Enhanced sulfamethoxazole degradation through ammonia oxidizing bacteria co-metabolism and fate of transformation products

Kassotaki, Elissavet; Buttiglieri, Gianluigi; Ferrando-Climent, Laura; Rodríguez-Roda, Ignasi; Pijuan, Maite

doi:10.1016/j.watres.2016.02.022

Cited by 232 publications

(97 citation statements)

References 47 publications

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“…related OTUs, which were also encountered in aromatic hydrocarbon utilizing or oil degrading cultures . The other alternative would be co‐metabolism of SMX with ammonia oxidation as commonly observed for various pollutants . Co‐metabolism and degradation of SMX was similarly reported by Kassotaki et al .…”

Section: Resultssupporting

confidence: 91%

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Chronic impact of sulfamethoxazole: how does process kinetics relate to metabolic activity and composition of enriched nitrifying microbial culture?

Katipoglu‐Yazan

Ubay‐Cokgor

Orhon

2018

J of Chemical Tech & Biotech

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BACKGROUND This study investigated utilization kinetics of ammonia‐nitrogen induced by chronic sulfamethoxazole (SMX) exposure on biomass with an enriched nitrifying community. A lab‐scale activated sludge system was supplied with 100 mg COD L‐1 of peptone mixture and 50 mg N L‐1 of ammonia nitrogen at a SRT of 15 days. At steady‐state, the reactor was operated with additional daily SMX dosing of 50 mg L‐1 for 35 days. Profiles of oxygen uptake rates and nitrogen fractions obtained in respirometric/batch experiments were used for estimation of nitrification kinetics. RESULTS Acute inhibitory impact of SMX was expressed with high levels of half saturation constants and endogenous decay rates. After 35 days, half saturation constants significantly increased while higher active biomass fractions and recovered endogenous decay rates were estimated. At the end of the acclimation phase, 92% nitrification was achieved with 10 mg L‐1 SMX utilization. CONCLUSION Interpretation of modeling results with the outcomes of molecular analysis facilitated explanation of autotrophic/heterotrophic bacteria behavior in the course of SMX exposure. Removal of SMX may be attributed to co‐metabolism with ammonia oxidation and/or activity of SMX utilizing heterotrophic bacteria. © 2017 Society of Chemical Industry

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Section: Resultssupporting

confidence: 91%

“…Co‐metabolism and degradation of SMX was similarly reported by Kassotaki et al . supporting our findings . In their work, SMX transformation was observed and a correlation was found between ammonia oxidation rates and SMX removal by enriched AOB culture in both absence and presence of acetate.…”

Section: Resultsmentioning

confidence: 99%

Chronic impact of sulfamethoxazole: how does process kinetics relate to metabolic activity and composition of enriched nitrifying microbial culture?

Katipoglu‐Yazan

Ubay‐Cokgor

Orhon

2018

J of Chemical Tech & Biotech

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“…These results are in agreement with the hypothesis reported in literature of significant differences in microbial community structure and metabolic function between different CW designs (Barbieri et al, 2012;Button et al, 2015;Kassotaki et al, 2016;Pelissari et al, 2017a) and may justify the differences in removal capacity observed for conventional water quality parameters and PPCPs in the investigated wetlands.…”

Section: Fluorescent Organic Matter Removal In Constructed Wetlandssupporting

confidence: 93%

“…The occurrence of a larger redox range under conditions of partial saturation may enable complex microbial interactions under aerobic and anaerobic microenvironments or promote the presence of certain microbial communities that can influence the removal of some contaminants. For example, the removal of sulfamethoxazole has been shown to be influenced by the occurring nitrification and denitrification conditions, which influence the microbial population in the bed reactor [39,40]. In addition, the microbial community metabolic function has been demonstrated to be significantly different between different CW designs when treating domestic wastewater [41].…”

Section: Behavior Of Pharmaceuticals and Personal Care Productsmentioning

confidence: 99%

Removal of organic carbon, nitrogen, emerging contaminants and fluorescing organic matter in different constructed wetland configurations

Sgroi

Pelissari

Roccaro

et al. 2018

Chemical Engineering Journal

126

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The elimination of organic carbon, nitrogen, five emerging organic contaminants (EOCs) and fluorescence signature was evaluated in two treatment lines comprising different constructed wetland (CW) configurations: (i) partially saturated vertical subsurface flow (SVF) wetland (treatment line 1) and (ii) unsaturated vertical subsurface flow (UVF), horizontal subsurface flow (HF) and free water surface (FWS) wetlands in series (treatment line 2). Results showed important differences between the different CW configurations. The highest removal of BOD5 (81%), COD (67%), TOC (72%) and fluorescing organic matter were observed in the UVF wetland, whereas the HF and FWS wetlands were the most efficient units for total nitrogen removal (60 and 69%, respectively).The SVF wetland showed a greater performance in the reduction of total nitrogen than the UVF bed (52 vs 35%). In addition, the SVF wetland exhibited a higher removal of the EOCs caffeine (95 vs 90%), trimethoprim (99 vs 87%) and sulfamethoxazole (64 vs 4%), as opposed to DEET (34 vs 63%), whose removal was superior in the UVF unit. Sucralose was negligibly removed in all the CWs. PARAFAC analysis of fluorescence measurements revealed that the proteinaceous tryptophan-like fluorescent component was the most highly removed one in all the investigated CWs (>28%) and, particularly, in the UVF wetland (66%), whereas humic and fulvic-like components resulted recalcitrant to decomposition. Increases of fluorescence intensities were often observed for fulvic-like substances in CWs operating with saturation of the bed, and these were particularly relevant in the SVF unit. Finally, important correlations (r>0.7) between the tryptophanlike fluorescent component and the water quality parameters COD and BOD5 suggest fluorescence spectroscopy as an useful monitoring tool for water treatment efficiency in CW systems.

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“…For protocol 4, the biomass was autoclaved at 121 °C and 103 kPa for 30 minutes to ensure entire inactivation of the microbial activity [27]. For protocol 5, hydrolysis of atenolol was studied in Milli-Q water.…”

Section: Atenolol Biodegradation Experimentsmentioning

confidence: 99%

Biodegradation of atenolol by an enriched nitrifying sludge: Products and pathways

Radjenović

Yuan

et al. 2017

Chemical Engineering Journal

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Biodegradation of β-blocker atenolol was investigated using an enriched nitrifying culture at controlled ammonium concentration and without ammonium addition. Analysis of the kinetics and structural elucidation of biodegradation products showed that atenolol biodegradation was found to be linked to the activity of nitrifying bacteria in the presence of ammonium. Atenolol was degraded cometabolically by ammonia-oxidizing bacteria (AOB), likely due to a broad substrate range of ammonia monooxyenase (AMO). Four products were formed during atenolol biodegradation with ammonia oxidation, including P267 (atenolol acid) and three new products P117 (1-isopropylamino-2-propanol), P167 (1-amino-3-phenoxy-2-propanol), and an unknown product P227 with a nominal molecular mass of 227. In comparison, only P267 and P227 were identified during atenolol biodegradation without ammonia oxidation. Follow-up experiments using atenolol acid as the parent compound indicated the formation of products P117, P167 and P227 in the presence of ammonium. Based on the products identified, a tentative biodegradation pathway of atenolol is suggested, which involves two steps independent of the presence of ammonium: i) microbial amide-bond hydrolysis to carboxyl group and formation of P267 (atenolol acid) and ii) a possible formation of P227 with its unidentified structure and other two cometabolically induced reactions: iii) breakage of ether bond in the alkyl side chain and formation of P117 and iv) a minor pathway through N-dealkylation and loss of acetamide moiety from the aromatic ring, yielding P167. This study provided an important insight regarding the biotransformation pathways under different metabolic conditions.

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Enhanced sulfamethoxazole degradation through ammonia oxidizing bacteria co-metabolism and fate of transformation products

Cited by 232 publications

References 47 publications

Chronic impact of sulfamethoxazole: how does process kinetics relate to metabolic activity and composition of enriched nitrifying microbial culture?

Chronic impact of sulfamethoxazole: how does process kinetics relate to metabolic activity and composition of enriched nitrifying microbial culture?

Removal of organic carbon, nitrogen, emerging contaminants and fluorescing organic matter in different constructed wetland configurations

Biodegradation of atenolol by an enriched nitrifying sludge: Products and pathways

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