Enhancement by Interferon of the Expression of Surface Antigens on Murine Leukemia L 1210 Cells

Abstract

Preparations of mouse interferon enhanced the expression of surface antigens of murine leukemia L 1210 cells, as determined by their alloantibodyabsorbing capacity. The factor responsible for the enhancement of surface antigen expression could not be dissociated from the antiviral activity of interferon by standard physicochemical means. Likewise, interferon did not increase the antibody-absorbing capacity of an interferonresistant subline of L 1210 cells. We conclude that interferon treatment of L 1210 cells is accompanied by modifications of the cell surface.In addition to the well-known antiviral action (1), interferon preparations also exert various biologic effects on cells (2-17). The nature of some of these effects suggested the possibility that interferon treatment might be associated with modifications of the cell surface. To test this hypothesis we have determined the effect of interferon on the surface antigen expression of mouse leukemia L 1210 cells by measuring their alloantibody-absorbing capacity. We report herein the results of these experiments. MATERIALS AND METHODSCell Culture. Murine leukemia L 1210 cells (18) and an interferon-resistant subline, L 1210-R (19), were cultivated in nonagitated suspension cultures in nutrient medium RPMI 1640 (Gibco), supplemented with 20% heat-inactivated horse serum and 1% i-glutamine (10 mmol/ml). EL4 cells (provided by Dr. K. T. Brunner) were cultivated in suspension cultures in the same nutrient medium, supplemented with 10% heat-inactivated calf serum, glutamine, asparagine (36 mg/liter) folic acid (6 mg/liter), and 5% trypticase soy broth.Interferon and Control Preparations. Preparations of mouse interferon were obtained from the nutrient medium of monolayer cultures of MSV-Ia (20), or C-243 cells (21) (provided through the courtesy of Dr. S. Baron) inoculated with Newcastle disease virus (NDV), and from brains of C57BI/6 mice inoculated intracerebrally with West Nile virus (22). Preparations of rabbit interferon were obtained from the nutrient medium of monolayer cultures of RK 13 cells, inoculated with NDV. Control preparations consisted of the medium of uninoculated cell cultures or tissues of uninoculated animals. Preparation and semipurification of murine interferon have been described (15 (L 1210 and EL4 cells were maintained by serial passage in ascitic form in DBA/2 and C57B1/6 mice, respectively). The mice were bled 1 week after the last injection. The sera were decomplemented and stored at -20°. Isoantisera prepared by intraperitoneal injection of C57B1/6 mice with Balb/c splenic lymphocytes or intraperitoneal injection of Balb/c mice with C57B1/6 splenic lymphocytes were generously provided by Dr. J. P. Levy.Labeling of Target Cells. For labeling with radioactive chromium (56Cr), 1 ml of a cell suspension (5 X 106 viable cells per ml) was incubated for 30 min at 370 with 0.1 ml of Na2CrO4 (2 mCi/ml, specific activity 400 Ci/mg of chromium, Centre d'Energie Atomique, France) (24).Cytotoxic Assay. 1 ml of 5"Cr-labeled target cells (2 X 1...

The clinical and physiological spectrum of interferon‐alpha induced thyroiditis

“…85,91 In addition, IFN␣ has been shown in some studies to modify immunoglobulin production and to decrease T regulatory cell function. 92,93 A greater incidence of Th1 activity has been demonstrated in organ specific autoimmunity. 94,95 One hypothesis on the mechanism of IIT is that IFN␣ shifts the immune response to a Th1 mediated pattern, 96 resulting in the production of IFN-␥ and IL-2, two potent proinflammatory cytokines.…”

Electrophoretically pure mouse interferon exerts multiple biologic effects.

“…Enhanced expression of H-2 antigens by interferontreated cells (17) As shown in Fig. 3 Six-week-old female BALB/c mice were injected i.p.…”

“…After 7 days, the total number of EA cells in the peritoneal cavity of each mouse was determined by using techniques previously described (7). There were six mice per group.Enhanced expression of H-2 antigens by interferontreated cells (17) As shown in Fig. 3 Six-week-old female BALB/c mice were injected i.p.…”

“…In addition to interferon's well-known antiviral action, preparations of it have been reported to exert multiple effects on cells [i.e., inhibition of the multiplication of bormal and tumor cells in culture (2)(3)(4)(5), inhibition of the growth of a variety of transplantable tumors in mice (6)(7)(8), modulation of the synthesis and release of specific cellular products (9)(10)(11)(12)(13)(14)(15)(16), modification of the cell surface (17)(18)(19)(20)(21), and modulation of both the humoral (22)(23)(24)(25)(26)(27) and cell-mediated immune responses (28)(29)(30)(31)(32)(33)(34)]. Although experimental results support the hypothesis that the antiviral action of interferon is only one manifestation of its effect on cells (35)(36)(37), it is important to emphasize that these experiments were undertaken using preparations in which interferon protein constituted at most only 1% of the total protein content.…”

Administration of subtumor regression dosage of TNF-α to mice with pre-existing parental tumors augments the vaccination effect of TNF gene-modified tumor through the induction of MHC class I molecule

“…2,4,[11][12][13][14][15][16][17][18] This effect may partly explain the reduced tumorigenicity of cytokine gene transfectants, which are exposed to high levels of locally secreted cytokines through the autocrine manner.…”