Enhanced tolerance of transgenic sweetpotato plants that express both CuZnSOD and APX in chloroplasts to methyl viologen-mediated oxidative stress and chilling

Tian

J. Zhejiang Univ. Sci. B

et al. 2013

An 888-bp full-length ascorbate peroxidase (APX) complementary DNA (cDNA) gene was cloned from Anthurium andraeanum, and designated as AnAPX. It contains a 110-bp 5′-noncoding region, a 28-bp 3′-noncoding region, and a 750-bp open reading frame (ORF). This protein is hydrophilic with an aliphatic index of 81.64 and its structure consisting of α-helixes, β-turns, and random coils. The AnAPX protein showed 93%, 87%, 87%, 87%, and 86% similarities to the APX homologs from Zantedeschia aethiopica, Vitis pseudoreticulata, Gossypium hirsutum, Elaeis guineensis, and Zea mays, respectively. AnAPX gene transcript was measured non-significantly in roots, stems, leaves, spathes, and spadices by real-time polymerase chain reaction (RT-PCR) analysis. Interestingly, this gene expression was remarkably up-regulated in response to a cold stress under 6 °C, implying that AnAPX might play an important role in A. andraeanum tolerance to cold stress. To confirm this function we overexpressed AnAPX in tobacco plants by transformation with an AnAPX expression construct driven by CaMV 35S promoter. The transformed tobacco seedlings under 4 °C showed less electrolyte leakage (EL) and malondialdehyde (MDA) content than the control. The content of MDA was correlated with chilling tolerance in these transgenic plants. These results show that AnAPX can prevent the chilling challenged plant from cell membrane damage and ultimately enhance the plant cold tolerance.

Section: Discussionmentioning

confidence: 89%

Cloning and functional analysis of a novel ascorbate peroxidase (APX) gene from Anthurium andraeanum

Tian

J. Zhejiang Univ. Sci. B

et al. 2013

“…Kwon et al [234] demonstrated that simultaneous expression of Cu/Zn-SOD and APX genes in tobacco chloroplasts enhanced tolerance to methyl viologen (MV) stress compared to expression of either of these genes alone. Similarly, enhanced tolerance to multiple environmental stresses has been developed by simultaneous overexpression of the genes of SOD and APX in the chloroplasts [235,236], SOD and CAT in cytosol [231] and SOD and GR in cytosol [233]. Further, simultaneous expression of multiple antioxidant enzymes, such as Cu/Zn-SOD, APX, and DHAR, in chloroplasts has shown to be more effective than single or double expression for developing transgenic plants with enhanced tolerance to multiple environmental stresses [26].…”

Section: (4) Glutathione Reductase (Gr)mentioning

confidence: 99%

Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions

et al. 2012

Reactive oxygen species (ROS) are produced as a normal product of plant cellular metabolism. Various environmental stresses lead to excessive production of ROS causing progressive oxidative damage and ultimately cell death. Despite their destructive activity, they are well-described second messengers in a variety of cellular processes, including conferment of tolerance to various environmental stresses. Whether ROS would serve as signaling molecules or could cause oxidative damage to the tissues depends on the delicate equilibrium between ROS production, and their scavenging. Efficient scavenging of ROS produced during various environmental stresses requires the action of several nonenzymatic as well as enzymatic antioxidants present in the tissues. In this paper, we describe the generation, sites of production and role of ROS as messenger molecules as well as inducers of oxidative damage. Further, the antioxidative defense mechanisms operating in the cells for scavenging of ROS overproduced under various stressful conditions of the environment have been discussed in detail.

Journal of Plant Biotechnology

“…This study, also, the integration of two transgenes in progeny obtained from the crossing between the independently transformed petunia lines, SOD2-2-1-1-35 and NDPK2-5-3 obtained from petunia pure line Wongyo A2-36, was analyzed using PCR. The number of plants with two genes (SOD2 and NDPK2) was 23 out of 56 seedlings of the progeny (Table 2 and Kim et al 1986;Kwon et al 2002;Lim et al 2007;Moon et al 2003;Suntres 2002;Tang et al 2008). In case the transgenic plant is less damaged after treatment with MV than wild type, it will be concluded that the resistance of transgenic plant to oxidative stress is more increased than that of wild type plant.…”

Section: Resultsmentioning

confidence: 96%

“…Recent developments in plant genetic engineering are aimed at increasing resistance by integration of multiple transgenes into plants genome (multiple-transgene-stacking) and coordinated expression of these transgenes in transgenic plants (François et al 2002). Also, there were recent reports on transgenic plants that are more resistant to abiotic stess through the introduction of two genes (SOD and APX genes) (Kwon et al 2002;Lim et al 2007). …”

Section: Introductionmentioning

confidence: 99%

Increase of resistance to oxidative stress induced by methyl viologen in progeny from a cross between two transgenic Petunia lines with NDPK and SOD genes

Lee¹,

Lee²,

Kim³

2011

This study was conducted to investigate how to enhance resistance to oxidative stress in petunia progeny obtained by a crossing between transgenic plants, MnSOD (SOD2) (T 4 ) and NDPK2 (T 2 ), to develop transgenic petunia much more resistant to environmental stress. At the treatment of MV 200 μM, the progeny was significantly less damaged than its parental plants (SOD2-or NDPK2-transgenic lines) as well as wild type plants, implying its resistance to oxidative stress was enhanced compare to that of SOD2-or NDPK2-transgenic plants. In an expression of 11 quantitative traits, the progeny remained similar to control plants, although it infrequently displayed slightly longer or wider than either parental or wild type plants. In the expression of 6 qualitative traits, there was no significant difference between parental or non-transgenic control plants.