Enhanced therapeutic benefit of quercetin-loaded phytosome nanoparticles in ovariectomized rats

El-Fattah, Abeer I. Abd; Fathy, Mohamed; Ali, Z; El-Garawany, Abd El-Rahman A.; Mohamed, Ehsan K

doi:10.1016/j.cbi.2017.04.026

Cited by 78 publications

(48 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In comparison with the other spectra, marked changes were observed in EAPC, which confirmed the successful formation of Fungisome. The results were in accordance with the IR spectra of previously reported phytosomes (Karthivashan et al., 2016; El‐Fattah et al., 2017; Rathee & Kamboj, 2018; Telange et al., 2017).…”

Section: Resultssupporting

confidence: 92%

Antioxidant, α‐amylase and α‐glucosidase activity of various solvent fractions of I. obliquus and the preventive role of active fraction against H₂O₂ induced damage in hepatic L02 cells as fungisome

Gao

Santhanam

Xue

et al. 2020

Journal of Food Science

View full text Add to dashboard Cite

Inonotus obliquus is a traditional mushroom well known for its therapeutic value. In this study, various solvent fractions of I. obliquus were preliminarily screened for their antioxidant, α-amylase and α-glucosidase inhibition properties. To improve the drug delivery, the active fraction (ethyl acetate fraction) of I. obliquus was synthesized into fungisome (ethyl acetate phophotidyl choline complex, EAPC) and its physical parameters were assessed using Fourier transform infrared spectroscopy (FTIR), High performance liquid chromatography (HPLC), Scanning electron microscope (SEM), and ς potential analysis. Then normal human hepatic L02 cells was used to evaluate the cytotoxicity of EAPC. The results showed that EA fraction possesses significant free radical scavenging, α-amylase and α-glucosidase inhibition properties. FTIR, SEM, and HPLC analysis confirmed the fungisome formation. The particle size of EAPC was 102.80 ± 0.42 nm and the ς potential was −54.30 ± 0.61 mV. The percentage of drug entrapment efficiency was 97.13% and the drug release rates of EAPC in simulated gastric fluid and simulated intestinal fluid were 75.04 ± 0.29% and 93.03 ± 0.36%, respectively. EAPC was nontoxic to L02 cells, however it could selectively fight against the H 2 O 2 induced oxidative damage in L02 cells. This is the first study to provide scientific information to utilize the active fraction of I. obliquus as fungisome.Practical Applications: Inonotus obliquus (IO) is a traditional medicinal fungus. The extracts of IO have obvious antioxidant and hypoglycemic activities. Ethyl acetate (EA) fraction of IO was encapsulated in liposomes to form EAPC. EAPC has a sustained-release effect. It has nontoxic to L02 cells and could protect L02 cells from oxidative damage caused by hydrogen peroxide. This study could provide new ideas for the treatment of diabetes.

show abstract

Section: Resultssupporting

confidence: 92%

Antioxidant, α‐amylase and α‐glucosidase activity of various solvent fractions of I. obliquus and the preventive role of active fraction against H₂O₂ induced damage in hepatic L02 cells as fungisome

Gao

Santhanam

Xue

et al. 2020

Journal of Food Science

View full text Add to dashboard Cite

show abstract

“…Female albino rats underwent bilateral ovariectomy and were orally treated with 10 or 50 mg/kg quercetin-loaded phytosome nanoparticles for 30 days. The treatment significantly decreased bone turnover markers and increased mineral levels in the sera of the animals [30]. In another study, hydroxyapatite bioceramic microspheres loaded with quercetin were fabricated.…”

Section: Type Of Animalmentioning

confidence: 99%

“…Three-point flexural testing indicated increases in elastic and maximum radial degrees as well as elastic and maximum loads of rat femur [29]. Another group of researchers demonstrated that oral supplementation of quercetin (50 mg/kg/day) for 30 days reduced acid phosphatase (ACP) and alkaline phosphatase (ALP) activities, but increased serum calcium and phosphorus levels [30]. In another study, quercetin (2.5%) was added into the diets of female C57BL/6J mice for four weeks after undergoing bilateral ovariectomy.…”

Section: Type Of Animalmentioning

confidence: 99%

Quercetin as an Agent for Protecting the Bone: A Review of the Current Evidence

Wong

Chin

2020

IJMS

143

View full text Add to dashboard Cite

Quercetin is a flavonoid abundantly found in fruits and vegetables. It possesses a wide spectrum of biological activities, thus suggesting a role in disease prevention and health promotion. The present review aimed to uncover the bone-sparing effects of quercetin and its mechanism of action. Animal studies have found that the action of quercetin on bone is largely protective, with a small number of studies reporting negative outcomes. Quercetin was shown to inhibit RANKL-mediated osteoclastogenesis, osteoblast apoptosis, oxidative stress and inflammatory response while promoting osteogenesis, angiogenesis, antioxidant expression, adipocyte apoptosis and osteoclast apoptosis. The possible underlying mechanisms involved are regulation of Wnt, NF-κB, Nrf2, SMAD-dependent, and intrinsic and extrinsic apoptotic pathways. On the other hand, quercetin was shown to exert complex and competing actions on the MAPK signalling pathway to orchestrate bone metabolism, resulting in both stimulatory and inhibitory effects on bone in parallel. The overall interaction is believed to result in a positive effect on bone. Considering the important contributions of quercetin in regulating bone homeostasis, it may be considered an economical and promising agent for improving bone health. The documented preclinical findings await further validation from human clinical trials.

show abstract

“…Morphology Vesicles morphology of phytosomes was observed by Transmission Electron Microscopy (TEM). [23][24][25][26][27] Sample was prepared at room temperature using 1% phosphotungstate acid (pH 6.0) as a negative staining agent. 24 After drying the specimen was seen under a microscope with a magnification of 7,000 -71,000 times.…”

Section: Phytosomes Characterizationmentioning

confidence: 99%

“…The particle size distribution and zeta potential (ZP) of prepared phytosomes were determined using the nano zetasizer (Malvern, UK). 16,24,26,28 Phytosomes were diluted with double distillate water and the sample was transferred to disposable cell to perform the measurement.…”

Section: Fourier Transformation Infrared Spectroscopy (Ftir)mentioning

confidence: 99%

Formulation and Characterization of Bitter Melon Extract (Momordica charantia) Loaded Phytosomes

Sasongko

Surini²,

Saputri³

2019

View full text Add to dashboard Cite

Objective: Phytosomes are a novel drug delivery system that offers better absorption and bioavailability for extract or phytoconstituents. The aim of this study was developing bitter melon extract load phytosomes with appropriate characteristics for transdermal delivery. Methods: Three formulas were developed, F1, F2 and F3 with weight ratios of extract and phosphatidylcholine were 1: 1, 1: 2 and 1: 3, respectively. Bitter melon fruit was extracted using a maceration method and the marker compounds were determined by high performance liquid chromatography (HPLC) method. Phytosomes were prepared using thin layer method and then characterized, in terms of morphology, particle size distribution, zeta potential and entrapment efficiency. Results: The results of pytosomes characterization reveals that the F3 was the optimal formula. It has a spherical shape, particle size (D V-mean) was 282.3 ± 16.4 nm, zeta potential value at-39.2 ± 0.14 mV and entrapment efficiency of 90.06 ± 1.07 %. Conclusion: Bitter melon extract loaded phytosomes with a weight ratio of extract and phosphatidylcholine 1:3 (F3) was selected as an optimal formula with appropriate characteristics for transdermal delivery.

show abstract

Enhanced therapeutic benefit of quercetin-loaded phytosome nanoparticles in ovariectomized rats

Cited by 78 publications

References 37 publications

Antioxidant, α‐amylase and α‐glucosidase activity of various solvent fractions of I. obliquus and the preventive role of active fraction against H₂O₂ induced damage in hepatic L02 cells as fungisome

Antioxidant, α‐amylase and α‐glucosidase activity of various solvent fractions of I. obliquus and the preventive role of active fraction against H₂O₂ induced damage in hepatic L02 cells as fungisome

Quercetin as an Agent for Protecting the Bone: A Review of the Current Evidence

Formulation and Characterization of Bitter Melon Extract (Momordica charantia) Loaded Phytosomes

Contact Info

Product

Resources

About

Enhanced therapeutic benefit of quercetin-loaded phytosome nanoparticles in ovariectomized rats

Cited by 78 publications

References 37 publications

Antioxidant, α‐amylase and α‐glucosidase activity of various solvent fractions of I. obliquus and the preventive role of active fraction against H2O2 induced damage in hepatic L02 cells as fungisome

Antioxidant, α‐amylase and α‐glucosidase activity of various solvent fractions of I. obliquus and the preventive role of active fraction against H2O2 induced damage in hepatic L02 cells as fungisome

Quercetin as an Agent for Protecting the Bone: A Review of the Current Evidence

Formulation and Characterization of Bitter Melon Extract (Momordica charantia) Loaded Phytosomes

Contact Info

Product

Resources

About

Antioxidant, α‐amylase and α‐glucosidase activity of various solvent fractions of I. obliquus and the preventive role of active fraction against H₂O₂ induced damage in hepatic L02 cells as fungisome

Antioxidant, α‐amylase and α‐glucosidase activity of various solvent fractions of I. obliquus and the preventive role of active fraction against H₂O₂ induced damage in hepatic L02 cells as fungisome