Enhanced Type X Collagen Expression in the Extruded Nucleus Pulposus of the Chondrodystrophoid Dog

Itoh, Hisanori; Asou, Yoshinori; Hara, Yasushi; Haro, Hirotaka; Shinomiya, Kenichi; Tagawa, Masahiro

doi:10.1292/jvms.70.37

Cited by 9 publications

(7 citation statements)

References 22 publications

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“…Besides showing similar pathological changes, canines are also the only animals diagnosed and treated both medically and surgically as humans for their disc degeneration [ 46 ]. As such, canine spinal models have been previously used to investigate disc degeneration and to develop surgical treatments such as spinal fusion [ 47 , 48 ] and regenerative intervention [ 38 ]. This is especially true of beagles, which are frequently used for biological approaches aimed at disc regeneration.…”

Section: Discussionmentioning

confidence: 99%

Total disc replacement using tissue-engineered intervertebral discs in the canine cervical spine

et al. 2017

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The most common reason that adults in the United States see their physician is lower back or neck pain secondary to degenerative disc disease. To date, approaches to treat degenerative disc disease are confined to purely mechanical devices designed to either eliminate or enable flexibility of the diseased motion segment. Tissue engineered intervertebral discs (TE-IVDs) have been proposed as an alternative approach and have shown promise in replacing native IVD in the rodent tail spine. Here we demonstrate the efficacy of our TE-IVDs in the canine cervical spine. TE-IVD components were constructed using adult canine annulus fibrosis and nucleus pulposus cells seeded into collagen and alginate hydrogels, respectively. Seeded gels were formed into a single disc unit using molds designed from the geometry of the canine spine. Skeletally mature beagles underwent discectomy with whole IVD resection at levels between C3/4 and C6/7, and were then divided into two groups that received only discectomy or discectomy followed by implantation of TE-IVD. Stably implanted TE-IVDs demonstrated significant retention of disc height and physiological hydration compared to discectomy control. Both 4-week and 16-week histological assessments demonstrated chondrocytic cells surrounded by proteoglycan-rich matrices in the NP and by fibrocartilaginous matrices in the AF portions of implanted TE-IVDs. Integration into host tissue was confirmed over 16 weeks without any signs of immune reaction. Despite the significant biomechanical demands of the beagle cervical spine, our stably implanted TE-IVDs maintained their position, structure and hydration as well as disc height over 16 weeks in vivo.

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Section: Discussionmentioning

confidence: 99%

Total disc replacement using tissue-engineered intervertebral discs in the canine cervical spine

et al. 2017

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“…Type X collagen is often detected in the calcified tissues, 30,31 revealing a close relationship between type X collagen and calcification. A large amount of type X collagen is detected in chondrocytes in degenerative intervertebral disc 32 . Thus, type X collagen is critical for IVDD and repair.…”

Section: Discussionmentioning

confidence: 99%

Calcitonin inhibits intervertebral disc degeneration by regulating protein kinase C

Cheng

et al. 2020

J Cellular Molecular Medi

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Intervertebral disc degeneration (IVDD) is the most critical factor that causes low back pain. Molecular biotherapy is a fundamental strategy for IVDD treatment. Calcitonin can promote the proliferation of chondrocytes, stimulate the synthesis of matrix and prevent cartilage degeneration. However, its effect and the underlying mechanism for IVDD have not been fully revealed. Chondrogenic specific matrix components’ mRNA expression of nucleus pulposus cell (NPC) was determined by qPCR. Protein expression of NPC matrix components and protein kinase C was determined by Western blotting. A rat caudal intervertebral disc degeneration model was established and tested for calcitonin in vivo. IL‐1 induced NPC change via decreasing protein kinase C (PKC)‐ε phosphorylation, while increasing PKC‐δ phosphorylation. Calcitonin treatment could prevent or reverse IL‐1‐induced cellular change on PKC signalling associated with degeneration. The positive effect of calcitonin on IVDD in vivo was verified on a rat caudal model. In summary, this study, for the first time, elucidated the important role of calcitonin in the regulation of matrix components in the nucleus of the intervertebral disc. Calcitonin can delay degeneration of the intervertebral disc nucleus by activating the PKC‐ε pathway and inhibiting the PKC‐δ pathway.

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“…When the pressure is increased, the outflow of water increases and vice versa. Continuous increased pressure affects the water outflow and inflow of the intervertebral disc and causes disorders of material exchange of the intervertebral disc, thereby resulting in disc degeneration [23].…”

Section: Discussionmentioning

confidence: 99%

Biomechanical Effects of Fixation of Different Segments of Goat Lumbar Spine on Adjacent Segmental Motion and Intradiscal Pressure Change

Yin

et al. 2019

Med Sci Monit

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Background The aim of this study was to investigate the biomechanical fixation effects of different segments of the goat spine on adjacent segmental motion and intradiscal pressure (IDP) change. Material/Methods Eighteen goat spine specimens were randomly divided into 3 groups: group A (single-segment fixation), group B (double-segment fixation), and group C (triple-segment fixation). The motion was tested on each specimen using a spinal motion simulation test system with rational pressure loading. The IDP was measured using a pinhole pressure sensor. Results Range of motion (ROM) and IDP of adjacent segments increased with increased external load. In comparison of the 3 groups, significant differences in ROM were found when the external force was more than 100 N (P<0.05). The differences in IDP of the adjacent segment were statistically significant (P<0.05) when external pressure was greater than or equal to 60 N. However, in comparison of group A with group B, no significant differences in ROM and IDP of the adjacent segments were noted for the motions of anterior flexion, posterior extension, and lateral bending (P>0.05). Moreover, upper adjacent segments had greater ROM than the lower adjacent segments (P<0.05). We found significant differences between IDPs of the upper adjacent segments and lower adjacent segments (P<0.05). Conclusions As the number of fixated lumbar segments increases, ROM and IDP of the adjacent segments increase. Multisegment fixation is most likely the main factor contributing to the development of adjacent segmental lesions after lumbar fixation.

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Enhanced Type X Collagen Expression in the Extruded Nucleus Pulposus of the Chondrodystrophoid Dog

Cited by 9 publications

References 22 publications

Total disc replacement using tissue-engineered intervertebral discs in the canine cervical spine

Total disc replacement using tissue-engineered intervertebral discs in the canine cervical spine

Calcitonin inhibits intervertebral disc degeneration by regulating protein kinase C

Biomechanical Effects of Fixation of Different Segments of Goat Lumbar Spine on Adjacent Segmental Motion and Intradiscal Pressure Change

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