Enhanced secretion of oxytocin from bovine granulosa cells treated with adrenal steroids

Lück, Martin

doi:10.1530/jrf.0.0830901

Cited by 20 publications

(9 citation statements)

References 30 publications

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“…Because glucocorticoid is only synthesized in the adrenal cortex and is delivered systemically to the whole body, the presence of 11 -HSD is crucial for glucocorticoid target organs such as the liver and lung for modulating local concentrations of active glucocorticoid (Krozowski et al 1999). The expression of 11HSD1 and 11HSD2 in bovine ovarian tissues, together with the reported direct glucocorticoid action in cultured follicular cells (Luck 1988, Kawate et al 1993, Spicer & Chamberlain 1998, indicates that the bovine ovary is a glucocorticoid target organ which is also capable of modulating the local glucocorticoid environment.…”

Section: Discussionmentioning

confidence: 98%

“…The ovary expresses glucocorticoid receptor (GR) and direct action of glucocorticoid on various ovarian functions has been reported in several species (Hsueh & Erickson 1978, Luck 1988, Fitzpatrick & Richards 1991, Kawate et al 1993, Urban et al 1994, Spicer & Chamberlain 1998, Gaytan et al 2002, Yang et al 2002. The ovary does not produce glucocorticoid per se and the access of active glucocorticoid (i.e.…”

Section: Introductionmentioning

confidence: 99%

“…It has been shown to enhance progesterone (P 4 ) (Kawate et al 1993) and oxytocin production (Luck 1988) in cultured granulosa cells, and insulin-like growth factor-stimulated P 4 and androstenedione production in cultured thecal cells (Spicer & Chamberlain 1998), whereas it was shown to inhibit production of oestradiol-17 (E 2 ) and expression of luteinising hormone (LH)-receptor in cultured granulosa cells (Kawate et al 1993). For healthy follicular development, therefore, it is necessary that the expression of these factors is properly regulated in the face of circulating glucocorticoid.…”

Section: Introductionmentioning

confidence: 99%

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Expression of 11beta-hydroxysteroid dehydrogenases in bovine follicle and corpus luteum

Tetsuka¹,

Yamamoto²,

Hayashida³

et al. 2003

Journal of Endocrinology

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In glucocorticoid target organs, local concentrations of active glucocorticoid are determined by the relative expression of two 11 -hydroxysteroid dehydrogenases (HSDs): bi-directional 11 -HSD type1 (11HSD1) that mainly activates cortisone to cortisol, and dehydrogenase 11 -HSD type2 (11HSD2) that inactivates cortisol to cortisone. In this study, we examined the expression of mRNA encoding these two 11 -HSDs in bovine granulosa cells harvested from preovulatory follicles and corpora lutea (CL). Ovaries were obtained from Holstein cows at a local slaughterhouse. Follicles larger than 10 mm in diameter and CL were dissected and follicular fluid and granulosa cells were taken. Corpora lutea were weighed and their stages were morphologically assessed (stage I, days 1-4; stage II, days 5-10; stage III, days 11-17; stage IV, days 8-20). Follicles were classified into four groups according to their hormonal status (oestradiol (E 2 ): progesterone (P 4 )>1: oestrogen active; E 2 :P 4 <1: oestrogen inactive) and stage of the oestrous cycle (luteal or follicular phase). Total RNA was extracted with phenol-chloroform and subjected to a semi-quantitative RT-PCR for 11HSD1, 11HSD2 and -actin. Concentrations of steroids in follicular fluid were determined by an enzyme immunoassay. In granulosa cells, only 11HSD1 mRNA was detected. There was a negative correlation between the expression of 11HSD1 and the concentration of cortisol in follicular fluid (P<0·05), indicating 11HSD1 may act as a dehydrogenase in the bovine follicle. Both types of 11 -HSDs were expressed in CL. The levels of mRNA for both isozymes were high in stage I and II, and were decreased in stage III CL. In stage IV CL, the expression of 11HSD2 but not 11HSD1 mRNA increased. These results indicate that the bovine granulosa cells and CL express 11HSD1 and 11HSD2, and they may play an important physiological role in the bovine ovary through modulating the local glucocorticoid environment.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Expression of 11beta-hydroxysteroid dehydrogenases in bovine follicle and corpus luteum

Tetsuka¹,

Yamamoto²,

Hayashida³

et al. 2003

Journal of Endocrinology

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“…It has been well documented that ovulation is an inflammatory-like reaction that involves many inflammatory mediators such as leucocytes, cytokines, prostaglandins, steroids and proteases (Espey 1980, 1994, Richards et al 2002. Glucocorticoid has been shown to regulate these factors in the ovary of rats and cattle (Adashi et al 1981, Schoonmaker & Erickson 1983, Luck 1988, Wang & Leung 1989, Jia et al 1990, Kol et al 1998, Irahara et al 1999. Glucocorticoid has also been suggested to protect ovulating follicles by suppressing apoptosis in granulosa cells and inducing apoptosis in infiltrating leucocytes (Amsterdam & Sasson 2002, Sasson & Amsterdam 2003.…”

Section: Glucocorticoid Metabolism In Bovine Cocmentioning

confidence: 99%

Glucocorticoid metabolism in the bovine cumulus–oocyte complex matured in vitro

et al. 2016

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Glucocorticoid action in target organs is regulated by relative activities of 11b-HSD type 1 (HSD11B1) that mainly converts cortisone to active cortisol and type 2 (HSD11B2) that inactivates cortisol to cortisone. HSD11Bs have been shown to be expressed in the ovary of various species. However, little is known about the expression and activity of HSD11Bs in the bovine cumulus-oocyte complex (COC). In the present study, we investigated the expression and activities of HSD11Bs in in vitro-matured (IVM) bovine COCs. Bovine COCs were matured in M199 supplemented with or without FSH and FCS. The expression of HSD11B1 and HSD11B2 was measured by using quantitative RT-PCR in denuded oocytes (DO) and cumulus cells (CC). Reductive and oxidative activities of HSD11Bs were determined by radiometric conversion assay using labeled cortisol, cortisone or dexamethasone in intact COCs, DO or CC in the presence or absence of 11-keto-progesterone (11kP), a selective inhibitor of HSD11B2. The presence of HSD11Bs in the oocyte was examined by immunofluorescence microscopy. Oocytes exclusively expressed HSD11B2 and its expression and activity were largely unchanged during IVM. CC, on the other hand, exclusively expressed HSD11B1 and its expression and activity were upregulated as IVM progressed. As a result, the net glucocorticoid metabolism shifted from inactivation to activation towards the end of IVM. These results indicate that the bovine COC is capable of modulating local glucocorticoid concentration and, by doing so, may create an environment that is favorable to ovulating oocyte for maturation, fertilization and subsequent development.

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“…As an autocrine factor, OT supports the growth and maturation of the ovarian follicles (Voss andFortune 1993, Jo andFortune 2003). In addition, Besides the prostaglandins and oestrogens in luteal cells and LH, and FSH in granulosa cells (Wathes 1989, Kotwica et al 1990, cortisol was also found to increase OT secretion from bovine ovarian cells (Luck 1988. Glucocorticosteroids (GCs) are steroid hormones produced in the zona fasciculata and the reticulate of the adrenal cortex.…”

Section: Introductionmentioning

confidence: 99%

Effect of cortisol on neurophysin I/oxytocin and peptidyl glycine-α-amidating mono-oxygenase mRNA expression in bovine luteal and granulosa cells

Ziółkowska¹,

Mlynarczuk²,

Kotwica³

2013

Polish Journal of Veterinary Sciences

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Cortisol stimulates the synthesis and secretion of oxytocin (OT) from bovine granulosa and luteal cells, but the molecular mechanisms of cortisol action remain unknown. In this study, granulosa cells or luteal cells from days 1-5 and 11-15 of the oestrous cycle were incubated for 4 or 8 h with cortisol (1x10 -5 , 1x10 -7 M). After testing cell viability and hormone secretion (OT, progesterone, estradiol), we studied the effect of cortisol on mRNA expression for precursor of OT (NP-I/OT) and peptidyl glycine-α-amidating mono-oxygenase (PGA). The influence of RU 486 (1x10 -5 M), a progesterone receptor blocker and inhibitor of the glucocorticosteroid receptor (GR), on the expression for both genes was tested. Cortisol increased the mRNA expression for NP-I/OT and PGA in granulosa cells and stimulated the expression for NP-I/OT mRNA in luteal cells obtained from days 1-5 and days 11-15 of the oestrous cycle. Expression for PGA mRNA was increased only in luteal cells from days 11-15 of the oestrous cycle. In addition, RU 486 blocked the cortisol-stimulated mRNA expression for NP-I/OT and PGA in both types of cells. These data suggest that cortisol affects OT synthesis and secretion in bovine ovarian cells, by acting on the expression of key genes, that may impair ovary function.

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Enhanced secretion of oxytocin from bovine granulosa cells treated with adrenal steroids

Cited by 20 publications

References 30 publications

Expression of 11beta-hydroxysteroid dehydrogenases in bovine follicle and corpus luteum

Expression of 11beta-hydroxysteroid dehydrogenases in bovine follicle and corpus luteum

Glucocorticoid metabolism in the bovine cumulus–oocyte complex matured in vitro

Effect of cortisol on neurophysin I/oxytocin and peptidyl glycine-α-amidating mono-oxygenase mRNA expression in bovine luteal and granulosa cells

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