Enhanced wound healing effect of canine adipose-derived mesenchymal stem cells with low-level laser therapy in athymic mice

Kim, Hyoju; Choi, Kyuseok; Kweon, Oh‐Kyeong; Kim, Wan Hee

doi:10.1016/j.jdermsci.2012.09.013

Cited by 66 publications

(67 citation statements)

References 38 publications

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“…While mesenchymal stem cells have been observed to have a stimulatory effect on hair regeneration due to the paracrine secretion of growth factors in previous studies [10, 22], we expected DPLTs to be the direct structural components of the hair follicle as a substitute for DPs under control of EMI. Injected fluorescence-labeled DPLTs, however, did not constitute regenerated DPs.…”

Section: Discussionmentioning

confidence: 92%

Hair growth promoting effect of dermal papilla like tissues from canine adipose-derived mesenchymal stem cells through vascular endothelial growth factor

Lee

Bae

Lee

et al. 2016

The Journal of Veterinary Medical Science

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The purpose of this study was to investigate the protein expression pattern and the in vivo trichogenicity of dermal papilla-like tissues (DPLTs) made from canine adipose-derived mesenchymal stem cells (ASCs) in athymic nude mice. Canine ASCs were isolated and cultured from adipose tissue, and differentiation was induced by culturing ASCs in dermal papilla forming media. DPLTs were embedded in collagen gel, and their structural characteristics and protein expression were evaluated by hematoxylin and eosin stain and immunohistochemistry. Athymic nude mice were divided into two groups (control and DPLTs groups), and DPLTs were injected in skin wounds of mice in the DPLTs group. The trichogenicity of DPLTs was assessed by gross and histological evaluations for 30 days. The fate and the growth factor-secretion effect of DPLTs were evaluated by immunohistochemistry and Western blotting. DPLTs have a compact aggregated structure, form extracellular matrix and highly express the protein specific for dermal papillae, including ALP and versican. New hair follicle formation was remarkable in nude mice of the DPLTs group in gross findings and H&E stain. Vascularization was increased in the DPLTs group, which was the effect of vascular endothelial growth factor secreted by DPLTs in vitro and in vivo. These data suggest that engineered canine DPLTs have characteristics of dermal papillae and have a positive effect on hair regeneration by secreting growth factors.

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Section: Discussionmentioning

confidence: 92%

Hair growth promoting effect of dermal papilla like tissues from canine adipose-derived mesenchymal stem cells through vascular endothelial growth factor

Lee

Bae

Lee

et al. 2016

The Journal of Veterinary Medical Science

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“…Hydrogels have been shown to maintain ASC viability within the wound, thereby prolonging their healing capacities for a longer period of time [125]. Decay in ASC healing properties may be mitigated by the use of low level laser therapy, which can act to stimulate these cells [126]. In terms of biomaterials, chitosan and PLCP-P123 (nonwoven nanofibrous material with structural similarity to ECM) scaffolds have been shown to be effective in the wound healing process mediated by ASCs [127][128][129].…”

Section: Wound Healingmentioning

confidence: 99%

The promising role for adipose-derived mesenchymal stem cells in tissue regeneration

Arun¹,

Lee²,

Brandacher³

et al. 2016

Nanomaterials and Regenerative Medicine

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“…Combination of low-level laser therapy (LLLT) with canine adipose-derived mesenchymal stem cells (ASCs) was attempted to obtain a sustainable effect in the recipient tissue [31]. LLLT increased the survival of ASCs with the decreased apoptosis of ASCs in the wound bed.…”

Section: Introduction Of Stem Cells Into Wound Tissuementioning

confidence: 99%

Attempts to accelerate wound healing

Kasuya

Tokura

2014

Journal of Dermatological Science

200

134

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Enhanced wound healing effect of canine adipose-derived mesenchymal stem cells with low-level laser therapy in athymic mice

Cited by 66 publications

References 38 publications

Hair growth promoting effect of dermal papilla like tissues from canine adipose-derived mesenchymal stem cells through vascular endothelial growth factor

Hair growth promoting effect of dermal papilla like tissues from canine adipose-derived mesenchymal stem cells through vascular endothelial growth factor

The promising role for adipose-derived mesenchymal stem cells in tissue regeneration

Attempts to accelerate wound healing

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