Enhancement of a culture‐based bacterial detection system (eBDS) for platelet products based on measurement of oxygen consumption

Holme, Stein; McAlister, Morven; Ortolano, Girolamo A.; Chong, Chiyong; Cortus, Mary Anne; Jacobs, Michael; Yomtovían, Roslyn; Freundlich, Lawrence F.; Wenz, Barry

doi:10.1111/j.1537-2995.2005.04405.x

Cited by 41 publications

(46 citation statements)

References 13 publications

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“…All of the results with an O 2 concentration below a threshold of 9.4% are interpreted as positive. The analytical sensitivity has been investigated in several studies [22][23][24]64] and is comparable to the BacT/ALERT sensitivity with 1 CFU/ml. The advantage of this bacterial detection assay is that it is a barcode-controlled, closed system to avoid secondary contamination, and it has a fixed detection time of 24 h after sample collection.…”

Section: Discussionmentioning

confidence: 99%

“…Additional culture systems, such as the Bactec™ system (BD Diagnostics -Diagnostic Systems, Becton Dickinson GmbH, Heidelberg, Germany), were also available on the market and were implemented in some of the blood donor services with comparable data [19][20][21]. The Pall eBDS system [19,[22][23][24] (Pall GmbH, Dreieich, Germany) must be grouped between the culture methods and the rapid detection methods. In this system, a small sample volume is incubated in special growth medium for 24 h. Thereafter, the O 2 consumption is analysed.…”

Section: Culture Methods In Combination With a 'Negative-to-date' Conmentioning

confidence: 99%

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Implementation of Bacterial Detection Methods into Blood Donor Screening – Overview of Different Technologies

Schmidt

Sireis

Seifried³

2011

Transfus Med Hemother

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Background: Through the implementation of modern technology, such as nucleic acid testing, over the last two decades, blood safety has improved considerably in that the risk of viral infection is less than 1 in a million blood transfusions. By contrast, the residual risk of transfusion-associated bacterial infection is stable at approximately 1 in 2,000 to 1 in 3,000 in platelets. To improve blood safety with regard to bacterial infections, many countries have implemented bacterial screening methods as part of their blood donor screening programmes. Methods: Bacterial detection methods are clustered into three groups: i) culture methods in combination with the ‘negative-to-date’ concept, ii) rapid detection systems with a late sample collection, and iii) bedside screening tests. Results: The culture methods are convincing because of their very high analytical sensitivity. Nevertheless, false-negative culture results and subsequent fatalities were reported in several countries. Rapid bacterial systems are characterised as having short testing time but reduced sensitivity. Sample errors are prevented by late sample collection. Finally, bedside tests reduce the risk for sample errors to a minimum, but testing outside of blood donation services may have risks for general testing failures. Conclusion: Bacterial screening of blood products, especially platelets, can be performed using a broad range of technologies. Each system exhibits advantages and disadvantages and offers only a temporary solution until a general pathogen inactivation technology is available for all blood components.

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Section: Discussionmentioning

confidence: 99%

Section: Culture Methods In Combination With a 'Negative-to-date' Conmentioning

confidence: 99%

Implementation of Bacterial Detection Methods into Blood Donor Screening – Overview of Different Technologies

Schmidt

Sireis

Seifried³

2011

Transfus Med Hemother

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“…This would allow most bacteria to multiply and ensure that they are detected. 12,13 Such a procedure is, however, at the expense of the shelf life of the PCs, which in Europe is limited to a maximum of 7 days in conjunction with a method to detect or reduce bacterial contamination. 14 Alternatives to sterility testing are inactivation procedures that reduce the load of bacteria besides other pathogens.…”

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confidence: 99%

Sterilization of platelet concentrates at production scale by irradiation with short‐wave ultraviolet light

et al. 2009

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“…Reflecting these concerns, the American Association of Blood Banks implemented a directive stating that blood banks and transfusion services shall have methods to limit and detect bacterial contamination in all platelet components after March 2004 (10,11 ). The different methods for bacterial screening can be categorized into culture and rapiddetection methods (12)(13)(14)(15)(16)(17). Culture methods, which require a long time to demonstrate the presence of bacteria, have remained the preferred way for platelet bacteria screening, although bacteria in PCs have been detected with other approaches (18 ).…”

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confidence: 99%

High-Volume Extraction of Nucleic Acids by Magnetic Bead Technology for Ultrasensitive Detection of Bacteria in Blood Components

Störmer

Kleesiek

Dreier³

2007

Clinical Chemistry

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Background: Nucleic acid isolation, the most technically demanding and laborious procedure performed in molecular diagnostics, harbors the potential for improvements in automation. A recent development is the use of magnetic beads covered with nucleic acid-binding matrices. We adapted this technology with a broadrange 23S rRNA real-time reverse transcription (RT)-PCR assay for fast and sensitive detection of bacterial contamination of blood products. Methods: We investigated different protocols for an automated high-volume extraction method based on magnetic-separation technology for the extraction of bacterial nucleic acids from platelet concentrates (PCs). We added 2 model bacteria, Staphylococcus epidermidis and Escherichia coli, to a single pool of apheresisderived, single-donor platelets and assayed the PCs by real-time RT-PCR analysis with an improved primerprobe system and locked nucleic acid technology. Coamplification of human ␤ 2 -microglobulin mRNA served as an internal control (IC). We used probit analysis to calculate the minimum concentration of bacteria that would be detected with 95% confidence. Results: For automated magnetic bead-based extraction technology with the real-time RT-PCR, the 95% detection limit was 29 ؋ 10 3 colony-forming units (CFU)/L for S. epidermidis and 22 ؋ 10 3 CFU/L for E. coli. No false-positive results occurred, either due to nucleic acid

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Enhancement of a culture‐based bacterial detection system (eBDS) for platelet products based on measurement of oxygen consumption

Abstract: The eBDS demonstrated improved detection sensitivity in the range of 1 to 15 CFUs per mL with no observed false-positives compared to the original BDS (detection range 100 to 500 CFUs/mL) with no false-positives.

Cited by 41 publications

References 13 publications

Implementation of Bacterial Detection Methods into Blood Donor Screening – Overview of Different Technologies

Implementation of Bacterial Detection Methods into Blood Donor Screening – Overview of Different Technologies

Sterilization of platelet concentrates at production scale by irradiation with short‐wave ultraviolet light

High-Volume Extraction of Nucleic Acids by Magnetic Bead Technology for Ultrasensitive Detection of Bacteria in Blood Components

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