Enhanced Skin Regeneration by Herbal Extract‐Coated Poly‐L‐Lactic Acid Nanofibrous Scaffold

Abstract

The wound healing process is directly related to the type of treatment. Existing methods of treatment are not responsive enough for severe wounds. The aim of this study was the potential capacity investigation of poly-L-lactic acid (PLLA) nanofibrous scaffolds coated by aloe vera gel for wound dressing applications. In this study, electrospinning method was used for preparing PLLA nanofibers, and after characterization by SEM and MTT, its influence on the wound healing process was investigated with and without aloe vera gel as a wound dressing in full-thickness skin defect in mice. Band-Aids were used as a positive control and vaseline gauze as a negative control. SEM and MTT assays confirmed the nanometer size and biocompatibility of fabricated nanofibers. Macroscopic and histopathological characteristics were evaluated at the end of days 7, 12, and 17 and their results showed that the gel-coated scaffold accelerated the wound-healing process compared with other groups. At the end of the experiment, it was shown that during the whole time of study, gel-coated scaffold had the highest overall repair score. Therefore, gel-coated PLLA scaffold would be an ideal construct for wound healing and skin regenerative medicine application.

<p>Novel Drug Delivery Systems for Loading of Natural Plant Extracts and Their Biomedical Applications</p>

“…201,202 More examples of herbal loaded nanofibers are displayed in Table 1. [203][204][205][206][207] Polymersome (PS) Polymersome is a self-assembled polymeric nanosphere vesicle that may have relatively thick membranes (up to 40 nm), which are formed by synthetic amphiphilic block copolymers ( Figure 14). 208 They are able of incorporating hydrophilic and nonhydrophilic drugs, proteins, peptides, DNA and RNA fragments in their membrane which acts as a barrier to protect them from the biological environment.…”

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Synthetic Polymers

“…PLA nanofibers have also been widely used for wound dressing applications, where they were loaded with various bioactive molecules improving wound healing and preventing microbial infection. Examples include PLLA/zein nanofibrous mats loaded with Rana chensinensis skin peptides with antibacterial and antioxidative activity [76], electrospun PLLA nanofibrous membranes coated by an Aloe vera gel [77], nanofibrillar matrices prepared from blends of PCL and PDLLA loaded with ciprofloxacin [78] or composite electrospun membranes containing polylactide:poly(vinyl pyrrolidone)/polylactide:poly(ethylene glycol) (PLA:PVP/ PLA:PEG) core/shell fibers, designed for treatment of burns and loaded with curcumin and HHC36 antimicrobial peptides [8].…”

Recent Achievements in Bone and Skin Tissue Engineering in Iran

“…These polymers have been used with synthetic polymers such as PU, DPCL, PEGF, PLA, PDMS, and so forth. Also, these wound dressings have been fabricated in different forms including films , 3D porous scaffolds and mainly nanofibrous form using electrospinning .…”