Enhanced tolerance to drought stress in transgenic tobacco plants overexpressing VTE1 for increased tocopherol production from Arabidopsis thaliana

Liu, Xiaoli; Hua, Xuejun; Guo, Jian-Wei; Qi, Dongmei; Wang, Limin; Liu, Zhipeng; Jin, Zhen; Chen, Shuangyan; Liu, Gongshe

doi:10.1007/s10529-008-9672-y

Cited by 139 publications

(71 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…LsTC was clustered with TC from Helianthus annuus, since they both belonged to Compositae species. An earlier study has shown that TC could endow plants with enhanced tolerance to drought stress, along with higher tocopherol content and lower lipid peroxidation levels (Liu et al, 2008). There is enough evidence that tocopherol content correlates positively with tolerance to lo� temperature, �ater �eficit or salt stress in �ifferent plants (Yama�uchi��hinozaki and Shinozaki, 1994;Munne-Bosch et al, 1999;Guo et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and characterization of a tocopherol cyclase gene from Lactuca sativa (Asteraceae)

Tang¹,

Ren²,

Zhang³

et al. 2011

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Tocopherol cyclase is a rate-limiting enzyme involved in tocopherol biosynthesis. The full-length cDNA encoding tocopherol cyclase (designated as LsTC) was cloned from lettuce (Lactuca sativa) for the first time by rapid amplification of cDNA ends (RACE) and characterized by means of quantitative RT-PCR. The full-length cDNA of LsTC was 1675 bp, with an open reading frame of 1521 bp, encoding a tocopherol cyclase protein of 506 amino acids, with a calculated molecular mass of 56.76 kD and an isoelectric point of 6.49. Comparative analysis revealed that LsTC has a close similarity with tocopherol cyclases from other plant species. Bioinformatic analysis indicated that LsTC shares a common evolutionary origin based on sequence and has the closest relationship to tocopherol cyclase from Helianthus annuus. Quantitative RT-PCR analysis suggested that expression of LsTC is induced and strengthened by oxidative stresses, such as strong light and drought. This cloning and characterization of LsTC will be helpful for further understanding of its role in the tocopherol biosynthesis pathway and provide a candidate gene for metabolic engineering of vitamin E.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular cloning and characterization of a tocopherol cyclase gene from Lactuca sativa (Asteraceae)

Tang¹,

Ren²,

Zhang³

et al. 2011

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…The change in cell membrane permeability and the degree of cell destruction caused by the stresses can be reflected directly through the change in electrolyte permeability, which was measured by the rate of relative electrolyte leakage (82). Moreover, enhanced tocopherol accumulation also occurs in response to a variety of abiotic stresses including high light, drought, high salinity, and cold and may provide an additional line of protection from oxidative damage (38,(83)(84)(85). In this study, leaves from the freeze-stressed BSMV PAP6-liketreated plants had distinctly more severe droop and wilt than the freeze-stressed viral controls.…”

Section: Identification Of Winter-responsive Proteins In Bread Wheatmentioning

confidence: 99%

Identification of Winter-Responsive Proteins in Bread Wheat Using Proteomics Analysis and Virus-Induced Gene Silencing (VIGS)

Zhang

Huo

Zhang

et al. 2016

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

Proteomic approaches were applied to identify protein spots involved in cold responses in wheat. By comparing the differentially accumulated proteins from two cultivars (UC1110 and PI 610750) and their derivatives, as well as the F 10 recombinant inbred line population differing in cold-tolerance, a total of 20 common protein spots representing 16 unique proteins were successfully identified using 2-DE method. Of these, 14 spots had significantly enhanced abundance in the cold-sensitive parental cultivar UC1110 and its 20 descendant lines when compared with the cold-tolerant parental cultivar PI 610750 and its 20 descendant lines. Six protein spots with reduced abundance were also detected. The identified protein spots are involved in stress/defense, carbohydrate metabolism, protein metabolism, nitrogen metabolism, energy metabolism, and photosynthesis. The 20 differentially expressed protein spots were chosen for quantitative real-time polymerase chain reaction (qRT-PCR) to investigate expression changes at the RNA level. The results indicated that the transcriptional expression patterns of 11 genes were consistent with their protein expression models. Among the three unknown proteins, Spot 20 (PAP6-like) showed high sequence similarities with PAP6. qRT-PCR results implied that cold and salt stresses increased the expression of PAP6-like in wheat leaves. Furthermore, VIGS (virus-induced gene silencing)-treated plants generated for PAP6-like were subjected to freezing stress, these plants had more serious droop and wilt, an increased rate of relative electrolyte leakage, reduced relative water content (RWC) and decreased tocopherol levels when compared with viral control plants. However, the plants that were silenced for the other two unknown proteins had no significant differences in comparison to the BSMV 0 -inoculated plants under freezing conditions. These results indicate that PAP6-like possibly plays an important role in conferring cold tolerance in wheat. Molecular & Cellular

show abstract

“…In addition, Guo et al (2008) found that Triticum aestivum ubiquitin 2 (Ta-Ub2) accelerated the germination of seedlings under water deficit conditions and then improved the CO 2 assimilation rate of transgenic plants under both drought and non-drought conditions [8] Furthermore, a 3.8-fold higher level of ascorbate peroxidase (APX5) activity enhanced the net photosynthetic rate, and reduced toxicity of H 2 O 2 in transgenic tobacco plants [9]. However, tocopherol cyclase (VTE1) decreased lipid peroxidation, electrolyte leakage and H 2 O 2 content, and increased chlorophyll and tocopherol content in transgenic tobacco as compared to wild type plants in response to drought stress [10]. In addition, the LTP gene family, which includes at least five tightly regulated and differentially expressed genes, was linked to increased accumulation of cuticular wax in leaves of tree tobacco in response to periodic drought [11].…”

Section: Introductionmentioning

confidence: 99%

Analysis of global gene expression profiles in tobacco roots under drought stress

et al. 2015

View full text Add to dashboard Cite

were grouped into 43 functional categories involved in seven significant pathways. The most enriched categories were those that were populated by transcripts involved in metabolism, signal transduction and cellular transport. Many genes and/or biological pathways were found to be common among the three libraries, for example, genes participating in transport, stress response, auxin transport and signaling, etc. Next, the expression patterns of 12 genes were assessed with quantitative real-time PCR, the results of which agreed with the Solexa analysis. In conclusion, we revealed complex changes in the transcriptome during tobacco root development related to drought resistance, and provided a comprehensive set of data that is essential to understanding the molecular regulatory mechanisms involved. These data may prove valuable in future studies of the molecular mechanisms regulating root development in response to drought stress in tobacco and other plants. IntroductionTobacco is an important cash crop in China as well as around the world, being cultivated in more than 100 countries. In addition, a recent study investigated the use of tobacco as a potential biofuel crop [1]. It is also recognized as an established model plant for physiological and genetic studies. Drought is one of the major abiotic stresses affecting crop growth, development, yield and quality, and in extreme cases, causes crop failure. In response to environmental extremes, plants have adapted several mechanisms allowing them to survive adverse conditions. Although much research has been dedicated to elucidating plant gene expression during exposure to dry conditions, the mechanisms underlying the regulation of gene expression largely remain unknown. DOI 10.1515/biol-2015-0035 Received November 11, 2014 accepted March 8, 2015 Abstract: Tobacco (Nicotiana tabacum L.) is an economically important and relatively drought-tolerant crop grown around the world. However, the molecular regulatory mechanisms involved in tobacco root development in response to drought stress are not wellknown. To gain insight into the transcriptome dynamics associated with drought resistance, genome-wide gene expression profiling of roots from a tobacco cultivar (Honghua Dajinyuan, a major flue-cured tobacco cultivar in Southwest China) under 20% PEG6000 treatment for 0, 6 h and 48 h were conducted using Solexa sequencing (Illumina Inc., San Diego, CA, USA). Over five million tags were generated from tobacco roots, including 229,344, 221,248 and 242,065 clean tags in three libraries, respectively. The most differentially expressed tags, with either log2FC > 2.0 for up-regulated genes or log2FC < -2.0 for down regulated genes (p < 0.001), were analyzed further. In comparison to the control, 1476 up-regulated and 1574 down-regulated differentially expressed genes (DEGs) were identified, except for unknown transcripts, which

show abstract

Enhanced tolerance to drought stress in transgenic tobacco plants overexpressing VTE1 for increased tocopherol production from Arabidopsis thaliana

Cited by 139 publications

References 16 publications

Molecular cloning and characterization of a tocopherol cyclase gene from Lactuca sativa (Asteraceae)

Molecular cloning and characterization of a tocopherol cyclase gene from Lactuca sativa (Asteraceae)

Identification of Winter-Responsive Proteins in Bread Wheat Using Proteomics Analysis and Virus-Induced Gene Silencing (VIGS)

Analysis of global gene expression profiles in tobacco roots under drought stress

Contact Info

Product

Resources

About