Enhancement effect of essential oils from the fruits and leaves of Alpinia oxyphylla on skin permeation and deposition of indomethacin

Abstract

Essential oils from plants are gaining increasing attention as potential chemical penetration enhancers. This study aimed to investigate the enhancement effect of essential oils from the fruits and leaves of Alpinia oxyphylla on skin permeation and deposition of indomethacin. In vitro permeation experiments were performed in Franz-type cells through rat skin, and the amount of drug passing through into the receptor phase was analyzed by ultra performance liquid chromatography-photodiode array (UPLC-PDA). Ultra fast liquid chromatography coupled with tandem mass spectrometry (UFLC-MS/MS) was used to analyze the plasma drug concentration of indomethacin to examine the enhancement effect of essential oils in an in vivo rat model of drug delivery. Both oils demonstrated a significant enhancement effect on drug delivery and skin deposition (p < 0.05). Particularly, at 3% concentration, enhancement ratios of fruit oil and leaf oil were 10.16 and 4.61, respectively, which were both significantly higher than that of the commonly used enhancer, Azone (2.04). Major constituents of both oils were identified by gas chromatography-mass spectrometry (GC-MS). It may be deduced that higher content hydrocarbon terpenes in the fruit oil contribute to the increased enhancement effect relative to leaf oil. The skin irritation test indicated that both oils at certain concentrations (1%, 3%, and 5%) did not cause obvious erythema or edema in rabbits. Considering their enhanced drug permeation and low skin irritation, essential oils from Alpinia oxyphylla could be novel penetration enhancers and have promising applications in transdermal drug delivery and cosmetics.

Quality evaluation of Alpinia oxyphylla after Aspergillus flavus infection for storage conditions optimization

“…Of these, nootkatone (10.23–12.30%), p-cymene (5.88–8.77%), alloaromadendrene (8.72–10.34%), aristolene epoxide (3.26–4.56%), α-selinene (1.87–2.43%), α-guaiene (1.85–2.31%), α-panasinsen (1.78–2.20%), caryophyllene oxide (1.85–2.49%), gingerol (2.04–2.94%) were found to be the principal chemical constituents. Similar results have been reported in other studies (Feng et al 2015 ; Miao et al 2015 ). As shown in Additional file 1 : Figure S3 and Table 2 , mold infection had small effects on the composition of volatile compounds in A. oxyphylla .…”

“…In addition, A. oxyphylla is also consumed as a food in China. Phytochemical studies have indicated that it contains volatile oils, polysaccharides, flavonoids and diarylheptanoids (Li et al 2013 ; Feng et al 2015 ; Zhao et al 2013 ). Because it is widely distributed in tropical and subtropical regions, A. oxyphylla can become moldy during its growth, harvesting, processing and storage.…”

Natural Monoterpenes: Much More than Only a Scent

“…A still further example is the permeation effects of isopulegol, 1,8‐cineole and limonene, menthol and Alpinia oxyphylla Miq . oil …”

Qualitative and quantitative analysis of Alpiniae Oxyphyllae Fructus by high‐performance liquid chromatography coupled to Fourier transform‐ion cyclotron resonance mass spectrometry

“…Alpiniae Oxyphyllae Fructus ("Yi-Zhi-Ren" in Chinese), the mature fruit of Alpinia oxyphylla Miq., is a famous traditional Chinese medicine (TCM) [1]. Alpiniae have reported that Alpiniae Oxyphyllae Fructus has the pharmacological activities of protecting the kidneys, protecting the nervous system, regulating immune function, regulating gastrointestinal function, anti-oxidation, anti-inflammatory, and protecting the cardiovascular system [4][5][6][7][8][9][10][11][12].…”

“…Up to now, it has been extensively used as a dietary and medicinal substance in China for thousands of years [2,3]. Numerous studies have reported that Alpiniae Oxyphyllae Fructus has the pharmacological activities of protecting the kidneys, protecting the nervous system, regulating immune function, regulating gastrointestinal function, anti‐oxidation, anti‐inflammatory, and protecting the cardiovascular system [4–12].…”