Enhanced Urinary Immunoreactive Thromboxane in neonatal Necrotixing Enterocolitis

Hyman, Paul E.

doi:10.1001/archpedi.1987.04460060102046

Cited by 20 publications

(7 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The site of platelet consumption in NEC remains to be identified, but is widely presumed to be related to microthrombotic events in the inflamed bowel (8, 37). Existing information from animal models indicate that circulating platelets are likely exposed to diverse activators including bacterial products, platelet-activating factor, arachidonic acid metabolites, various factors from the coagulation cascades (38, 39).…”

Section: Discussionmentioning

confidence: 99%

Neonatal mice with necrotizing enterocolitis-like injury develop thrombocytopenia despite increased megakaryopoiesis

et al. 2017

View full text Add to dashboard Cite

Background Thrombocytopenia is frequently encountered in infants with necrotizing enterocolitis (NEC). To develop a preclinical model of NEC-related thrombocytopenia, we measured serial platelet counts in 10-day-old (P10) mouse pups with trinitrobenzene sulfonic acid (TNBS)-induced NEC-like injury. We also measured platelet volume indices, immature platelet fraction (IPF), and megakaryocyte number/ploidy in these animals. Methods Platelet counts, platelet volume indices, and IPF were measured in control (N=65) and TNBS-treated pups (N=104) using an automated hematology analyzer. Bone marrow megakaryocyte number, ploidy and CD41 expression were measured by flow cytometry. These findings were confirmed in a small cohort of P3 mice with NEC-like injury. Results Murine pups with TNBS-mediated NEC-like injury developed thrombocytopenia at 15–24h after exposure to TNBS. Intestinal injury was associated with increased platelet volume indices (mean platelet volume, platelet-to-large cell ratio, and platelet distribution width), and IPF, indicating increased thrombopoiesis. These mice also showed increased megakaryocyte number, ploidy, and CD41 expression, indicating increased megakaryocyte differentiation. Conclusions Similar to human NEC, murine NEC-like injury was also associated with decreased platelet counts. There was evidence of increased megakaryocyte differentiation and thrombopoiesis, which favors peripheral consumption of platelets as the likely mechanism of thrombocytopenia in these animals, over decreased platelet production.

show abstract

Section: Discussionmentioning

confidence: 99%

Neonatal mice with necrotizing enterocolitis-like injury develop thrombocytopenia despite increased megakaryopoiesis

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Bowel inflammation leads to microthrombotic events and enhances the increased demand of platelets. 28 During thrombus formation, platelet factor 4 is released, which negatively regulates megakaryopoiesis in vivo. 29 So far, it is an active area of investigation whether platelet transfusions accelerate this process by the release of cytokines and thus increase bowel inflammation.…”

Section: Discussionmentioning

confidence: 99%

Low immature platelet fraction suggests decreased megakaryopoiesis in neonates with sepsis or necrotizing enterocolitis

et al. 2013

View full text Add to dashboard Cite

show abstract

“…For example, elevations of urinary thromboxane A2, and serum IL-1, IL-6, TNF, ET-1, and PAF have been associated with the presence of NEC in the acute stages of disease [Caplan et al, 1990b;Harris et al, 1994;Hyman et al, 1987]. In clinical studies, samples from NEC patients demonstrate elevated levels of various inflammatory mediators compared to gestational-age matched controls.…”

Section: Role Of Inflammation In Necmentioning

confidence: 99%

Necrotizing Enterocolitis: Insights into the Pathogenesis of this Challenging Disease

Caplan¹

2013

Colloquium Series on Integrated Systems Physiology: From Molecule to Function

View full text Add to dashboard Cite

Enhanced Urinary Immunoreactive Thromboxane in neonatal Necrotixing Enterocolitis

Cited by 20 publications

References 19 publications

Neonatal mice with necrotizing enterocolitis-like injury develop thrombocytopenia despite increased megakaryopoiesis

Neonatal mice with necrotizing enterocolitis-like injury develop thrombocytopenia despite increased megakaryopoiesis

Low immature platelet fraction suggests decreased megakaryopoiesis in neonates with sepsis or necrotizing enterocolitis

Necrotizing Enterocolitis: Insights into the Pathogenesis of this Challenging Disease

Contact Info

Product

Resources

About