Enhanced SNP-sensing using DNA-templated reactions through confined hybridization of minimal substrates (CHOMS)

Abstract

Confined hybridization of a minimal substrate: striking the right balance between specificity and turnover

A minimal hybridization chain reaction (HCR) system using peptide nucleic acids

“…21 The faster reaction for the second may be explained by additional base stacking interaction between pegIF and DpegH1, mediated by the formation of a pegIF:pegH2 duplex. [35][36][37] These results are consistent with previous PAGE results, which showed that some of the initiator remained unreacted when using a stoichiometric ratio of reagents (Fig. 2B, lane H1 + H2 + 1 eq.…”

Photocatalysis in Chemical Biology: Extending the Scope of Optochemical Control and Towards New Frontiers in Semisynthetic Bioconjugates and Biocatalysis

“…The photo‐excited catalyst is reduced (SET with sodium ascorbate or NADPH) followed by a SET with the pyridinium moiety ( Figure 4 ,A ). This reaction was extended to immolative linkers to cage phenols and amines in vitro and in cellulo ( Figure 4 ,B ) [42,44,48–51] . Notably, the reduction of pyridinium‐based immolative linkers proved to be much faster than an azide‐based linker [48] .…”

“…This reaction was extended to immolative linkers to cage phenols and amines in vitro and in cellulo (Figure 4,B). [42,44,[48][49][50][51] Notably, the reduction of pyridinium-based immolative linkers proved to be much faster than an azide-based linker. [48] In further developments, it was shown that bioluminescence could be used for the photoexcitation of the ruthenium catalyst, leading to the development of semi-synthetic protein sensor that responds to an analyte with a photocatalyzed drug uncaging (Figure 4,D).…”

Visible Light Photochemical Reactions for Nucleic Acid-Based Technologies

“…Inspired by this remarkable achievement, a pair of Ru-photocatalysts and pyridinium linkers has been further utilized to design various nucleic acid-sensing techniques for the detection of miRNA [ 82 , 83 ], double-stranded RNA [ 84 ], and single nucleotide polymorphism (SNP) [ 85 ].…”