Enhancement of amygdalin activated with β-d-glucosidase on HepG2 cells proliferation and apoptosis

Zhou, Cunshan; Qian, Lichun; Ma, Haile; Yu, Xiaojie; Zhang, Youzuo; Qu, Wenjuan; Zhang, Xiaoxu; Xia, Wei

doi:10.1016/j.carbpol.2012.05.073

Cited by 79 publications

(58 citation statements)

References 29 publications

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“…8 Table 4. Similar to other recent studies (Nie et al, 2013;Zhou et al, 2012), β-glucosidase-mediated hydrolysis of amygdalin significantly enhanced its inhibitory effects on growth. The D-amygdalin ratio did not alter cytotoxicity in the absence of β-glucosidase.…”

Section: Cytotoxicity Assaysupporting

confidence: 90%

Stereoselective metabolism of amygdalin-based study of detoxification of Semen Armeniacae Amarum in the Herba Ephedrae–Semen Armeniacae Amarum herb pair

Song

Tang

et al. 2016

Journal of Ethnopharmacology

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Section: Cytotoxicity Assaysupporting

confidence: 90%

Stereoselective metabolism of amygdalin-based study of detoxification of Semen Armeniacae Amarum in the Herba Ephedrae–Semen Armeniacae Amarum herb pair

Song

Tang

et al. 2016

Journal of Ethnopharmacology

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“…β-Glucosidase also could synergistically act with the endo-β-1,4-glucanase (EC 3.2.1.4) and cellobiohydrolase (EC 3.2.1.91) to completely hydrolyze the cellulose. β-Glucosidases have been widely applied in various industrial fields such as food, feed, textile, detergents, pharmaceutical, and bio-ethanol production [3][4][5][6]. In addition, some β-glucosidases with transglycosylation activity can be used to produce the high-value-added rare oligosaccharides [7,8].…”

mentioning

confidence: 99%

Heterologous Expression and Characterization of a GH3 β-Glucosidase from Thermophilic Fungi Myceliophthora thermophila in Pichia pastoris

Zhao

Guo

Tian

et al. 2015

Appl Biochem Biotechnol

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A novel β-glucosidase of glycoside hydrolase (GH) family 3 from Myceliophthora thermophila (mtbgl3b) was successfully expressed in Pichia pastoris. The full-length gene consists of 2613 bp nucleotides encoding a protein of 870 amino acids. MtBgl3b showed maximum activity at pH 5.0 and remained more than 70 % relative activity at 3.5-6.0. The enzyme displayed the highest activity at 60 °C and kept about 90 % relative activity for 50-65 °C; besides, the enzyme showed psychrophilic trait and remains 51 % relative activity at 40 °C. MtBgl3b exhibited good stability over a wide pH range of 3.0-10.0 and was thermostable at 60 and 65 °C. The enzyme displayed highest activity towards p-nitrophenyl-β-D-glucopyranoside (pNPG), followed by p-nitrophenyl-D-cellobioside (pNPC), cellotetraose, cellotriose, cellobiose, and gentiobiose. When using 10 % cellobiose (w/v) as the substrate, the enzyme showed transglycosylation activity to produce the cellotriose. The kinetic parametric of K m and V max were 2.78 mM and 927.9 μM mg(-1) min(-1), respectively. Finally, the reaction mode of the enzyme and the substrates were analyzed by molecular docking approach.

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“…amygdalin may significantly reduce plasma viscosity, prolong thromboplastin time and reduce fibrinogen levels, whilst exerting few effects on whole blood viscosity (31). Amygdalin has also been used for the treatment of asthma, tumors, bronchitis, emphysema, leprosy and diabetes (32). Clinical observations have indicated the efficacy of THSWD in internal medicine, gynecology, andriatrics, dermatology and ophthalmology (33).…”

Section: Discussionmentioning

confidence: 99%

Network pharmacology-based prediction of the multi-target capabilities of the compounds in Taohong Siwu decoction, and their application in osteoarthritis

Zheng

et al. 2013

Experimental and Therapeutic Medicine

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Taohong Siwu decoction (THSWD), a formulation prescribed in traditional Chinese medicine (TCM), has been widely used in the treatment of osteoarthritis (OA). TCM has the potential to prevent diseases, such as OA, in an integrative and holistic manner. However, the system-level characterization of the drug-target interactions of THSWD has not been elucidated. In the present study, we constructed a novel modeling system, by integrating chemical space, virtual screening and network pharmacology, to investigate the molecular mechanism of action of THSWD. The chemical distribution of the ligand database and the potential compound prediction demonstrated that THSWD, as a natural combinatorial chemical library, comprises abundant drug-like and lead-like compounds that may act as potential inhibitors for a number of important target proteins associated with OA. Moreover, the results of the ‘compound-target network’ analysis demonstrated that 19 compounds within THSWD were correlated with more than one target, whilst the maximum degree of correlation for the compounds was seven. Furthermore, the ‘target-disease network’ indicated that THSWD may potentially be effective against 69 diseases. These results may aid in the understanding of the use of THSWD as a multi-target therapy in OA. Moreover, they may be useful in establishing other pharmacological effects that may be brought about by THSWD. The in silico method used in this study has the potential to advance the understanding of the molecular mechanisms of TCM.

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Enhancement of amygdalin activated with β-d-glucosidase on HepG2 cells proliferation and apoptosis

Cited by 79 publications

References 29 publications

Stereoselective metabolism of amygdalin-based study of detoxification of Semen Armeniacae Amarum in the Herba Ephedrae–Semen Armeniacae Amarum herb pair

Stereoselective metabolism of amygdalin-based study of detoxification of Semen Armeniacae Amarum in the Herba Ephedrae–Semen Armeniacae Amarum herb pair

Heterologous Expression and Characterization of a GH3 β-Glucosidase from Thermophilic Fungi Myceliophthora thermophila in Pichia pastoris

Network pharmacology-based prediction of the multi-target capabilities of the compounds in Taohong Siwu decoction, and their application in osteoarthritis

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