“…ex E. Vilm. (Malhotra and Maheshwari, 1977) and Nicotiana tabacum L. Reinert, 1980, 1981). Since then, cold-shock has been successfully employed in the obtention of haploid and double haploid plants in several species as diverse as Zea mays L., Triticum aestivum L., Hordeum vulgare L., and Oryza sativa L. among others (Aionesei et al, 2005, and references therein).…”