Enhanced Tolerance against a Fungal Pathogen and Insect Resistance in Transgenic Tobacco Plants Overexpressing an Endochitinase Gene from Serratia marcescens

Navarro-González, Samantha Sarai; Ramírez-Trujillo, José Augusto; Peña-Chora, Guadalupe; Gaytán, Paul; Roldán‐Salgado, Abigail; Corzo, Gerardo; Lina-García, Laura Patricia; Hernández-Velázquez, Víctor Manuel; Suárez-Rodríguez, Ramón

doi:10.3390/ijms20143482

Cited by 21 publications

(11 citation statements)

References 56 publications

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“…This confirmed that CpCHI4 could be a potent candidate gene to tackle H. armigera damage on crops corroborating with the demonstration of utilization of chitinase genes from various sources to control lepidopteron pests 13,48 . Furthermore, such genes of biochemical relevance to both insects as well as plants can be useful for resistance management, which otherwise has been a concern.…”

Section: Resultssupporting

confidence: 72%

“…Alternatively, genes encoding for proteins with different defense functions such as digestive enzyme inhibitors 10 and proteins disrupting physical structures 11–13 in insect larvae have been successfully utilized for the development of insect resistance in plants. Among them, chitinase genes encoding enzymes that hydrolyze glycoside bonds in the chitin polymer have been well‐documented for insecticidal activity 13 . Chitin is known to be an important structural component of insect periplasmic membrane (PM) and is responsible for curtailing the entry of toxic chemicals, bacteria and viruses that infect insect cells 14 .…”

Section: Introductionmentioning

confidence: 99%

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Characterization and in planta validation of a CHI4 chitinase from Cajanus platycarpus (Benth.) Maesen for its efficacy against pod borer, Helicoverpa armigera (Hübner)

Rathinam

Marimuthu

Tyagi

et al. 2021

Pest Management Science

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BACKGROUND Pigeonpea, Cajanus cajan is one of the economically important legume food crops and a major source of dietary proteins. Management of pod borer, Helicoverpa armigera has been prominent among crop improvement programs. Lack of resistance sources in the cultivated germplasm and crossing incompatibility with pod borer‐resistant wild relatives have prompted biotechnological interventions. Identification and exploitation of genes from pigeonpea wild relatives in host plant resistance towards the pod borer assumes pertinence. Dynamic transcriptome analysis of the wild relative vis a vis cultivated pigeonpea identified a CHI4 chitinase as one of the putative insect resistance genes. RESULTS The study presents variations in important amino acids in CHI4 chitinases from C. cajan and its wild relative C. platycarpus. Comparative protein modeling and docking analysis of the two proteins demonstrated differences in substrate binding efficacy of the chitinase from C. platycarpus which resulted in a minimum binding energy of −8.7 kcal mol−1. Furthermore, we successfully evaluated the insecticidal activity of the chitinase from C. platycarpus against H. armigera challenge through heterologous expression in tobacco. Molecular characterization of transgenic plants confirmed that their efficacy against H. armigera was a result of the integration of CHI4 from C. platycarpus. CONCLUSION Docking analysis demonstrated effective substrate interaction as a possible reason for efficacy against pod borer in the chitinase from C. platycarpus. This was authenticated by successful overexpression and bioefficacy assessment against H. armigera in tobacco. The CHI4 gene from C. platycarpus can be useful in the mitigation of H. armegira in pigeonpea as well as in other crops. © 2021 Society of Chemical Industry

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Section: Resultssupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Characterization and in planta validation of a CHI4 chitinase from Cajanus platycarpus (Benth.) Maesen for its efficacy against pod borer, Helicoverpa armigera (Hübner)

Rathinam

Marimuthu

Tyagi

et al. 2021

Pest Management Science

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“…Hence, biological control strategies tend to provide a plant protection solution that is environmentally friendly, ecologically viable and has great potential to promote sustainable agriculture. Microorganisms in the genera Bacillus, Pseudomonas, Serratia and fungi which belong to the genera Trichoderma are the common biocontrol agents used in the disease control of fungal pathogens [30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Biological Control of Celery Powdery Mildew Disease Caused by Erysiphe heraclei DC In Vitro and In Vivo Conditions

Ahmed

Seleiman

Al-Saif

et al. 2021

Plants

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The present study aimed to investigate the potentiality of certain biocontrol agents, namely Bacillus subtilis, B. pumilus, B. megaterium, Pseudomonas fluorescens, Serratia marcescens, Trichoderma album, T. harzianum and T. viride, as well as the synthetic fungicide difenoconazole to control celery powdery mildew caused by Erysiphe heraclei DC, in vitro (against conidia germination and germ tube length of E. heraclei) and in vivo (against disease severity and AUDPC). In vitro, it was found that the antifungal activity of the tested biocontrol agents significantly reduced the germination percentage of the conidia and germ tube length of the pathogen. The reduction in conidia germination ranged between 88.2% and 59.6% as a result of the treatment with B. subtilis and T. album, respectively compared with 97.1% by the synthetic fungicide difenoconazole. Moreover, the fungicide achieved the highest reduction in germ tube length (92.5%) followed by B. megaterium (82.0%), while T. album was the least effective (62.8%). Spraying celery plants with the tested biocontrol agents in the greenhouse significantly reduced powdery mildew severity, as well as the area under the disease progress curve (AUDPC), after 7, 14, 21 and 28 days of application. In this regard, B. subtilis was the most efficient followed by B. pumilus, S. marcescens and B. megaterium, with 80.1, 74.4, 73.2 and 70.5% reductions in disease severity, respectively. In AUDPC, reductions of those microorganisms were 285.3, 380.9, 396.7 and 431.8, respectively, compared to 1539.1 in the control treatment. On the other hand, the fungicide difenoconazole achieved maximum efficacy in reducing disease severity (84.7%) and lowest AUDPC (219.3) compared to the other treatments. In the field, all the applied biocontrol agents showed high efficiency in suppressing powdery mildew on celery plants, with a significant improvement in growth and yield characteristics. In addition, they caused an increase in the concentration of leaf pigments, and the activities of defense-related enzymes such as peroxidase (PO) and polyphenol oxidase (PPO) and total phenol content (TPC). In conclusion, the results showed the possibility of using tested biocontrol agents as eco-friendly alternatives to protect celery plants against powdery mildew.

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“…Due to these inherent chemical and physical characteristics of chitin its commercial applications in the native form are currently rather limited. Commonly native chitin is utilised as fertilizer in agriculture, as it contains nitrogen, or as an inducer of plant defence mechanisms, enhancing the resistance against fungi by the promotion of chitinases [21][22][23]. Furthermore, chitin is employed as a food additive [24] as well as a packaging material for affinity chromatography columns to purify proteins with a carbohydrate-binding domain, or as sorbent to pre-concentrate phenol and chlorophenols by means of solid phase extraction, as validated by HPLC [25,26].…”

Section: Chitinmentioning

confidence: 99%

Enzymatic Modification of Native Chitin and Conversion to Specialty Chemical Products

Arnold

Brück²,

Garbe

et al. 2020

Marine Drugs

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Chitin is one of the most abundant biomolecules on earth, occurring in crustacean shells and cell walls of fungi. While the polysaccharide is threatening to pollute coastal ecosystems in the form of accumulating shell-waste, it has the potential to be converted into highly profitable derivatives with applications in medicine, biotechnology, and wastewater treatment, among others. Traditionally this is still mostly done by the employment of aggressive chemicals, yielding low quality while producing toxic by-products. In the last decades, the enzymatic conversion of chitin has been on the rise, albeit still not on the same level of cost-effectiveness compared to the traditional methods due to its multi-step character. Another severe drawback of the biotechnological approach is the highly ordered structure of chitin, which renders it nigh impossible for most glycosidic hydrolases to act upon. So far, only the Auxiliary Activity 10 family (AA10), including lytic polysaccharide monooxygenases (LPMOs), is known to hydrolyse native recalcitrant chitin, which spares the expensive first step of chemical or mechanical pre-treatment to enlarge the substrate surface. The main advantages of enzymatic conversion of chitin over conventional chemical methods are the biocompability and, more strikingly, the higher product specificity, product quality, and yield of the process. Products with a higher Mw due to no unspecific depolymerisation besides an exactly defined degree and pattern of acetylation can be yielded. This provides a new toolset of thousands of new chitin and chitosan derivatives, as the physio-chemical properties can be modified according to the desired application. This review aims to provide an overview of the biotechnological tools currently at hand, as well as challenges and crucial steps to achieve the long-term goal of enzymatic conversion of native chitin into specialty chemical products.

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Enhanced Tolerance against a Fungal Pathogen and Insect Resistance in Transgenic Tobacco Plants Overexpressing an Endochitinase Gene from Serratia marcescens

Cited by 21 publications

References 56 publications

Characterization and in planta validation of a CHI4 chitinase from Cajanus platycarpus (Benth.) Maesen for its efficacy against pod borer, Helicoverpa armigera (Hübner)

Characterization and in planta validation of a CHI4 chitinase from Cajanus platycarpus (Benth.) Maesen for its efficacy against pod borer, Helicoverpa armigera (Hübner)

Biological Control of Celery Powdery Mildew Disease Caused by Erysiphe heraclei DC In Vitro and In Vivo Conditions

Enzymatic Modification of Native Chitin and Conversion to Specialty Chemical Products

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