Enhanced silver nanoparticle synthesis by optimization of nitrate reductase activity

Vaidyanathan, Ramanathan; Gopalram, Shubaash; Kalishwaralal, Kalimuthu; Deepak, Venkataraman; Pandian, Sureshbabu Ram Kumar; Gurunathan, Sangiliyandi

doi:10.1016/j.colsurfb.2009.09.006

Cited by 173 publications

(87 citation statements)

References 30 publications

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“…During the reduction, nitrate is converted into nitrite and the electron is transferred to the silver ion; hence, the silver ion is reduced to silver (Ag + to Ag 0 ). This has been said to be observed in Bacillus licheniformis which is known to secrete NADPH and NADPH-dependent enzymes like nitrate reductase that effectively converts Ag + to Ag 0 [27]. The mechanism was further confirmed by using purified nitrate reductase from Fusarium oxysporum and silver nitrate along with NADPH in a test tube, and the change in the color of the reaction mixture to brown and further analysis confirmed that silver nanoparticles were obtained [28].…”

Section: Silver-synthesizing Bacteriamentioning

confidence: 70%

Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects

Sukumaran¹,

Poulose²

2012

Int Nano Lett

1,956

1,099

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Silver nanoparticles are nanoparticles of silver which are in the range of 1 and 100 nm in size. Silver nanoparticles have unique properties which help in molecular diagnostics, in therapies, as well as in devices that are used in several medical procedures. The major methods used for silver nanoparticle synthesis are the physical and chemical methods. The problem with the chemical and physical methods is that the synthesis is expensive and can also have toxic substances absorbed onto them. To overcome this, the biological method provides a feasible alternative. The major biological systems involved in this are bacteria, fungi, and plant extracts. The major applications of silver nanoparticles in the medical field include diagnostic applications and therapeutic applications. In most of the therapeutic applications, it is the antimicrobial property that is being majorly explored, though the anti-inflammatory property has its fair share of applications. Though silver nanoparticles are rampantly used in many medical procedures and devices as well as in various biological fields, they have their drawbacks due to nanotoxicity. This review provides a comprehensive view on the mechanism of action, production, applications in the medical field, and the health and environmental concerns that are allegedly caused due to these nanoparticles. The focus is on effective and efficient synthesis of silver nanoparticles while exploring their various prospective applications besides trying to understand the current scenario in the debates on the toxicity concerns these nanoparticles pose.

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Section: Silver-synthesizing Bacteriamentioning

confidence: 70%

Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects

Sukumaran¹,

Poulose²

2012

Int Nano Lett

1,956

1,099

View full text Add to dashboard Cite

show abstract

“…The most widely accepted mechanism of silver biosynthesis is the presence of the nitrate reductase enzyme. The enzyme converts nitrate into nitrite and the electron is transferred to the silver ion; hence, the silver ion is reduced to silver (Ag + to Ag 0 ) (Vaidyanathan et al, 2010). It is worth noting that the pH of the culture supernatant was adjusted to pH 8.5 with NaOH.…”

Section: Resultsmentioning

confidence: 99%

Silver nanoparticles as an antimicrobial agent: A case study on <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> as models for Gram-positive and Gram-negative bacteria

Gomaa

2017

J. Gen. Appl. Microbiol.

130

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“…When the colloidal particles are much smaller than the wavelength of visible light, the solutions have a yellow colour with an intense band in the 380-400 nm range and other less intense or smaller bands at a longer wavelength in the absorption spectrum. This band is attributed to collective excitation of the electron gas in the particles, with a periodic change in electron density at the surface (surface plasmon absorption) [5].…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus Mediated Synthesis of Silver Oxide Nanoparticles

Dhoondia

Chakraborty

2012

Nanomaterials and Nanotechnology

138

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The ability of prokaryotic microorganisms to reduce the inorganic metals has opened up an exciting eco-friendly approach towards the development of natural 'nano-factories'. However, a number of issues have to be addressed from the nanotechnological and microbiological point of view before such a biosynthesis approach can compete with the existing physical and chemical methods. This report investigates the synthesis of silver oxide nanoparticles using Lactobacillus mindensis, isolated using fixer solution from an X-ray photographic laboratory. Nanoparticles obtained were characterized by means of UV-vis spectroscopy, transmission electron microscopy (TEM), and X-ray diffraction (XRD). The UV-vis spectrum shows the absorbance maximum at 430 nm, which is a characteristic of surface plasmon resonance of silver. Further, the presence of stable nanoparticles in the range of 2-20 nm was determined using TEM analysis. Silver nanoparticles in the form of silver oxide were confirmed in the XRD study. In conclusion, Lactobacillus mindensis serves as a promising candidate in the quest to synthesize silver oxide nanoparticles through green chemistry.

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Enhanced silver nanoparticle synthesis by optimization of nitrate reductase activity

Cited by 173 publications

References 30 publications

Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects

Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects

Silver nanoparticles as an antimicrobial agent: A case study on <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> as models for Gram-positive and Gram-negative bacteria

Lactobacillus Mediated Synthesis of Silver Oxide Nanoparticles

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