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“…The Escherichia coli strain HST08 (TaKaRa Bio Inc., Otsu, Japan) was used as the host for recombinant DNA manipulations. Recombinant E. coli cells were cultivated in LB medium supplemented with 100 µg mL −1 ampicillin sodium salt.…”

Section: Methodsmentioning

confidence: 99%

“…S. cerevisiae strain YPH499 (NBRC 10505) was used as the host for secretory expression of BTL2 from B. thermocatenulatus . Recombinant S. cerevisiae cells were cultivated in YPD medium, SD medium, SDC medium (SD containing 20 g L −1 casamino acid and 50 mM sodium phosphate [pH 6.5]) or SD + tributyrin medium (SD containing 0.5% [v/v] tributyrin and 50 mM sodium phosphate [pH 6.5]) supplemented with the appropriate amino acids and nucleic acids. Each agar plate contained 20 g L −1 of agar.…”

Section: Methodsmentioning

confidence: 99%

Secretory Overexpression of Bacillus thermocatenulatus Lipase in Saccharomyces cerevisiae Using Combinatorial Library Strategy

Kajiwara

Yamada

Ogino

2018

Biotechnology Journal

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Simple and cost-effective lipase expression host microorganisms are highly desirable. A combinatorial library strategy is used to improve the secretory expression of lipase from Bacillus thermocatenulatus (BTL2) in the culture supernatant of Saccharomyces cerevisiae. A plasmid library including expression cassettes composed of sequences encoding one of each 15 promoters, 15 secretion signals, and 15 terminators derived from yeast species, S. cerevisiae, Pichia pastoris, and Hansenula polymorpha, is constructed. The S. cerevisiae transformant YPH499/D4, comprising H. polymorpha GAP promoter, S. cerevisiae SAG1 secretion signal, and P. pastoris AOX1 terminator, is selected by high-throughput screening. This transformant expresses BTL2 extra-cellularly with a 130-fold higher than the control strain, comprising S. cerevisiae PGK1 promoter, S. cerevisiae α-factor secretion signal, and S. cerevisiae PGK1 terminator, after cultivation for 72 h. This combinatorial library strategy holds promising potential for application in the optimization of the secretory expression of proteins in yeast.

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Enhanced d‐lactic acid production by recombinant Saccharomyces cerevisiae following optimization of the global metabolic pathway

Cited by 49 publications

References 35 publications

Construction of yeast producing patchoulol by global metabolic engineering strategy

Construction of yeast producing patchoulol by global metabolic engineering strategy

Multi-Product Lactic Acid Bacteria Fermentations: A Review

Secretory Overexpression of Bacillus thermocatenulatus Lipase in Saccharomyces cerevisiae Using Combinatorial Library Strategy

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