Mobile devices have gained more attention from the society that is using them increasingly for a variety of purposes. For complete insertion of the population in this constant digital evolution it is fundamental that mobile applications also offer access to different user profiles, regardless of their disabilities or limitations. Considering quality, productivity, and speed of application creation, there is a wide range of good development practices and evaluations. However, methods that involve usability and accessibility are still developing. The purpose of this article is to present the Acc-MobileCheck, which is a checklist of accessibility and usability for mobile devices apps, based on good software development practices and guided by Design Patterns. The Acc-MobileCheck, composed of 47 verification items, consists of questions that look for evidence of problems of comprehension, operation, perception, and adaptation in mobile apps. The evaluation method aims to address difficulties that can be faced by people with hearing, visual, intellectual, or mobility impairment. Five experts and three developers of mobile apps had evaluated the Acc-MobileCheck. The conclusive results show that the checklist is usable and includes essential issues for the evaluation of accessibility and usability. The data obtained allowed a restructuring of the evaluation method developed, and the positive comments about the checklist demonstrate its adequacy to attend the demand.