Enhanced tissue adhesiveness of injectable gelatin hydrogels through dual catalytic activity of horseradish peroxidase

Thi, Thai Thanh Hoang; Lee, Yunki; Ryu, Seung Bae; Nguyen, Dai Hai

doi:10.1002/bip.23077

Cited by 32 publications

(15 citation statements)

References 30 publications

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“…Thi et al fabricated a gelatin-based hydrogel with dual cross-linking functionality by mixing hydroxyphenyl propionic acid-modified gelatin (GH) with thiolated gelatin (GS). 284 GH was cross-linked with HRP/H 2 O 2 , and the adhesive strength of the hydrogel increased 6-fold upon addition of GS due to the formation of disulfide cross-links within the hydrogel and at the hydrogel-tissue interface. 284 Engineered polypeptides have also been designed to form hydrogels via disulfide bonds.…”

Section: Disulfide Cross-linkingmentioning

confidence: 99%

“…284 GH was cross-linked with HRP/H 2 O 2 , and the adhesive strength of the hydrogel increased 6-fold upon addition of GS due to the formation of disulfide cross-links within the hydrogel and at the hydrogel-tissue interface. 284 Engineered polypeptides have also been designed to form hydrogels via disulfide bonds. Shen et al designed am artificial protein cross-linked by leucine zipper domains.…”

Section: Disulfide Cross-linkingmentioning

confidence: 99%

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Chemically Modified Biopolymers for the Formation of Biomedical Hydrogels

2020

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Biopolymers are natural polymers sourced from plants and animals, which include a variety of polysaccharides and polypeptides. The inclusion of biopolymers into biomedical hydrogels is of great interest because of their inherent biochemical and biophysical properties, such as cellular adhesion, degradation, and viscoelasticity. The objective of this Review is to provide a detailed overview of the design and development of biopolymer hydrogels for biomedical applications, with an emphasis on biopolymer chemical modifications and cross-linking methods. First, the fundamentals of biopolymers and chemical conjugation methods to introduce cross-linking groups are described. Cross-linking methods to form biopolymer networks are then discussed in detail, including (i) covalent cross-linking (e.g., free radical chain polymerization, click cross-linking, cross-linking due to oxidation of phenolic groups), (ii) dynamic covalent cross-linking (e.g., Schiff base formation, disulfide formation, reversible Diels–Alder reactions), and (iii) physical cross-linking (e.g., guest–host interactions, hydrogen bonding, metal–ligand coordination, grafted biopolymers). Finally, recent advances in the use of chemically modified biopolymer hydrogels for the biofabrication of tissue scaffolds, therapeutic delivery, tissue adhesives and sealants, as well as the formation of interpenetrating network biopolymer hydrogels, are highlighted.

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Section: Disulfide Cross-linkingmentioning

confidence: 99%

Section: Disulfide Cross-linkingmentioning

confidence: 99%

Chemically Modified Biopolymers for the Formation of Biomedical Hydrogels

2020

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“…Similarly, other hydrogels were formed; the specific formulations and corresponding code are presented in Table 3. The time when the mixtures stopped flowing was recorded as the gelation time [30,31]. Each sample was triplicated to calculate the average value and standard deviation.…”

Section: Hydrogel Formation and Characterizationmentioning

confidence: 99%

Supramolecular Gels Incorporating Cordyline terminalis Leaf Extract as a Polyphenol Release Scaffold for Biomedical Applications

Thi

Tran

Thi

et al. 2021

IJMS

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Cordyline terminalis leaf extract (aqCT) possesses abundant polyphenols and other bioactive compounds, which are encapsulated in gelatin–polyethylene glycol–tyramine (GPT)/alpha-cyclodextrin (α-CD) gels to form the additional functional materials for biomedical applications. In this study, the gel compositions are optimized, and the GPT/α-CD ratios equal to or less than one half for solidification are found. The gelation time varies from 40.7 min to 5.0 h depending on the increase in GPT/α-CD ratios and aqCT amount. The aqCT extract disturbs the hydrogen bonding and host–guest inclusion of GPT/α-CD gel networks, postponing the gelation. Scanning electron microscope observation shows that all gels with or without aqCT possess a microarchitecture and porosity. GPT/α-CD/aqCT gels could release polyphenols from 110 to 350 nmol/mL at the first hour and sustainably from 5.5 to 20.2 nmol/mL for the following hours, which is controlled by feeding the aqCT amount and gel properties. GPT/α-CD/aqCT gels achieved significant antioxidant activity through a 100% scavenging DPPH radical. In addition, all gels are non-cytotoxic with a cell viability more than 85%. Especially, the GPT3.75α-CD10.5aqCT gels with aqCT amount of 3.1–12.5 mg/mL immensely enhanced the cell proliferation of GPT3.75α-CD10.5 gel without extract. These results suggest that the inherent bioactivities of aqCT endowed the resulting GPT/α-CD/aqCT gels with effective antioxidant and high biocompatibility, and natural polyphenols sustainably release a unique platform for a drug delivery system or other biomedical applications.

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“…Recently, there has been growing interest in creating in situ cross-linkable hydrogels through enzymatic reaction because of the hydrogel formation in physiological conditions and substrate-specific conjugations. Several types of enzyme-mediated cross-linkable hydrogels have been developed using several enzymes [e.g., horseradish peroxidase (HRP), transglutaminase, tyrosinase, lysyl oxidase, and laccase (Lac)] [15][16][17][34][35][36][37][38][39]. Specifically, HRP-mediated cross-linking reactions have attracted attention as a promising chemical cross-linking method because of the biocompatibility and easily controllable physicochemical and biological properties of the hydrogels (e.g., gelation kinetics, mechanical strength, and degradation behaviors) [40,41].…”

Section: Strategies To Fabricate In Situ Cross-linkable Hydrogelsmentioning

confidence: 99%

In Situ Cross-Linkable Hydrogels as a Dynamic Matrix for Tissue Regenerative Medicine

Park

Парк

2018

Tissue Eng Regen Med

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BACKGROUND: Polymeric hydrogels are extensively used as promising biomaterials in a broad range of biomedical applications, including tissue engineering, regenerative medicine, and drug delivery. These materials have advantages such as structural similarity to the native extracellular matrix (ECM), multi-tunable physicochemical and biological properties, and biocompatibility. METHODS: In situ forming hydrogels show a phase transition from a solution to a gel state through various physical and chemical cross-linking reactions. These advanced hydrogel materials have been widely used for tissue regenerative medicine because of the ease of encapsulating therapeutic agents, such as cells, drugs, proteins, and genes. RESULTS: With advances in biomaterials engineering, these hydrogel materials have been utilized as either artificial cellular microenvironments to create engineered tissue constructs or as bioactive acellular matrices to stimulate the native ECM for enhanced tissue regeneration and restoration. CONCLUSION: In this review, we discuss the use of in situ cross-linkable hydrogels in tissue engineering and regenerative medicine applications. In particular, we focus on emerging technologies as a powerful therapeutic tool for tissue regenerative medicine applications.

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Enhanced tissue adhesiveness of injectable gelatin hydrogels through dual catalytic activity of horseradish peroxidase

Cited by 32 publications

References 30 publications

Chemically Modified Biopolymers for the Formation of Biomedical Hydrogels

Chemically Modified Biopolymers for the Formation of Biomedical Hydrogels

Supramolecular Gels Incorporating Cordyline terminalis Leaf Extract as a Polyphenol Release Scaffold for Biomedical Applications

In Situ Cross-Linkable Hydrogels as a Dynamic Matrix for Tissue Regenerative Medicine

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