Enhanced stability of the cloned Bacillus subtilis alkaline protease gene in alginate-immobilized B. subtilis cells

Zaghloul, Taha I.; Hendawy, H.M.; Assar, Samy El; Mostafa, M. A.

doi:10.1016/s0141-0229(02)00046-7

Cited by 7 publications

(4 citation statements)

References 22 publications

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“…Similar observations were reported by Jamuna and Ramakrishna (1992). Zaghloul et al (2002) suggested that B. subtilis cells appeared to be more active than alginate immobilized cells. This might be due to the fact that viable cells of the free culture were little higher than that of the immobilized culture.…”

Section: Production Of Alkaline Protease In Batch Culturesupporting

confidence: 87%

Production of Bacillus licheniformis ATCC 21415 alkaline protease in batch, repeated batch and continuous culture

Samia¹,

Ahmed²

2010

MJM

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Bacillus licheniformis ATCC 21415 cells were immobilized on different carriers using different methods of immobilization including physical adsorption, covalent binding, ionic binding and entrapment. The immobilized cells were prepared by covalent binding on wool (as a new carrier) through 1% glutaraldehyde had the highest enzyme activity (9.0 U/mL) with the highest specific productivity (6.17 U/g wet cells/h). Alkaline protease production and the stability of biocatalyst were investigated in both free and immobilized cells. The results showed that the immobilized cells were more efficient for enzyme production by repeated batch fermentation (5 cycles, 480 h) with 57% residual activity whereas the free cells retained 35% after 2 cycles. In continuous production the highest enzyme activity (9.9 U/mL) was obtained at a dilution rate of 0.1/h while the highest enzyme yield (763.6 U/h) and the highest reactor productivity (3.32 U/mL/h) were attained at a dilution rate of 0.4/h. Packed-bed bioreactor was a successful method for continuous production of alkaline protease for a long time (168 h) with 53% relative activity. The bioreactor affected the highest specific productivity (118.2 U/g wet cells/h) which was 12-24 times higher than other systems of enzyme production.

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Section: Production Of Alkaline Protease In Batch Culturesupporting

confidence: 87%

Production of Bacillus licheniformis ATCC 21415 alkaline protease in batch, repeated batch and continuous culture

Samia¹,

Ahmed²

2010

MJM

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“…These observations have been confirmed by several authors who investigated the influence of oxygen supply on plasmid stability, and in all studies stability was highest at 100% oxygen saturation [60,107]. Zaghlou et al [108] investigated the stability of a multicopy plasmid that carried the aprE gene encoding alkaline protease. As results showed, plasmid stability reached 83% for Bacillus subtilis cells immobilized in alginate [108].…”

Section: Metabolic Responses To Immobilizationmentioning

confidence: 66%

“…Zaghlou et al [108] investigated the stability of a multicopy plasmid that carried the aprE gene encoding alkaline protease. As results showed, plasmid stability reached 83% for Bacillus subtilis cells immobilized in alginate [108]. Recent reports on the higher retention of plasmid-bearing cells have further extended the scope of whole-cell immobilization to recombinant product formation.…”

Section: Metabolic Responses To Immobilizationmentioning

confidence: 99%

Metabolic Responses of Bacterial Cells to Immobilization

2016

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In recent years immobilized cells have commonly been used for various biotechnological applications, e.g., antibiotic production, soil bioremediation, biodegradation and biotransformation of xenobiotics in wastewater treatment plants. Although the literature data on the physiological changes and behaviour of cells in the immobilized state remain fragmentary, it is well documented that in natural settings microorganisms are mainly found in association with surfaces, which results in biofilm formation. Biofilms are characterized by genetic and physiological heterogeneity and the occurrence of altered microenvironments within the matrix. Microbial cells in communities display a variety of metabolic differences as compared to their free-living counterparts. Immobilization of bacteria can occur either as a natural phenomenon or as an artificial process. The majority of changes observed in immobilized cells result from protection provided by the supports. Knowledge about the main physiological responses occurring in immobilized cells may contribute to improving the efficiency of immobilization techniques. This paper reviews the main metabolic changes exhibited by immobilized bacterial cells, including growth rate, biodegradation capabilities, biocatalytic efficiency and plasmid stability.

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“…Our results were in close agreement to the Ahmad and Abdel-Fattah [14] findings, who also reported lower activity of the immobilized cells when they immobilized bacterial cells on different carriers by different method. Bacillus subtilis free cells were more efficient than immobilized cells for alkaline protease production, reported by Zaghloul et al [40]. Less accessibility of nutrients to immobilized cells and low availability of oxygen can cause decreased activity of immobilized cells.…”

Section: Immobilization Of Bacillus Subtilis Bsu-5 Mutantmentioning

confidence: 89%

Hyper-production of Alkaline Protease by Mutagenic Treatment of Bacillus subtilis M-9 using Agroindustrial Wastes in Submerged Fermentation

Bukhari¹

2013

J Microb Biochem Technol

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Green chemistry technologies are the powerful tool for the management of environmental wastes challenges. Agro industrial residues are composed of complex polysaccharides that support the microbial growth for the production of useful products (enzymes, organic acids, drugs, etc.). Disposal and environment friendly management of these wastes has become a global priority. The aim of present investigation was to improve the alkaline protease yield by treating the parent Bacillus subtilis M-9 strain with different mutagens UV-irradiations, N-methyl-N-nitro-N-nitrosoguinidine (NTG), Ethidium bromide (EB), using agroindustrial wastes (banana stalk and corn stover) in submerged fermentation. Fifteen positive mutants were selected on skim milk agar plates for shake flask experiments. BSU-5 mutant strain showed 81.21± 3.24 PU/mL alkaline protease activity higher than parent strain (23.57 ± 1.19 PU/mL) in optimized fermentation medium. The fermentation profile like pH (9), temperature (45°C), inoculum size (2 mL), incubation time (24 hrs, and kinetic parameters such as u (h -1

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Enhanced stability of the cloned Bacillus subtilis alkaline protease gene in alginate-immobilized B. subtilis cells

Cited by 7 publications

References 22 publications

Production of Bacillus licheniformis ATCC 21415 alkaline protease in batch, repeated batch and continuous culture

Production of Bacillus licheniformis ATCC 21415 alkaline protease in batch, repeated batch and continuous culture

Metabolic Responses of Bacterial Cells to Immobilization

Hyper-production of Alkaline Protease by Mutagenic Treatment of Bacillus subtilis M-9 using Agroindustrial Wastes in Submerged Fermentation

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