The
unique combination of DNA bases is essential for storing genetic
information. A reliable and accurate electrochemical sensor based
on a gold/palladium–polypyrrole/graphene nanocomposite (Au/Pd–PPy/GR)
has been prepared to individually and simultaneously detect guanine
(G), adenine (A), thymine (T), and cytosine (C). The bimetallic nanomaterial
(Au/Pd–PPy) is used to enhance the sensing performance of DNA
bases, and the GR could further increase the electroactive area and
amplify the electrochemical response. As expected, the electrochemical
sensor shows excellent electrocatalytic activity with well-separated
current responses at 0.704 V (G), 0.976 (A), 1.164 (T), and 1.348
V (C). The prepared sensor exhibits the linear range of 1–350
μM for G, 5–300 μM for A, 20–900 μM
for T, and 10–700 μM for C. The developed device is also
demonstrated as an excellently sensitive and highly selective sensor
to identify the four bases in calf thymus DNA, which provides the
application of electrochemical sensors in bioscience.