Enhancement of adipose tissue formation by implantation of adipogenic-differentiated preadipocytes

Cho, Seung Woo; Kim, Inok; Kim, Su Hyang; Rhie, Jong Won; Choi, Cha Yong; Kim, Byung Soo

doi:10.1016/j.bbrc.2006.04.089

Cited by 95 publications

(85 citation statements)

References 26 publications

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“…This is in contrast with several studies that have shown that adMSC can differentiate into adipocytes even when implanted alone, 8,49 although the extent of this conversion is not large. While others have focused on predifferentation of adMSC in vitro and growth factor delivery (i.e., basic fibroblast growth factor) to enhance adipose tissue formation, 49 we focused on a revascularization strategy. The lack of adMSC differentiation when implanted alone without HMEC may be simply the result of cell death due to lack of revascularization seen without HMEC cotransplantation.…”

Section: Adipose Tissue Developmentcontrasting

confidence: 52%

Cotransplantation of Adipose-Derived Mesenchymal Stromal Cells and Endothelial Cells in a Modular Construct Drives Vascularization in SCID/bg Mice

Butler

Sefton

2012

Tissue Engineering Part A

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A modular approach to adipose tissue engineering was explored by embedding adipose-derived mesenchymal stromal cells (adMSC) in sub-mm-sized collagen rods or ''modules'' and coating with human microvascular endothelial cells (HMEC). After subcutaneous injection into a SCID/Bg mouse, HMEC on modules containing embedded adMSC appeared to detach from the modules to form vessels as early as day 3, as confirmed by the human EC-specific UEA-1 lectin stain, and these vessels persisted for up to 90 days. Vessel numbers decreased over 14 days, but vessel size increased suggesting a maturing of the vasculature. Vessel perfusion with the host was confirmed at 21 days by microCT. HMEC on modules without embedded adMSC remained attached to the module surface at day 3 and UEA-1 staining disappeared over 14 days suggesting cell death. It appeared that cotransplantation with adMSC had an anti-apoptotic and proangiogenic effect on HMEC. The early revascularization strategy may be successful in supporting adMSC viability and differentiation, as a preliminary study suggests progressive fat accumulation in the HMEC + adMSC implants: *60% of the implant area stained positive for Oil Red O by day 90. adMSC-embedded modules without HMEC surface coating did not show similar levels of Oil Red O staining. All implant volumes decreased over the time course of the experiment, yet HMEC + adMSC module implants were larger than adMSC-only implants at day 90. Collagen gel is mechanically weak and contracts in vivo making it unsuitable as a biomaterial for adipose tissue engineering where volume maintenance is critical. When combined with an appropriate biomaterial, the modular approach to adipose tissue engineering may represent a successful strategy to engineer soft tissue substitutes of clinical relevance.

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Section: Adipose Tissue Developmentcontrasting

confidence: 52%

Cotransplantation of Adipose-Derived Mesenchymal Stromal Cells and Endothelial Cells in a Modular Construct Drives Vascularization in SCID/bg Mice

Butler

Sefton

2012

Tissue Engineering Part A

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“…Adipogenic differentiation did not significantly affect the hLCAT delivery and the cell survival in this model using fibrin glue as a scaffold with m-ccdPA ( Figure 2B). In this context, our results may suggest that the transplanted cells with fibrin glue were differentiated into adipocytes without adipogenic pretreatment (Cho et al, 2006;Torio-Padron et al, 2007a). The immunohistochemical observation did not clearly show that ccdPA would undergo adipogenic differentiation after transplantation, but the transplanted ccdPA were clearly identified as hLCAT-delivery cells in the transplanted sites of the recipient mice ( Figures 3A-3F).…”

Section: Discussionmentioning

confidence: 78%

“…Several reports have shown that mouse (Mizuno et al, 2008) and human (Cho et al, 2006) preadipocytes after adipogenic induction were superior in survival potential when implanted into nude mice. We therefore examined whether adipogenic differentiation affects hLCAT delivery and the survival of m-ccdPA/lcat after implantation with fibrin glue.…”

Section: Transplantation Of M-ccdpa/lcat In Nude Micementioning

confidence: 99%

“…In addition, the secretion of vascular endothelial growth factor (a bioactive molecule secreted from the vascular system) around the transplanted graft in recipients is also important for long-term cell survival (Yamaguchi et al, 2005). Particularly, recent studies have highlighted the importance of various cytokines for the regulation of cell function and the surrounding matrix conditions (Kimura et al, 2003;Cho et al, 2006;Torio-Padron et al, 2007b;Kuramochi et al, 2008;Ning et al, 2009). Together with the consideration of cytokine delivery for the transplanted cells, the development of scaffolds for transplantation contributes to the early construction of the surrounding matrix around the transplanted site.…”

Section: Introductionmentioning

confidence: 99%

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Fibrin glue increases the cell survival and the transduced gene product secretion of the ceiling culture-derived adipocytes transplanted in mice

et al. 2011

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The development of clinically applicable scaffolds is important for the application of cell transplantation in various human diseases. The aims of this study are to evaluate fibrin glue in a novel protein replacement therapy using proliferative adipocytes and to develop a mouse model system to monitor the delivery of the transgene product into the blood and the fate of the transduced cells after transplantation. Proliferative adipocytes from mouse adipose tissue were transduced by a retroviral vector harboring the human lecithin-cholesterol acyltransferase (lcat) gene, and were subcutaneously transplanted into mice combined with fibrin glue. The lcat gene transduction efficiency and the subsequent secretion of the product in mouse adipocytes were enhanced using a protamine concentration of 500 μ g/ml. Adipogenesis induction did not significantly affect the lcat gene-transduced cell survival after transplantation. Immunohistochemistry showed the ectopic enzyme production to persist for 28 days in the subcutaneously transplanted genetransduced adipocytes. The increased viability of transplanted cells with fibrin glue was accompanied with the decrease in apoptotic cell death. The immunodetectable serum LCAT levels in mice implanted with the fibrin glue were comparable with those observed in mice implanted with Matrigel, indicating that the transplanted lcat gene-transduced adipocytes survived and functioned in the transplanted spaces with fibrin glue as well as with Matrigel for 28 days. Thus, this in vivo system using fibrin is expected to serve as a good model to further improve the transplanted cell/scaffold conditions for the stable and durable cell-based replacement of defective proteins in patients with LCAT deficiency.

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“…After 3 days of induction period, the cells were fed the same medium without isobutylmethylxanthine or indomethacin. 22 …”

Section: Cell Isolationmentioning

confidence: 99%

In vitro cardiomyogenic differentiation of adipose‐derived stromal cells using transforming growth factor‐β1

Gwak

Bhang

Yang

et al. 2009

Cell Biochemistry & Function

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Transplanting stem cells differentiated towards a cardiac lineage can regenerate cardiac muscle tissues to treat myocardial infarction. In this study, we tested the hypothesis that transforming growth factor-b1 (TGF-b1) induces cardiomyogenic differentiation of adiposederived stromal cells (ADSCs) in vitro. Rat ADSCs were cultured with TGF-b1 (10 ng ml À1 ) for 2 weeks in vitro. ADSCs cultured without TGF-b1 served as a control. The mRNA expression of cardiac-specific gene was induced by TGF-b1, while the control culture did not show cardiac-specific gene expression. Immunocytochemical analyses showed that a small fraction of ADSCs cultured with TGF-b1 for 2 weeks stained positively for cardiac myosin heavy chain (MHC) and a-sarcomeric actin. Flow cytometric analyses showed that the proportion of cells expressing cardiac MHC increased with TGF-b1. However, no mesenchymal differentiation (e.g., osteogenic and adipogenic differentiation) was detected other than cardiomyogenic differentiation. These results showed that TGF-b1 induce ADSC cardiomyogenic differentiation in vitro, which could be useful for myocardial infarction stem cell therapy.

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Enhancement of adipose tissue formation by implantation of adipogenic-differentiated preadipocytes

Cited by 95 publications

References 26 publications

Cotransplantation of Adipose-Derived Mesenchymal Stromal Cells and Endothelial Cells in a Modular Construct Drives Vascularization in SCID/bg Mice

Cotransplantation of Adipose-Derived Mesenchymal Stromal Cells and Endothelial Cells in a Modular Construct Drives Vascularization in SCID/bg Mice

Fibrin glue increases the cell survival and the transduced gene product secretion of the ceiling culture-derived adipocytes transplanted in mice

In vitro cardiomyogenic differentiation of adipose‐derived stromal cells using transforming growth factor‐β1

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