Enhanced whole-cell biotransformation of 3-chloropropiophenone into 1-phenyl-1-propanone by hydrogel entrapped Chlorella emersonii (211.8b)

Khan, Sikandar; Fu, Pengcheng; Fonzo, Alessandro Di; Marasca, Irene; Secundo, Francesco

doi:10.1007/s10529-021-03194-y

Cited by 1 publication

(1 citation statement)

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“…In contrast to cyanobacteria, which have been used in various studies for biocatalysis, examples employing green algae are scarce ( Zheng et al, 2018 ), which is mostly due to the limited molecular biology toolbox for such strains. Chlorella vulgaris or Chlamydomonas reinhardtii were recently applied as whole-cell biocatalysts, using the photosynthetic reduction power for either dehalogenations ( Khan et al, 2021 ) or aliphatic amine formation via an amine dehydrogenase ( Löwe et al, 2018 ; Gröger, 2019 ). The presented examples indicate a limited applicability of phototrophs for NADH-dependent reactions, which only indirectly couple to the photosynthetic light reaction.…”

Section: Pathway Engineeringmentioning

confidence: 99%

Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis

Theodosiou

Tüllinghoff

Toepel

et al. 2022

Front. Bioeng. Biotechnol.

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The successful realization of a sustainable manufacturing bioprocess and the maximization of its production potential and capacity are the main concerns of a bioprocess engineer. A main step towards this endeavor is the development of an efficient biocatalyst. Isolated enzyme(s), microbial cells, or (immobilized) formulations thereof can serve as biocatalysts. Living cells feature, beside active enzymes, metabolic modules that can be exploited to support energy-dependent and multi-step enzyme-catalyzed reactions. Metabolism can sustainably supply necessary cofactors or cosubstrates at the expense of readily available and cheap resources, rendering external addition of costly cosubstrates unnecessary. However, for the development of an efficient whole-cell biocatalyst, in depth comprehension of metabolic modules and their interconnection with cell growth, maintenance, and product formation is indispensable. In order to maximize the flux through biosynthetic reactions and pathways to an industrially relevant product and respective key performance indices (i.e., titer, yield, and productivity), existing metabolic modules can be redesigned and/or novel artificial ones established. This review focuses on whole-cell bioconversions that are coupled to heterotrophic or phototrophic metabolism and discusses metabolic engineering efforts aiming at 1) increasing regeneration and supply of redox equivalents, such as NAD(P/H), 2) blocking competing fluxes, and 3) increasing the availability of metabolites serving as (co)substrates of desired biosynthetic routes.

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